{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T03:40:13Z","timestamp":1758858013211,"version":"3.44.0"},"reference-count":20,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T00:00:00Z","timestamp":1758240000000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Gene Medicine"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Prostate cancer remains a leading cause of cancer\u2010related mortality, with castration\u2010resistant and metastatic forms posing significant therapeutic challenges. Marine\u2010derived polysaccharides from <jats:styled-content style=\"fixed-case\"><jats:italic>Enteromorpha prolifera<\/jats:italic><\/jats:styled-content> (EP) have emerged as promising anticancer agents due to their unique bioactive properties. In this study, we investigated the antitumor effects of EP on prostate cancer cells and elucidated its underlying molecular mechanisms. EP demonstrated selective cytotoxicity against aggressive DU145 prostate cancer cells. Mechanistically, EP induced G1\/S cell cycle arrest, suppressed DNA synthesis, and inhibited epithelial\u2010mesenchymal transition. Cellular thermal shift assays confirmed EP\u2013Akt1 interaction, increasing the thermal stability of Akt1. These findings establish EP as a novel Akt1\u2010targeting agent that simultaneously blocks proliferative and metastatic pathways in prostate cancer. In conclusion, our results highlight the therapeutic potential of EP as a multitargeted, marine\u2010derived compound for prostate cancer treatment.<\/jats:p>","DOI":"10.1002\/jgm.70038","type":"journal-article","created":{"date-parts":[[2025,9,20]],"date-time":"2025-09-20T05:39:47Z","timestamp":1758346787000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Marine\u2010Derived <scp><i>Enteromorpha prolifera<\/i><\/scp> Polysaccharides Suppress Castration\u2010Resistant Prostate Cancer via Akt1 Inhibition"],"prefix":"10.1002","volume":"27","author":[{"given":"Pingfa","family":"Lin","sequence":"first","affiliation":[{"name":"School of Pharmacy Fujian Health College  Fuzhou Fujian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Na","family":"Yao","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology Fujian Medical University Union Hospital  Fuzhou Fujian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tingting","family":"Lin","sequence":"additional","affiliation":[{"name":"School of Pharmacy Fujian Medical University  Fuzhou Fujian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanhui","family":"Zou","sequence":"additional","affiliation":[{"name":"School of Pharmacy Fujian Health College  Fuzhou Fujian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weiling","family":"Lin","sequence":"additional","affiliation":[{"name":"School of Pharmacy Fujian Health College  Fuzhou Fujian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-1922-8213","authenticated-orcid":false,"given":"Yong","family":"Lin","sequence":"additional","affiliation":[{"name":"School of Pharmacy Fujian Health College  Fuzhou Fujian China"},{"name":"Science and Technology Service Center Fujian Health College  Fuzhou Fujian China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,9,19]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2023.04.021"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2024.122488"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-021-00828-5"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-022-01168-8"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2023.216288"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.28414"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1201750"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.14.9133"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-021-03504-2"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12964-023-01225-x"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.21037\/atm-21-4544"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13045-021-01137-8"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40643-024-00800-5"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jff.2024.106244"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnut.2021.747928"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.31083\/j.fbl2908300"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2022.09.055"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.apcsb.2022.11.008"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaa1002"},{"key":"e_1_2_10_21_1","first-page":"1","article-title":"The Role of CDC25C in Cell Cycle Regulation and Clinical Cancer Therapy: A Systematic Review","volume":"20","author":"Liu K.","year":"2020","journal-title":"Cancer Cell International"}],"container-title":["The Journal of Gene Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jgm.70038","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T03:26:46Z","timestamp":1758857206000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jgm.70038"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9]]},"references-count":20,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2025,9]]}},"alternative-id":["10.1002\/jgm.70038"],"URL":"https:\/\/doi.org\/10.1002\/jgm.70038","archive":["Portico"],"relation":{},"ISSN":["1099-498X","1521-2254"],"issn-type":[{"type":"print","value":"1099-498X"},{"type":"electronic","value":"1521-2254"}],"subject":[],"published":{"date-parts":[[2025,9]]},"assertion":[{"value":"2025-05-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-23","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-19","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70038"}}