{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T04:10:10Z","timestamp":1758859810478,"version":"3.44.0"},"reference-count":36,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,9,18]],"date-time":"2025-09-18T00:00:00Z","timestamp":1758153600000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Gene Medicine"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Nonalcoholic fatty liver disease (NAFLD) forms a global health burden with prominent incidence rates exceeding 26%, possessing a versatile spectrum of presentations starting from benign liver steatosis and deteriorating to hepatic fibrosis and even cirrhosis. In our study, we aimed to be the first to investigate the serum concentrations of miR\u2010574\u20105p and miR\u2010522 in individuals with NAFLD and link them to obesity and dyslipidemia. Eighty participants were enrolled in our study and split into two groups: The first group included 40 individuals diagnosed with NAFLD via clinical, imaging, and laboratory procedures (24 males and 16 females). The second group involved 40 healthy controls (22 males and 18 females). Venous blood samples were collected from all subjects. The miR\u2010574\u20105p and miR\u2010522 serum expressions were evaluated using quantitative real\u2010time PCR (qRT\u2010PCR). Our study data revealed a marked reduction in the mean miR\u2010574 expression levels simultaneous with an increase in the mean level of miR\u2010522 expression in NAFLD cases compared to controls (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001), as for the specificity and sensitivity tests for the miR\u2010522 and miR\u2010574\u20105p markers, they revealed sensitivities of 80% and 100%, respectively, along with specificities of 100% and 95%, respectively, at cutoff values of 1.136 and 0.891, respectively. The <jats:italic>p<\/jats:italic> value for both markers is less than 0.001. Serum miR\u2010574\u20105p and miR\u2010522 could serve as promising biomarkers in diagnosing NAFLD.<\/jats:p>","DOI":"10.1002\/jgm.70042","type":"journal-article","created":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T02:11:18Z","timestamp":1758247878000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["miRNA\u2010574\u20105p and miRNA\u2010522 as Potential Biomarkers in Nonalcoholic Fatty Liver Disease"],"prefix":"10.1002","volume":"27","author":[{"given":"Reham","family":"Fares","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Faculty of Medicine Fayoum University  Fayoum Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Asmaa","family":"Mohammed","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Faculty of Medicine Fayoum University  Fayoum Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahmoud\u00a0A.\u00a0F.","family":"Khalil","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Faculty of Pharmacy Fayoum University  Fayoum Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tarek\u00a0I.","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Faculty of Medicine Fayoum University  Fayoum Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shaimaa\u00a0A.","family":"Fathy","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine and Endocrinology, Faculty of Medicine Cairo University  Cairo Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amira","family":"Samy","sequence":"additional","affiliation":[{"name":"Department of Clinical and Chemical Pathology, Faculty of Medicine Cairo University  Cairo Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0136-7096","authenticated-orcid":false,"given":"Ahmed\u00a0S.\u00a0Doghish","family":"0000\u20100002\u20100136\u20107096","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Pharmacy Badr University in Cairo  Cairo Egypt"},{"name":"Department of Biochemistry and Molecular Biology, Faculty of Pharmacy (Boys) Al\u2010Azhar University  Cairo Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Randa","family":"Erfan","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Faculty of Medicine Cairo University  Cairo Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,9,18]]},"reference":[{"key":"e_1_2_14_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/iub.597"},{"key":"e_1_2_14_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jlr.2023.100485"},{"key":"e_1_2_14_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2020.527059"},{"key":"e_1_2_14_5_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmjgast-2021-000780"},{"key":"e_1_2_14_6_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.59588"},{"key":"e_1_2_14_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2023.126739"},{"key":"e_1_2_14_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10620-015-4002-4"},{"key":"e_1_2_14_9_1","article-title":"A Spotlight on the Interplay of Signaling Pathways and the Role of miRNAs in Osteosarcoma Pathogenesis and Therapeutic Resistance","volume":"245","author":"Doghish A. 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