{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T09:21:39Z","timestamp":1776158499677,"version":"3.50.1"},"reference-count":51,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,11,16]],"date-time":"2025-11-16T00:00:00Z","timestamp":1763251200000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82370656"],"award-info":[{"award-number":["82370656"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007219","name":"Natural Science Foundation of Shanghai Municipality","doi-asserted-by":"publisher","award":["23ZR1450900"],"award-info":[{"award-number":["23ZR1450900"]}],"id":[{"id":"10.13039\/100007219","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Gene Medicine"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Acute pancreatitis (AP) is a severe inflammatory disease. Transient receptor potential (TRP) channels have been reported to participate in various pathophysiological processes. However, the role of TRP channels in the AP remains unclear. Here, we investigated the involvement of TRP channels in AP.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We identified differentially expressed genes (DEGs) using the public AP datasets GSE3644 and GSE109227 and identified 20 hub genes. We analyzed their correlation with the TRP genes. Four DEGs related to TRP channels were selected, and functional enrichment analyses were conducted. An interaction network involving the transcription factors, microRNAs (miRNAs), and mRNA of the four TRP\u2010associated genes was established. To evaluate the diagnostic effectiveness, receiver operating characteristic (ROC) analysis and machine learning (ML) methods, such as support vector classifiers and random forest, were applied. Furthermore, we analyzed immune cell infiltration and its relationship with the four TRP genes. To confirm these bioinformatics findings, we performed in\u00a0vitro and in\u00a0vivo experiments using primary acinar cells and an experimental mouse model of AP.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Four TRP\u2010related genes, namely,\n                      <jats:italic>MCOLN1<\/jats:italic>\n                      ,\n                      <jats:italic>MCOLN3<\/jats:italic>\n                      ,\n                      <jats:italic>TRPM7<\/jats:italic>\n                      , and\n                      <jats:italic>TRPV4<\/jats:italic>\n                      , were selected for the correlation analysis. Functional analyses showed that these four genes are involved in cellular senescence and the calcium signaling pathway. A TF\u2013miRNA\u2013mRNA interaction network was constructed for the four TRP\u2010associated genes. The areas under the ROC curves (AUC) of\n                      <jats:italic>MCOLN1<\/jats:italic>\n                      ,\n                      <jats:italic>MCOLN3<\/jats:italic>\n                      ,\n                      <jats:italic>TRPM7<\/jats:italic>\n                      , and\n                      <jats:italic>TRPV4<\/jats:italic>\n                      were 0.833, 0.700, 1.0, and 1.0, respectively. ML algorithms performed well in predicting AP with AUCs \u2265\u20090.67.\n                      <jats:italic>TRPM7<\/jats:italic>\n                      ,\n                      <jats:italic>TRPV4<\/jats:italic>\n                      , and\n                      <jats:italic>MCOLN3<\/jats:italic>\n                      are associated with immune cell infiltration of AP tissues. Using experimental AP models, we found that the expression of three TRP\u2010related genes,\n                      <jats:italic>MCOLN1<\/jats:italic>\n                      ,\n                      <jats:italic>TRPM7<\/jats:italic>\n                      , and\n                      <jats:italic>TRPV4<\/jats:italic>\n                      , was significantly upregulated in mouse AP tissues. Specifically, TRPM7 knockdown or inhibition by its inhibitor 2\u2010APB significantly reduced pancreatic acinar cell damage, inflammatory mediator expression, and mRNA levels of senescence\u2010associated genes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our study suggests that TRP\u2010related genes play crucial roles in AP and may be promising biomarkers for predicting the pathogenesis of AP.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jgm.70057","type":"journal-article","created":{"date-parts":[[2025,11,17]],"date-time":"2025-11-17T06:48:51Z","timestamp":1763362131000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Identification of the Role of Transient Receptor Potential Channels in Acute Pancreatitis"],"prefix":"10.1002","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-2113-9313","authenticated-orcid":false,"given":"Shuangjun","family":"Shen","sequence":"first","affiliation":[{"name":"Department of Gastroenterology, Shanghai General Hospital Shanghai Jiao Tong University School of Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaowan","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Shanghai General Hospital Shanghai Jiao Tong University School of Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhiyuan","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Shanghai General Hospital Shanghai Jiao Tong University School of Medicine  Shanghai 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