{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,23]],"date-time":"2025-12-23T12:39:19Z","timestamp":1766493559244,"version":"3.48.0"},"reference-count":19,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,12,3]],"date-time":"2025-12-03T00:00:00Z","timestamp":1764720000000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82170653"],"award-info":[{"award-number":["82170653"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82470676"],"award-info":[{"award-number":["82470676"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Gene Medicine"],"published-print":{"date-parts":[[2025,12]]},"abstract":"ABSTRACT<\/jats:title>\n \n Background<\/jats:title>\n Ferroptosis and immune responses are catching more and more attention in inflammatory diseases. However, the specific function and mechanism of the popular ferroptosis\u2010related gene SAT1 in acute pancreatitis (AP) still remain unknown.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n Differentially expressed genes (DEGs) and the Weighted Gene Co\u2010Expression Network Analysis (WGCNA) were integrated to screen the core gene set related to AP. The characteristic gene was identified through three machine learning algorithms (LASSO, SVM\u2010REF, and RF). Experimental approaches were performed to validate the findings by using qRT\u2010PCR and immunohistochemistry (IHC).<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Machine learning combined with bioinformatics determined that the ferroptosis gene SAT1 was the key regulatory gene of AP. The expression level of SAT1 was positively correlated with the severity of AP (\n p<\/jats:italic>\n \u2009<\u20090.05), which was also involved in immune functions. Experiments in\u00a0vivo and in\u00a0vitro validated that SAT1 was significantly upregulated in murine AP models (\n p<\/jats:italic>\n \u2009<\u20090.01). Knockdown of SAT1 could alleviate inflammation responses by reducing IL1\n \u03b2<\/jats:italic>\n and IL6, especially TNF\n \u03b1<\/jats:italic>\n .\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n SAT1 is elevated in AP, serving as a potential diagnostic and prognostic biomarker. Down regulation of SAT1 can decrease the release of inflammatory factors. We identified SAT1 as a potential therapeutic target for AP.<\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/jgm.70063","type":"journal-article","created":{"date-parts":[[2025,12,4]],"date-time":"2025-12-04T07:49:42Z","timestamp":1764834582000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Ferroptosis\u2010Related Gene SAT1 Contributes to Immune Responses in Acute Pancreatitis"],"prefix":"10.1002","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1681-8830","authenticated-orcid":false,"given":"Kena","family":"Zhou","sequence":"first","affiliation":[{"name":"Department of Gastroenterology Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine Shanghai China"},{"name":"Shanghai Key Laboratory of Pancreatic Disease, Institute of Pancreatic Disease Shanghai Jiao Tong University School of Medicine Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Congbo","family":"Cai","sequence":"additional","affiliation":[{"name":"Ningbo Yinzhou No. 2 Hospital Zhejiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rong","family":"Wan","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine Shanghai China"},{"name":"Shanghai Key Laboratory of Pancreatic Disease, Institute of Pancreatic Disease Shanghai Jiao Tong University School of Medicine Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xingpeng","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine Shanghai China"},{"name":"Shanghai Key Laboratory of Pancreatic Disease, Institute of Pancreatic Disease Shanghai Jiao Tong University School of Medicine 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