{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T22:15:39Z","timestamp":1769638539252,"version":"3.49.0"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T00:00:00Z","timestamp":1767657600000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Gene Medicine"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Ovarian cancer remains a leading cause of gynecologic cancer mortality, in part due to the persistence of ovarian cancer stem cells (OCSCs) that drive tumor recurrence, metastasis, and drug resistance. Anisomycin, a natural antibiotic derived from\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Streptomyces coelicolor<\/jats:italic>\n                    <\/jats:styled-content>\n                    , has previously been shown to exert antitumor effects, but the mechanisms by which it targets OCSCs remain unclear. In this study, primary human OCSCs were isolated and treated with anisomycin to investigate its biological and molecular effects. Cell proliferation, apoptosis, migration, and colony formation were assessed in\u00a0vitro, and tumorigenicity was evaluated in xenograft mouse models. Transcriptomic, biochemical, and molecular assays were performed to identify downstream pathways. Anisomycin treatment markedly inhibited proliferation and promoted senescence and cell death of OCSCs. Mechanistically, anisomycin induced upregulation of microRNA\u2010340, which in turn suppressed the deSUMOylating enzyme SENP6. This repression increased SUMOylation of key senescence\u2010related proteins, including p53 and p16, leading to stabilization of their expression and enforcement of cell\u2010cycle arrest. Overexpression of microRNA\u2010340 reproduced these effects, both in\u00a0vitro and in\u00a0vivo, confirming its central role in mediating anisomycin activity. Bioinformatic analyses further revealed that expression of SENP6 and senescence\u2010associated genes correlated with disease progression and patient survival in ovarian cancer cohorts. These findings identify a previously unrecognized epigenetic mechanism by which anisomycin induces senescence and death in OCSCs, suggesting that targeting the microRNA\u2010340\/SENP6\/SUMOylation pathway may represent a promising therapeutic approach.\n                  <\/jats:p>","DOI":"10.1002\/jgm.70081","type":"journal-article","created":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T00:24:42Z","timestamp":1767745482000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Anisomycin Induces Senescence and Death of Ovarian Cancer Stem Cells Through the MicroRNA\u2010340\/SENP6\/SUMOylation Pathway"],"prefix":"10.1002","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0646-5971","authenticated-orcid":false,"given":"Lian","family":"Yang","sequence":"first","affiliation":[{"name":"Shanghai Geriatric Institute of Chinese Medicine Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ye","family":"Xia","sequence":"additional","affiliation":[{"name":"Center of Reproductive Medicine Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zeyu","family":"Cui","sequence":"additional","affiliation":[{"name":"Shanghai Geriatric Institute of Chinese Medicine Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanchen","family":"Lai","sequence":"additional","affiliation":[{"name":"Shanghai Geriatric Institute of Chinese Medicine Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongyi","family":"Huang","sequence":"additional","affiliation":[{"name":"School of Environmental and Chemical Engineering Shanghai University  Shanghai 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