{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T06:31:44Z","timestamp":1777357904456,"version":"3.51.4"},"reference-count":32,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,4,19]],"date-time":"2026-04-19T00:00:00Z","timestamp":1776556800000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Gene Medicine"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Pneumonia caused by\n                      <jats:italic>Klebsiella pneumonia<\/jats:italic>\n                      (\n                      <jats:italic>Kp<\/jats:italic>\n                      ) poses a significant risk to global public health. Vitamin D may reduce\n                      <jats:italic>Kp<\/jats:italic>\n                      infection risk and improve prognosis through immunomodulation.\n                    <\/jats:p>\n                    <jats:p>\n                      This study aimed to validate the treatment effects of Vitamin D and explore its regulatory mechanism in\n                      <jats:italic>Kp<\/jats:italic>\n                      \u2010pneumonia.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      In this study, a murine model of\n                      <jats:italic>Kp<\/jats:italic>\n                      \u2010induced pneumonia and the MH\u2010S alveolar macrophage cell line were used. Experimental assays included RT\u2010qPCR, Western blot, TUNEL assay, ELISA, flow cytometry, dual\u2010luciferase reporter assay, and metabolic analyses (FAO activity, Seahorse XF Glycolysis Stress Test).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The results showed that vitamin D administration mitigated\n                      <jats:italic>Kp<\/jats:italic>\n                      \u2010induced lung injury in mice. Mechanically, vitamin D alleviated inflammation by inhibiting macrophage M1 polarization. Vitamin D exerted its effects by upregulating miR\u2010223, which directly targeted and suppressed ACSL3 expression. In macrophages, miR\u2010223 overexpression alleviated macrophage apoptosis and M1 polarization by downregulating ACSL3. Knockdown of ACSL3 induced a shift to M2 polarization by enhancing FAO and suppressing glycolysis. In\u00a0vivo, miR\u2010223 overexpression alleviated\n                      <jats:italic>Kp<\/jats:italic>\n                      \u2010induced lung injury by downregulating ACSL3.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      In conclusion, vitamin D induces macrophage M2 polarization by upregulating miR\u2010223, which inhibits ACSL3, leading to lipid metabolism reprogramming. This novel axis represents a potential therapeutic strategy for\n                      <jats:italic>Kp<\/jats:italic>\n                      \u2010induced pneumonia.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jgm.70092","type":"journal-article","created":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T01:59:16Z","timestamp":1776650356000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Vitamin D Mitigates\n                    <scp>\n                      <i>Klebsiella pneumoniae<\/i>\n                    <\/scp>\n                    \u2010Induced Pneumonia by Regulating Macrophage Polarization Through miR\u2010223\/ACSL3 Axis\u2010Mediated Lipid Metabolism Reprogramming"],"prefix":"10.1002","volume":"28","author":[{"given":"Jiajia","family":"Hu","sequence":"first","affiliation":[{"name":"Department of MICU The Third Affiliated Hospital of Sun Yat\u2010sen University  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