{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T12:24:42Z","timestamp":1787574282414,"version":"build-2736575974"},"reference-count":33,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T00:00:00Z","timestamp":1786406400000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004775","name":"Natural Science Foundation of Gansu Province","doi-asserted-by":"publisher","award":["22JR11RA200"],"award-info":[{"award-number":["22JR11RA200"]}],"id":[{"id":"10.13039\/501100004775","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Gene Medicine"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>This study aims to elucidate the mechanism through which prostate cancer\u2010associated transcript 6 (PCAT6) modulates immune escape in triple\u2010negative breast cancer (TNBC), focusing on its interaction with IGF2BP1 and PD\u2010L1.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We analyzed 68 paired TNBC clinical specimens and utilized TNBC cells (BT\u2010549, MDA\u2010MB\u2010468) with lentivirus\u2010mediated gene manipulation. Functional assays included cell counting kit\u20108, EdU incorporation, Transwell migration\/invasion, and co\u2010culture with activated CD8\n                      <jats:sup>+<\/jats:sup>\n                      T cells. Immune function was assessed through LDH release, ELISA (IFN\u2010\u03b3 and granzyme B), and flow cytometry. Molecular interactions were investigated via RNA immunoprecipitation, RNA pulldown, fluorescence in\u00a0situ hybridization, and actinomycin D\u2010based mRNA stability assays.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      PCAT6 was markedly upregulated in TNBC tissues and cell lines, correlating with advanced tumor stage and lymph node metastasis. PCAT6 knockdown restrained tumor cell proliferation, migration, and invasion while downregulating PD\u2010L1 expression. In co\u2010culture systems, PCAT6 depletion enhanced CD8\n                      <jats:sup>+<\/jats:sup>\n                      T cell cytotoxicity, evidenced by increased inflammatory factor secretion and elevated IFN\u2010\u03b3\n                      <jats:sup>+<\/jats:sup>\n                      CD8\n                      <jats:sup>+<\/jats:sup>\n                      T cell proportion. Mechanistically, PCAT6 interacted with IGF2BP1 in the cytoplasm and promoted IGF2BP1\u2010mediated stabilization of PD\u2010L1 mRNA. Rescue experiments confirmed that PCAT6 required IGF2BP1 to sustain PD\u2010L1 expression and mRNA stability.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>PCAT6 facilitates TNBC immune escape by enhancing PD\u2010L1 mRNA stability through IGF2BP1, identifying the PCAT6\/IGF2BP1\/PD\u2010L1 axis as a potential therapeutic target for breast cancer immunotherapy.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jgm.70105","type":"journal-article","created":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T06:03:22Z","timestamp":1786514602000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["PCAT6 Regulates IGF2BP1\/PD\u2010L1 to Promote Immune Escape in Breast Cancer"],"prefix":"10.1002","volume":"28","author":[{"given":"Dingping","family":"Sun","sequence":"first","affiliation":[{"name":"Department of Surgical Oncology Gansu Provincial Hospital of Traditional Chinese Medicine  Lanzhou Gansu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanglong","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Breast Surgery Xigu District Maternal and Child Health Care Hospital  Lanzhou Gansu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Surgical Oncology Gansu Provincial Hospital of Traditional Chinese Medicine  Lanzhou Gansu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liping","family":"Gu","sequence":"additional","affiliation":[{"name":"Department of Pathology Gansu Provincial Hospital of Traditional Chinese Medicine  Lanzhou Gansu 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