{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T05:05:32Z","timestamp":1790139932231,"version":"4.0.1"},"reference-count":37,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T00:00:00Z","timestamp":1789948800000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82172848"],"award-info":[{"award-number":["82172848"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82473466"],"award-info":[{"award-number":["82473466"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Gene Medicine"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Non\u2010small cell lung cancer (NSCLC) is a leading cause of cancer\u2010related death worldwide, largely owing to its high metastatic potential. Contactin\u20105 (CNTN5), a glycosylphosphatidylinositol\u2010anchored membrane protein, has been implicated in tumor\u2010related processes; however, its role in NSCLC progression remains unclear. This study aimed to investigate the clinical relevance, biological function, and underlying mechanism of CNTN5 in NSCLC.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>CNTN5 expression was evaluated using public databases and clinical NSCLC specimens. Gain\u2010 and loss\u2010of\u2010function assays were performed in NSCLC cell lines. Cell proliferation, migration, and invasion were assessed using CCK\u20108, wound healing, and Transwell assays, respectively. An experimental lung metastasis model was used to determine metastatic capacity in\u00a0vivo. RNA sequencing and enrichment analysis were performed to explore the molecular mechanisms involved.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>CNTN5 was upregulated in NSCLC tissues and was associated with poor prognosis. CNTN5 overexpression significantly enhanced NSCLC cell migration and invasion, while increased CNTN5 expression promoted lung metastatic colonization in\u00a0vivo. Transcriptomic and protein analyses indicated that CNTN5 was associated with alterations in Hippo signaling pathway. In addition, CNTN5 increased YAP1 expression and promoted its nuclear translocation, while YAP1 silencing partially reversed the pro\u2010migratory and pro\u2010invasive effects induced by CNTN5. Co\u2010immunoprecipitation analyses further supported an interaction between CNTN5 and PTPN13, accompanied by enhanced nuclear localization of YAP1.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>CNTN5 promotes NSCLC progression, particularly invasion and metastasis, and is associated with unfavorable clinical outcomes. Mechanistically, CNTN5 enhances YAP1 nuclear translocation and activates YAP1\u2010associated transcriptional programs, highlighting its potential as a prognostic biomarker and therapeutic target in NSCLC.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jgm.70112","type":"journal-article","created":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T07:54:27Z","timestamp":1789977267000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["CNTN5 Promotes Invasion and Metastasis in Non\u2010Small Cell Lung Cancer by Regulating YAP1 Nuclear Translocation"],"prefix":"10.1002","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-1456-222X","authenticated-orcid":false,"given":"Jiarui","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Thoracic Surgery, Shanghai Pulmonary Hospital, School of Medicine Tongji University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengtao","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery, Shanghai Pulmonary Hospital, School of Medicine Tongji University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanze","family":"Yin","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery, Shanghai Pulmonary Hospital, School of Medicine Tongji University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ao","family":"Zeng","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery, Shanghai Pulmonary Hospital, School of Medicine Tongji University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keyi","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery, Shanghai Pulmonary Hospital, School of Medicine Tongji University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhilong","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery, Shanghai Pulmonary Hospital, School of Medicine Tongji University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4316-4243","authenticated-orcid":false,"given":"Jie","family":"Dai","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery, Shanghai Pulmonary Hospital, School of Medicine Tongji University  Shanghai 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