{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T00:37:03Z","timestamp":1790469423173,"version":"4.1.0"},"reference-count":54,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,11,25]],"date-time":"2005-11-25T00:00:00Z","timestamp":1132876800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Gene Medicine"],"published-print":{"date-parts":[[2006,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Lentiviral vectors, due to their capacity to transduce non\u2010dividing cells, have become precious and worldwide used gene transfer systems. Their ability to efficiently and stably transduce dendritic cells (DCs) has led to their successful use as vaccination vectors for eliciting strong, specific and protective cellular immune responses mostly in anti\u2010tumoral but also in anti\u2010viral applications. However, the ability of lentiviral vectors to elicit an antibody\u2010based protective immunity has, to date, not been evaluated. In the present study, we evaluated the potential of a lentiviral vector\u2010based vaccine to elicit humoral immunity against West Nile virus (WNV). WNV is a mosquito\u2010borne flavivirus that emerged in North America and causes encephalitis in humans, birds and horses. Neutralizing anti\u2010WNV antibodies have been shown to be crucial for protection against WNV encephalitis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The ability of lentiviral vector TRIP\/sE<jats:sub>WNV<\/jats:sub>, expressing the secreted soluble form of the envelope E\u2010glycoprotein (sE<jats:sub>WNV<\/jats:sub>) from the highly virulent IS\u201098\u2010ST1 strain of WNV, to induce a specific humoral response and protection against WNV infection was assessed in a mouse model of WNV encephalitis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Remarkably, a single immunization with a minute dose of TRIP\/sE<jats:sub>WNV<\/jats:sub> was efficient at eliciting a long\u2010lasting, protective and sterilizing humoral immunity, only 1 week after priming.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study broadens the applicability of lentiviral vectors as efficient non\u2010replicating vaccines against pathogens for which a neutralizing humoral response is one active arm of the protective immunity. The TRIP\/sE<jats:sub>WNV<\/jats:sub> lentiviral vector appears to be a promising tool for veterinary vaccination against zoonotic WNV. Copyright \u00a9 2005 John Wiley &amp; Sons, Ltd.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jgm.837","type":"journal-article","created":{"date-parts":[[2005,11,25]],"date-time":"2005-11-25T10:47:20Z","timestamp":1132915640000},"page":"265-274","source":"Crossref","is-referenced-by-count":69,"title":["A single immunization with a minute dose of a lentiviral vector\u2010based vaccine is highly effective at eliciting protective humoral immunity against West Nile virus"],"prefix":"10.1002","volume":"8","author":[{"given":"Maria 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