{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T04:53:29Z","timestamp":1791348809209,"version":"4.3.1"},"reference-count":49,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,11,21]],"date-time":"2005-11-21T00:00:00Z","timestamp":1132531200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Gene Medicine"],"published-print":{"date-parts":[[2006,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      We have developed minimal non\u2010primate lentiviral vectors based on the equine infectious anaemia virus (EIAV). We evaluated the\n                      <jats:italic>in vivo<\/jats:italic>\n                      expression profiles of these vectors delivered regionally to ocular tissues to define their potential utility in ocular gene therapy.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      EIAV vectors pseudotyped with VSV\u2010G or rabies\u2010G envelope proteins were delivered subretinally, intravitreally or into the anterior chambers (intracameral administration) in mice. Reporter gene (\n                      <jats:italic>eGFP<\/jats:italic>\n                      ) expression was analysed using\n                      <jats:italic>in vivo<\/jats:italic>\n                      retinal imaging or histological examination of eyes and brains at intervals between 3 days and 16 months. We investigated the effects of vector titre, pseudotype, genome configuration, site of intraocular administration, intentional retinal trauma and the degree of retinal maturation on the spatial and temporal expression profiles of these vectors.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Subretinal vector delivery resulted in efficient and stable transduction of retinal pigment epithelial (RPE) cells and variable transduction of photoreceptors up to 16 months post\u2010injection. Retinal trauma facilitated the local transduction of neurosensory retinal cells. Intracameral administration of VSV\u2010G\u2010 but not rabies\u2010G\u2010pseudotyped vectors produced stable\n                      <jats:italic>eGFP<\/jats:italic>\n                      expression in corneal endothelial cells and trabecular meshwork.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The cellular tropism and expression kinetics of optimised EIAV vectors after intraocular administration make them attractive vehicles for delivering therapeutic genes in the management of inherited and acquired retinal and anterior segment disorders. Copyright \u00a9 2005 John Wiley &amp; Sons, Ltd.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jgm.845","type":"journal-article","created":{"date-parts":[[2005,11,21]],"date-time":"2005-11-21T07:46:24Z","timestamp":1132559184000},"page":"275-285","source":"Crossref","is-referenced-by-count":71,"title":["Stable and efficient intraocular gene transfer using pseudotyped EIAV lentiviral vectors"],"prefix":"10.1002","volume":"8","author":[{"given":"K. 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