{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T22:06:04Z","timestamp":1779401164026,"version":"3.53.1"},"reference-count":21,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2006,4,24]],"date-time":"2006-04-24T00:00:00Z","timestamp":1145836800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Gene Medicine"],"published-print":{"date-parts":[[2006,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Conventional strategies of gene therapy using viral vectors result in suboptimal localization and potentially dangerous distal spread of vector. We hypothesized that localized delivery of adenoviral gene vectors could be achieved from a polyurethane (PU) film through a mechanism involving anti\u2010viral antibody tethering.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>PU films were formulated with a collagen coating. Anti\u2010adenoviral monoclonal antibodies were covalently bound to the collagen surface. These antibodies enabled tethering of replication\u2010defective adenoviruses [Ad\u2010GFP (encoding green fluorescent protein)] through highly specific antigen\u2010antibody affinity. The binding stability and <jats:italic>in vitro<\/jats:italic> delivery of virus bound on PU films were investigated. Cell culture studies with rat arterial smooth muscle cells (A10) assessed transduction on or near the PU matrix. <jats:italic>In vivo<\/jats:italic> experiments with collagen\u2010coated PU films investigated atrial epicardial implant and subdermal implant models in Yorkshire swine.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We report for the first time successful PU film\u2010based gene delivery using antibody\u2010tethered adenovirus encoding the green fluorescent protein (GFP), demonstrating efficient and highly localized gene delivery to arterial smooth muscle cells in cell culture and pig implant. In comparison, direct injections of viral vectors into subcutaneous sites gave sparse, needle\u2010track\u2010oriented GFP expression patterns.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We conclude that PU film is a suitable platform for a localizable viral vector delivery system that also prevents systemic spread of vector. Gene delivery using PU film\u2010based anti\u2010viral antibody tethering of vectors should be suitable for a wide array of single or multiple therapeutic gene strategies, and for further device\u2010based gene delivery therapeutic strategies. Copyright \u00a9 2006 John Wiley &amp; Sons, Ltd.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jgm.912","type":"journal-article","created":{"date-parts":[[2006,4,24]],"date-time":"2006-04-24T10:21:00Z","timestamp":1145874060000},"page":"690-698","source":"Crossref","is-referenced-by-count":19,"title":["Immobilization of gene vectors on polyurethane surfaces using a monoclonal antibody for localized gene delivery"],"prefix":"10.1002","volume":"8","author":[{"given":"Lin","family":"Mei","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xu","family":"Jin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cunxian","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manyan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. 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