{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T02:03:15Z","timestamp":1771034595920,"version":"3.50.1"},"reference-count":34,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,9,12]],"date-time":"2024-09-12T00:00:00Z","timestamp":1726099200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["eJHaem"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Variants of uncertain significance (VUS) are commonly reported in cancer with the widespread adoption of diagnostic massive parallel sequencing. The rate of reclassification of VUS in patients with haematological malignancy is not known and we evaluated this retrospectively. We also investigated whether re\u2010evaluating VUS in 12\u201324 months or greater than 24 months post\u2010initial classification was significant.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method<\/jats:title><jats:p>A retrospective audit of patients with haematological malignancies referred to the Molecular Medicine Department at the John Hunter Hospital in Newcastle, Australia between September 2018 and December 2021. Data was analysed for VUS, which was then re\u2010analysed in standard software using current somatic variant guidelines. Proportions of VUS at baseline were compared to post\u2010re\u2010analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The most common diagnoses in the patient cohort (<jats:italic>n<\/jats:italic>\u00a0=\u00a0944) were acute myelogenous leukaemia (41%), myelodysplastic syndrome (31%), and chronic myelomonocytic leukaemia (7%). A total of 210 VUS were re\u2010analysed. The most common VUS were in the TET2 (20%), RUNX1 (10%) and DNMT3A (9%) genes. A total of 103 were re\u2010analysed at 24\u201339 months post\u2010initial classification and 107 variants were re\u2010analysed between 12 and 24 months post\u2010initial classification. Of these, 33 (16%) of VUS were re\u2010classified at 24\u201339 months and 12 (11%) were re\u2010classified at 12\u201324 months post\u2010initial classification. The most common variants that were re\u2010classified in both groups were CSF3R (32%), TET2 (29%), ASXL1 (11%) and ZRSR2 (11%).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study on reclassification of VUS in blood cancers demonstrated that one in seven VUS were re\u2010classified 12 months post initial classification. This can inform practice guidelines and potentially impact the prognosis, diagnosis and treatment of haematological malignancies.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jha2.1002","type":"journal-article","created":{"date-parts":[[2024,9,12]],"date-time":"2024-09-12T15:37:42Z","timestamp":1726155462000},"page":"957-963","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Certainty in uncertainty: Determining the rate and reasons for reclassification of variants of uncertain significance in haematological malignancies"],"prefix":"10.1002","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8069-090X","authenticated-orcid":false,"given":"Anoop K.","family":"Enjeti","sequence":"first","affiliation":[{"name":"Department of Molecular Medicine NSW Health Pathology, John Hunter Hospital  Waratah Australia"},{"name":"Department of Haematology Calvary Mater Newcastle  Waratah Australia"},{"name":"Precision Medicine 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