{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T12:23:05Z","timestamp":1764937385994,"version":"3.40.5"},"reference-count":20,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,3,8]],"date-time":"2022-03-08T00:00:00Z","timestamp":1646697600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["eJHaem"],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Since paroxysmal nocturnal haemoglobinuria (PNH) was first described in 1881, the diagnosis and follow\u2010up patients diagnosed with the illness has remained an area of concern, with several different techniques of varying sensitivity having been described in the literature for both the diagnosis and monitoring treatment of the disease. PNH is a rare and life\u2010threatening disease that manifests symptoms of haemolytic anaemia. Hence, a quick and reliable technique for precise diagnosis would be crucial. PNH patients who have previously been diagnosed with aplastic anaemia or myelodysplastic syndrome carry small PNH clones and for more than a century traditional method with low sensitivity was used for such patients. In 2010, the International Clinical Cytometry Society described a highly sensitive method for detection and quantification of different types of PNH clones using multi\u2010colour flow cytometry. In this method, a three\u2010colour flow cytometer is essential to detect PNH affected cells amongst monocytes and granulocytes. This started a new era in the diagnosis of patients who carry small clones of PNH cells. Before this, flow cytometric analysis was used only for detection of PNH cells amongst erythrocytes. By using flow cytometry instruments with more light sources, the sensitivity of detection and quantification of PNH clones would be augmented. However, standardisation and crosstalk compensation would be the most concerning issue. For the first time in Iran, we set up and standardised multi\u2010colour flow cytometry technique to detect PNH cells in erythrocytes and leukocytes at Payvand medical laboratory.<\/jats:p>","DOI":"10.1002\/jha2.410","type":"journal-article","created":{"date-parts":[[2022,3,9]],"date-time":"2022-03-09T06:42:14Z","timestamp":1646808134000},"page":"335-340","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Paroxysmal nocturnal haemoglobinuria, diagnosis and haematological findings, first report from Iran, model for developing countries"],"prefix":"10.1002","volume":"3","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9251-7239","authenticated-orcid":false,"given":"Mohammadali","family":"Jahangirpour","sequence":"first","affiliation":[{"name":"Portiuncula University Hospital Ballinasloe Co. Galway Republic of Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amirali","family":"Vahedi","sequence":"additional","affiliation":[{"name":"Payvand Clinical and Specialty Laboratory Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hamed","family":"Baghdadi","sequence":"additional","affiliation":[{"name":"Payvand Clinical and Specialty Laboratory Tehran Iran"},{"name":"Department of Hematology Faculty of Medical Sciences Tarbiat Modares University Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tahereh","family":"Madani","sequence":"additional","affiliation":[{"name":"Payvand Clinical and Specialty Laboratory Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ali","family":"Behvarmanesh","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Centre de Recherche en Biologie Structurale McGill University Montreal Quebec 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