{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T20:58:23Z","timestamp":1770843503539,"version":"3.50.1"},"reference-count":46,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,6,16]],"date-time":"2022-06-16T00:00:00Z","timestamp":1655337600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["eJHaem"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Burkitt lymphoma (BL) represents the most aggressive B\u2010cell\u2010lymphoma. Beside the hallmark of <jats:italic>IG\u2010MYC<\/jats:italic>\u2010translocation, surface B\u2010cell receptor (BCR) is expressed, and mutations in the BCR pathway are frequent. Coincidental infections in endemic BL, and specific extra\u2010nodal sites suggest antigenic triggers. To explore this hypothesis, BCRs of BL cell lines and cases were screened for reactivities against a panel of bacterial lysates, lysates of <jats:italic>Plasmodium falciparum<\/jats:italic>, a custom\u2010made virome array and against self\u2010antigens, including post\u2010translationally modified antigens.<\/jats:p><jats:p>An atypically modified, SUMOylated isoform of Bystin, that is, SUMO1\u2010BYSL was identified as the antigen of the BCR of cell line CA46. SUMO1\u2010BYSL was exclusively expressed in CA46 cells with K139 as site of the SUMOylation. Secondly, an atypically acetylated isoform of HSP40 was identified as the antigen of the BCR of cell line BL41. K104 and K179 were the sites of immunogenic acetylation, and the acetylated HSP40 isoform was solely present in BL41 cells. Functionally, addition of SUMO1\u2010BYSL and acetylated HSP40 induced BCR pathway activation in CA46 and BL41 cells, respectively. Accordingly, SUMO1\u2010BYSL\u2010ETA\u2019 immunotoxin, produced by a two\u2010step intein\u2010based conjugation, led to the specific killing of CA46 cells. Autoantibodies directed against SUMO1\u2010BYSL were found in 3 of 14 (21.4%), and autoantibodies against acetylated HSP40 in 1\/14(7.1%) patients with sporadic Burkitt\u2010lymphoma. No reactivities against antigens of the infectious agent spectrum could be observed.<\/jats:p><jats:p>These results indicate a pathogenic role of autoreactivity evoked by immunogenic post\u2010translational modifications in a subgroup of sporadic BL including two EBV\u2010negative BL cell lines.<\/jats:p>","DOI":"10.1002\/jha2.475","type":"journal-article","created":{"date-parts":[[2022,6,16]],"date-time":"2022-06-16T07:13:17Z","timestamp":1655363597000},"page":"739-747","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["B\u2010cell receptors of EBV\u2010negative Burkitt lymphoma bind modified isoforms of autoantigens"],"prefix":"10.1002","volume":"3","author":[{"given":"Theresa","family":"Bock","sequence":"first","affiliation":[{"name":"Department of Internal Medicine I and Jos\u00e9 Carreras Center for Immuno\u2010 and Gene Therapy Saarland University Medical School  Homburg\/Saar 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Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan","family":"Lohse","sequence":"additional","affiliation":[{"name":"Institute of Virology University of Saarland  Homburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thorsten","family":"Pfuhl","sequence":"additional","affiliation":[{"name":"Institute of Virology University of Saarland  Homburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rabea","family":"Wagener","sequence":"additional","affiliation":[{"name":"Institute of Human Genetics Ulm University and Ulm University Medical Center  Ulm Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sigrun","family":"Smola","sequence":"additional","affiliation":[{"name":"Institute of Virology University of Saarland  Homburg Germany"},{"name":"Helmholtz Institute for Pharmaceutical Research Saarland (HIPS)  Saarbr\u00fccken Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S\u00f6ren L.","family":"Becker","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology and Hygiene Saarland University  Homburg\/Saar Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rainer Maria","family":"Bohle","sequence":"additional","affiliation":[{"name":"Institute of Pathology Saarland University Medical School  Homburg\/Saar Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lorenz","family":"Tr\u00fcmper","sequence":"additional","affiliation":[{"name":"Department of Hematology and Oncology Georg August University G\u00f6ttingen  G\u00f6ttingen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reiner","family":"Siebert","sequence":"additional","affiliation":[{"name":"Institute of Human Genetics Ulm University and Ulm University Medical Center  Ulm Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin\u2010Leo","family":"Hansmann","sequence":"additional","affiliation":[{"name":"Dr. Senckenberg Institute of Pathology Goethe University Hospital of Frankfurt a. Main  Frankfurt a. Main Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Pfreundschuh","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine I and Jos\u00e9 Carreras Center for Immuno\u2010 and Gene Therapy Saarland University Medical School  Homburg\/Saar Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans G.","family":"Drexler","sequence":"additional","affiliation":[{"name":"Faculty of Life sciences Technical University of Braunschweig  Braunschweig Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Markus","family":"Hoth","sequence":"additional","affiliation":[{"name":"Center for Integrative Physiology and Molecular Medicine (CIPMM) School of Medicine  Homburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Boris","family":"Kubuschok","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine II Augsburg University Medical Center  Augsburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Klaus","family":"Roemer","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine I and Jos\u00e9 Carreras Center for Immuno\u2010 and Gene Therapy Saarland University Medical School  Homburg\/Saar Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7559-5342","authenticated-orcid":false,"given":"Klaus\u2010Dieter","family":"Preuss","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine I and Jos\u00e9 Carreras Center for Immuno\u2010 and Gene Therapy Saarland University Medical School  Homburg\/Saar Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sylvia","family":"Hartmann","sequence":"additional","affiliation":[{"name":"Dr. Senckenberg Institute of Pathology Goethe University Hospital of Frankfurt a. 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Main Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8167-2989","authenticated-orcid":false,"given":"Lorenz","family":"Thurner","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine I and Jos\u00e9 Carreras Center for Immuno\u2010 and Gene Therapy Saarland University Medical School  Homburg\/Saar Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,6,16]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2016-01-643569"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2015.07.019"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/10428194.2021.1907374"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.79.24.7837"},{"key":"e_1_2_10_6_1","article-title":"A recurrent 11q aberration pattern characterizes a subset of MYC\u2010negative 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Saarland Germany: Homburg\/Saar; 2021."},{"key":"e_1_2_10_47_1","article-title":"Identification of the atypically modified autoantigen Ars2 as the target of B\u2010cell receptors from activated B cell\u2010type diffuse large B\u2010cell lymphoma","author":"Thurner L","journal-title":"Haematologica"}],"container-title":["eJHaem"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jha2.475","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jha2.475","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jha2.475","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T03:05:26Z","timestamp":1692587126000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jha2.475"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6,16]]},"references-count":46,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2022,8]]}},"alternative-id":["10.1002\/jha2.475"],"URL":"https:\/\/doi.org\/10.1002\/jha2.475","archive":["Portico"],"relation":{},"ISSN":["2688-6146","2688-6146"],"issn-type":[{"value":"2688-6146","type":"print"},{"value":"2688-6146","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,6,16]]},"assertion":[{"value":"2022-03-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-05-01","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-06-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}