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The original transcriptomic approach of reverse transcriptase multiplex ligation\u2010dependent probe amplification (RT\u2010MLPA) was applied here to explore the expression of 24 genes of interest in MCL at diagnosis (21 LN and 15\u00a0BM) or relapse (18 LN). This allowed us to identify that at baseline, samples from MCL patients with an aggressive morphology (i.e. blastoid or pleomorphic) or a high proliferative profile, displayed significantly higher monocyte\/macrophage\u2010associated transcripts (<jats:italic>CD14<\/jats:italic> and <jats:italic>CD163)<\/jats:italic> in LN and BM. Regarding T\u2010cells, aggressive MCL forms had significantly lower amounts of LN <jats:italic>CD3E<\/jats:italic> transcripts, yet an increased expression of cytotoxic markers in LN (<jats:italic>CD8<\/jats:italic>) and BM (<jats:italic>CD94<\/jats:italic>). A very high\u2010risk group with early treatment resistance displayed, at diagnosis, high proliferation (<jats:italic>KI67<\/jats:italic>) and high macrophages and cytotoxic transcript levels. Post\u2010immunochemotherapy relapsed samples revealed lower levels of T\u2010 and natural killer\u2010cells markers, while monocyte\/macrophage markers remained similar to diagnosis. This study suggests that rapid analysis of MCL microenvironment transcriptome signatures by RT\u2010MLPA could allow for an early distinction of patient subgroups candidates for adapted treatment strategies.<\/jats:p>","DOI":"10.1002\/jha2.549","type":"journal-article","created":{"date-parts":[[2022,10,14]],"date-time":"2022-10-14T05:44:11Z","timestamp":1665726251000},"page":"1165-1171","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Aggressive, early resistant and relapsed mantle cell lymphoma distinct extrinsic microenvironment highlighted by transcriptome analysis"],"prefix":"10.1002","volume":"3","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0095-6999","authenticated-orcid":false,"given":"Yannick","family":"Le Bris","sequence":"first","affiliation":[{"name":"Hematology Biology Nantes University Hospital  Nantes France"},{"name":"CRCINA INSERM CNRS Universit\u00e9 d'Angers Universit\u00e9 de Nantes  Nantes 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