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While contribution of RNA processing alteration has been linked to other hematological malignancies, its contribution in pediatric T\u2010ALL may provide new insights. Almost all human genes express more than one alternative splice isoform. Thus, gene modulation producing a diverse repertoire of the transcriptome and proteome have become a significant molecular marker of cancer and a potential therapeutic vulnerability. To study this, we performed RNA\u2010sequencing analysis on patient\u2010derived samples followed by splice isoform\u2010specific PCR. We uncovered a distinct RNA splice isoform expression pattern characteristic for relapse samples compared to the leukemia samples from the time of diagnosis. We also identified deregulated splicing and apoptosis pathways specific for relapse T\u2010ALL. Moreover, patients with T\u2010ALL displayed pro\u2010survival splice isoform switching favoring pro\u2010survival isoforms compared to normal healthy stem cells. 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