{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T17:24:33Z","timestamp":1786123473745,"version":"build-2736575974"},"reference-count":54,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T00:00:00Z","timestamp":1768780800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T00:00:00Z","timestamp":1768780800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004881","name":"Instituto Mexicano del Seguro Social","doi-asserted-by":"publisher","award":["R\u20102021\u2010785\u2010028"],"award-info":[{"award-number":["R\u20102021\u2010785\u2010028"]}],"id":[{"id":"10.13039\/501100004881","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["eJHaem"],"published-print":{"date-parts":[[2026,2]]},"abstract":"ABSTRACT<\/jats:title>\n \n Introduction<\/jats:title>\n In Mexico, the 5\u2010year overall survival (OS) rate for pediatric acute lymphoblastic leukemia (ALL) ranges from 45% to 85%, markedly lower than the \u223c90% reported in high\u2010income countries, where cytogenomic testing is essential for accurate risk stratification and therapeutic decision\u2010making. The few available data for Mexican cohorts derive from studies conducted in Mexico City using conventional karyotyping, DNA index analysis, and RT\u2010PCR targeting only four gene fusions. Broader cytogenomic characterization is needed to identify additional prognostic alterations.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n We analyzed 170 pediatric ALL cases (150 B\u2010Cell lineage, 10 T\u2010Cell lineage, and 10 mixed phenotype) using fluorescence in situ hybridization (FISH) with a panel of 11 probe sets targeting recurrent cytogenomic abnormalities. All patients were treated according to the Total XV protocol.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Among 150 B\u2010Cell ALL cases, recurrent cytogenomic abnormalities included ETV6\n ::RUNX1<\/jats:italic>\n (\n n<\/jats:italic>\n \u00a0=\u00a019),\n TCF3::PBX1<\/jats:italic>\n (\n n<\/jats:italic>\n \u00a0=\u00a07),\n BCR::ABL1<\/jats:italic>\n (\n n<\/jats:italic>\n \u00a0=\u00a05),\n KMT2A<\/jats:italic>\n ::V (\n n<\/jats:italic>\n \u00a0=\u00a010),\n IGH<\/jats:italic>\n ::V (\n n<\/jats:italic>\n \u00a0=\u00a07), V::\n CRLF2<\/jats:italic>\n (\n n<\/jats:italic>\n \u00a0=\u00a011), iAMP21 (\n n<\/jats:italic>\n \u00a0=\u00a08), and deletions involving\n CDKN2A\/B<\/jats:italic>\n (\n n<\/jats:italic>\n = 38),\n TP53<\/jats:italic>\n (\n n<\/jats:italic>\n = 7),\n RB1<\/jats:italic>\n (8),\n ATM<\/jats:italic>\n (\n n<\/jats:italic>\n = 1), and\n ETV6<\/jats:italic>\n (\n n<\/jats:italic>\n = 15). Hypodiploidy (\n n<\/jats:italic>\n \u00a0=\u00a02), high\u2010hyperdiploidy (\n n<\/jats:italic>\n \u00a0=\u00a038), low\u2010hyperdiploidy (\n n<\/jats:italic>\n \u00a0=\u00a016), and 1q gain (\n n<\/jats:italic>\n \u00a0=\u00a014) were also identified.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n Our findings reveal a cytogenomic landscape characterized by a predominance of high\u2010risk abnormalities such as iAMP21 and\n KMT2A<\/jats:italic>\n ::V, together with a lower frequency of low\u2010risk alterations like\n ETV6::RUNX1<\/jats:italic>\n . The frequent coexistence of secondary abnormalities further supports the relevance of comprehensive cytogenomic profiling for accurate risk assessment. The high diagnostic coverage and rapid turnaround of the FISH\u2010based approach underscore its value as a reliable and efficient diagnostic tool in newly diagnosed ALL.\n <\/jats:p>\n <\/jats:sec>\n \n Trial Registration<\/jats:title>\n The authors have confirmed clinical trial registration is not needed for this submission<\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/jha2.70220","type":"journal-article","created":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T13:22:25Z","timestamp":1768828945000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Cytogenomic Abnormalities in Children With Acute Lymphoblastic Leukemia From Western Mexico: A Single\u2010Center Fluorescence In Situ Hybridization\u2010Based Study"],"prefix":"10.1002","volume":"7","author":[{"given":"Rosa Mar\u00eda Gonz\u00e1lez","family":"Arreola","sequence":"first","affiliation":[{"name":"Doctorado en Gen\u00e9tica Humana Centro Universitario en Ciencias de la Salud Universidad de Guadalajara Guadalajara Jalisco Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Teresa Maga\u00f1a","family":"Torres","sequence":"additional","affiliation":[{"name":"Divisi\u00f3n de Gen\u00e9tica Centro de Investigaci\u00f3n Biom\u00e9dica de Occidente Instituto Mexicano del Seguro Social Guadalajara Jalisco Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ma. Guadalupe Dom\u00ednguez","family":"Quezada","sequence":"additional","affiliation":[{"name":"Divisi\u00f3n de Gen\u00e9tica Centro de Investigaci\u00f3n Biom\u00e9dica de Occidente Instituto Mexicano del Seguro Social Guadalajara Jalisco Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janet Margarita Soto","family":"Padilla","sequence":"additional","affiliation":[{"name":"Servicio de Hematolog\u00eda UMAE\u2010Hospital de Pediatr\u00eda Centro M\u00e9dico Nacional de Occidente Instituto Mexicano del Seguro Social Guadalajara Jalisco Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Luis Toro","family":"Castro","sequence":"additional","affiliation":[{"name":"Servicio de Hematolog\u00eda UMAE\u2010Hospital de Pediatr\u00eda Centro M\u00e9dico Nacional de Occidente Instituto Mexicano del Seguro Social Guadalajara Jalisco Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beatriz Kazuko De la Herr\u00e1n","family":"Arita","sequence":"additional","affiliation":[{"name":"Servicio de Hematolog\u00eda UMAE\u2010Hospital de Pediatr\u00eda Centro M\u00e9dico Nacional de Occidente Instituto Mexicano del Seguro Social Guadalajara Jalisco Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alicia Guti\u00e9rrez","family":"M\u00e9ndez","sequence":"additional","affiliation":[{"name":"Servicio de Hematolog\u00eda UMAE\u2010Hospital de Pediatr\u00eda Centro M\u00e9dico Nacional de Occidente Instituto Mexicano del Seguro Social Guadalajara Jalisco Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hugo Antonio Romo","family":"Rubio","sequence":"additional","affiliation":[{"name":"Servicio de Hematolog\u00eda UMAE\u2010Hospital de Pediatr\u00eda Centro M\u00e9dico Nacional de Occidente Instituto Mexicano del Seguro Social Guadalajara Jalisco Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan Ram\u00f3n 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