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With its adaptable throughput and variable read lengths, Oxford Nanopore thirdgeneration\u00a0sequencing now holds promise for clonotyping. This study analyzed CD138+ plasma\u2010cell DNA from eight multiple myeloma patients, comparing clonotyping performance between Nanopore sequencing, Illumina MiSeq, and Ion Torrent S5. We demonstrated clonotype consistency across platforms through Smith\u2010Waterman local alignment of nanopore reads. The mean clonal percentage of IGH V and J gene usage in the CD138+ cells was 69% for Nanopore, 67% for S5, and 76% for MiSeq. When aligned with known clonotypes, clonal cells averaged a 91% similarity, exceeding 85%. In summary, Nanopore sequencing, with its capacity for generating millions of high\u2010quality reads, proves effective for detecting clonal IGH rearrangements. This versatile platform offers the potential for measuring residual disease down to a sensitivity level of 10<jats:sup>\u20106<\/jats:sup> at a lower cost, marking a significant advancement in clonotyping techniques.<\/jats:p>","DOI":"10.1002\/jha2.815","type":"journal-article","created":{"date-parts":[[2023,11,25]],"date-time":"2023-11-25T01:44:47Z","timestamp":1700876687000},"page":"290-293","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["The potential of 3rd\u2010generation nanopore sequencing for B\u2010cell clonotyping in lymphoproliferative disorders"],"prefix":"10.1002","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3083-4850","authenticated-orcid":false,"given":"Marcus H.","family":"Hansen","sequence":"first","affiliation":[{"name":"Haematology\u2010Pathology Research Laboratory, Research Unit of Haematology Department of Haematology, and Research Unit of Pathology Department of Pathology 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