{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,12]],"date-time":"2026-11-12T14:30:23Z","timestamp":1794493823768,"version":"4.9.1"},"reference-count":34,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2022,12,29]],"date-time":"2022-12-29T00:00:00Z","timestamp":1672272000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["20K08276"],"award-info":[{"award-number":["20K08276"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Hepato Biliary Pancreat"],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Primary sclerosing cholangitis (PSC) is a chronic inflammatory disease of unknown etiology that affects the intra\u2010 and extrahepatic bile ducts. The present study examined the utility of a bile proteome analysis using a high\u2010sensitivity mass spectrometer to comprehensively screen for novel PSC biomarkers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Bile endoscopically collected from patients with PSC, common bile duct stones, and biliary tract cancer were subjected to high\u2010precision liquid chromatography\/mass spectrometry. Some of the proteins specifically up\u2010regulated in the bile of the PSC group were re\u2010examined by an enzyme\u2010linked immunosorbent assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 8094 proteins were successfully identified and 332 were specifically up\u2010regulated in the PSC group. The bioinformatics analysis showed that proteins involved in the proliferation and activation of diverse inflammatory cells were up\u2010regulated in the PSC group. A receiver operating characteristic curve analysis showed good area under the curve values for interleukin\u20108 and annexin A1 (ANXA1) (0.836 and 0.914, respectively). Immunostaining for ANXA1 revealed its strong expression in inflammatory cells infiltrating the peripheral biliary tract in PSC livers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>A bile proteome analysis is a useful tool for elucidating the pathogenesis of PSC and developing new diagnostic approaches. Therefore, ANXA1 has potential as a bile biomarker for PSC.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jhbp.1299","type":"journal-article","created":{"date-parts":[[2022,12,18]],"date-time":"2022-12-18T05:09:15Z","timestamp":1671340155000},"page":"914-923","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Bile proteome analysis by <scp>high\u2010precision<\/scp> mass spectrometry to examine novel biomarkers of primary sclerosing cholangitis"],"prefix":"10.1002","volume":"30","author":[{"given":"Motoyasu","family":"Kan","sequence":"first","affiliation":[{"name":"Department of Gastroenterology, Graduate School of Medicine Chiba University  Chiba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7179-0765","authenticated-orcid":false,"given":"Tetsuhiro","family":"Chiba","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Graduate School of Medicine Chiba University  Chiba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryo","family":"Konno","sequence":"additional","affiliation":[{"name":"Department of Applied Genomics Kazusa DNA Research Institute  Kisarazu Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yusuke","family":"Kouchi","sequence":"additional","affiliation":[{"name":"Department of Molecular Pathology, Graduate School of Medicine Chiba University  Chiba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Mishima","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Graduate School of Medicine Chiba University  Chiba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yusuke","family":"Kawashima","sequence":"additional","affiliation":[{"name":"Department of Applied Genomics Kazusa DNA Research Institute  Kisarazu 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