{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T16:03:42Z","timestamp":1783958622412,"version":"3.55.0"},"reference-count":48,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2014,7,16]],"date-time":"2014-07-16T00:00:00Z","timestamp":1405468800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Hepato Biliary Pancreat"],"published-print":{"date-parts":[[2014,10]]},"abstract":"Abstract<\/jats:title>Background<\/jats:title>We investigated the aberrant expression of the STAT<\/jats:styled-content> family in humans and liver fluke (O<\/jats:styled-content>pisthorchis viverrini<\/jats:italic>, O<\/jats:styled-content>v)\u2010induced hamster cholangiocarcinoma (CCA<\/jats:styled-content>) tissues.<\/jats:p><\/jats:sec>Methods<\/jats:title>The expression and phosphorylation of STAT1<\/jats:styled-content>, STAT2<\/jats:styled-content>, STAT3<\/jats:styled-content>, STAT4<\/jats:styled-content>, STAT5a<\/jats:styled-content>, STAT5b<\/jats:styled-content> and STAT6<\/jats:styled-content> in human hamster CCA<\/jats:styled-content> tissues were immunohistochemistry\u2010profiled. Localizations of STAT5<\/jats:styled-content> in macrophages and lipopolysaccharide (LPS)<\/jats:styled-content>\u2010induced macrophage\u2010conditioned media mediated STAT3<\/jats:styled-content> activation in CCA<\/jats:styled-content> cells were demonstrated.<\/jats:p><\/jats:sec>Results<\/jats:title>The expressions of STAT<\/jats:styled-content> 1\u20134 and 6 were detected in the cytoplasm of hyperplastic bile ducts and tumor cells, whereas STAT5a<\/jats:styled-content> and STAT5b<\/jats:styled-content> were observed in macrophages and connective tissues surrounding tumor, respectively. The expressions of STAT3<\/jats:styled-content> and STAT5b<\/jats:styled-content> were significantly observed in tumors with a poorer histological differentiation. STAT3<\/jats:styled-content> expression was significantly associated with shorter survival of CCA<\/jats:styled-content> patients and was predominately activated in CCA<\/jats:styled-content> cell lines. In the CCA<\/jats:styled-content>\u2010hamsters, STAT<\/jats:styled-content>s expression was gradually increased along the carcinogenesis, especially at 30 days post\u2010infection in which the inflammatory response was markedly observed, showing the correlation between the inflammation and STAT<\/jats:styled-content>s activation. Moreover, LPS<\/jats:styled-content>\u2010induced macrophage\u2010conditioned media could mediate STAT3<\/jats:styled-content> activation in CCA<\/jats:styled-content> cells.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>STAT3<\/jats:styled-content> is the major STAT,<\/jats:styled-content> which plays roles in the inflammation that contributes to CCA<\/jats:styled-content> carcinogenesis and progression and may serve as a marker for a poor prognosis of CCA<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jhbp.131","type":"journal-article","created":{"date-parts":[[2014,7,16]],"date-time":"2014-07-16T10:49:44Z","timestamp":1405507784000},"page":"767-776","source":"Crossref","is-referenced-by-count":40,"title":["STAT<\/scp>s profiling reveals predominantly\u2010activated STAT3<\/scp> in cholangiocarcinoma genesis and progression"],"prefix":"10.1002","volume":"21","author":[{"given":"Hasaya","family":"Dokduang","sequence":"first","affiliation":[{"name":"Department of Biochemistry Faculty of Medicine Khon Kaen University Khon Kaen 40002 Thailand"},{"name":"Liver Fluke and Cholangiocarcinoma Research Center Khon Kaen University Khon Kaen Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anchalee","family":"Techasen","sequence":"additional","affiliation":[{"name":"Center for Research Development of Medical Diagnostic Laboratories Faculty of Associated Medical Science Khon Kaen University Khon Kaen Thailand"},{"name":"Liver Fluke and Cholangiocarcinoma Research Center Khon Kaen University Khon Kaen Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nisana","family":"Namwat","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Faculty of Medicine Khon Kaen University Khon Kaen 40002 Thailand"},{"name":"Liver Fluke and Cholangiocarcinoma Research Center Khon Kaen University Khon Kaen Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Narong","family":"Khuntikeo","sequence":"additional","affiliation":[{"name":"Department of Surgery Faculty of Medicine Khon Kaen University Khon Kaen Thailand"},{"name":"Liver Fluke and Cholangiocarcinoma Research Center Khon Kaen University Khon Kaen Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chawalit","family":"Pairojkul","sequence":"additional","affiliation":[{"name":"Department of Pathology Faculty of Medicine Khon Kaen University Khon Kaen Thailand"},{"name":"Liver Fluke and Cholangiocarcinoma Research Center Khon Kaen University Khon Kaen Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshinori","family":"Murakami","sequence":"additional","affiliation":[{"name":"Division of Molecular Pathology Institute of Medical Science The University of Tokyo Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Watcharin","family":"Loilome","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Faculty of Medicine Khon Kaen University Khon Kaen 40002 Thailand"},{"name":"Liver Fluke and Cholangiocarcinoma Research Center Khon Kaen University Khon Kaen Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Puangrat","family":"Yongvanit","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Faculty of Medicine Khon Kaen University Khon Kaen 40002 Thailand"},{"name":"Liver Fluke and Cholangiocarcinoma Research Center Khon Kaen University Khon Kaen Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,7,16]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(90)90159-C"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1996.55.295"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910590412"},{"issue":"4","key":"e_1_2_8_5_1","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1093\/annonc\/10.suppl_4.S122","article-title":"Mechanisms of biliary carcinogenesis: a pathogenetic multi\u2010stage cascade towards cholangiocarcinoma","volume":"10","author":"Holzinger F","year":"1999","journal-title":"Ann Oncol"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)67530-7"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-8278(02)00297-0"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2004-828889"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrfmmm.2011.10.009"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.niox.2004.08.004"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2003.08.039"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1158\/1055-9965.EPI-07-2717"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2011.06.011"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.25646"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/mc.20094"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.2009.01427.x"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13277-013-0930-9"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1203476"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-437X(98)80015-4"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(00)01624-8"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI0215617"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.13.6478"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1204086"},{"key":"e_1_2_8_24_1","first-page":"115","article-title":"Cytokines released from activated human macrophages induce epithelial mesenchymal transition markers of cholangiocarcinoma cells","volume":"13","author":"Techasen A","year":"2012","journal-title":"Asian Pac J Cancer Prev"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.2806"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.3880409"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.15.4240"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1186\/1749-8090-8-92"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canep.2011.11.007"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-008-0474-z"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.1027"},{"key":"e_1_2_8_32_1","first-page":"597","article-title":"Tissue invasive macrophage density is correlated with prognosis in cholangiocarcinoma","volume":"3","author":"Subimerb C","year":"2010","journal-title":"Mol Med Rep"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/18.5.1367"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2734"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.15.3.1312"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M307774200"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.critrevonc.2005.08.005"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1203486"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm976"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1359-6101(00)00005-8"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.16.4.1595"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.18.5.2545"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.20966"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2006.10.037"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2013.01.001"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2012.617"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.5061"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.23134"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001654"}],"container-title":["Journal of Hepato-Biliary-Pancreatic Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjhbp.131","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jhbp.131","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T16:37:38Z","timestamp":1696523858000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jhbp.131"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,7,16]]},"references-count":48,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2014,10]]}},"alternative-id":["10.1002\/jhbp.131"],"URL":"https:\/\/doi.org\/10.1002\/jhbp.131","archive":["Portico"],"relation":{},"ISSN":["1868-6974","1868-6982"],"issn-type":[{"value":"1868-6974","type":"print"},{"value":"1868-6982","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,7,16]]}}}