{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T03:39:55Z","timestamp":1761709195080},"reference-count":34,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2016,4,4]],"date-time":"2016-04-04T00:00:00Z","timestamp":1459728000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Hepato Biliary Pancreat"],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>We previously reported a model of non\u2010alcoholic fatty liver disease (NAFLD) using spontaneously hypertensive rats (SHRs), fed a choline\u2010deficient (CD) diet for 5\u00a0weeks, that hepatic steatosis but not fibrosis is developed through oxidative stress. To determine the relationship between hypertension and hepatic fibrosis in NAFLD, we examined whether long\u2010term CD diet leads to hepatic fibrosis through oxidative stress.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Eight\u2010week\u2010old male SHR and normotensive Wistar Kyoto rats (WKYs) were fed a CD diet for 5 or 20\u00a0weeks, then liver histology and hepatic expression of genes related to lipid metabolism, fibrosis, and oxidative stress were assessed. Oxidative stress was assessed by hepatic thiobarbituric acid reactive substance (TBARS) levels.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>After 5\u00a0weeks on CD diet, prominent hepatic steatosis and decrease in expression of genes for lipid metabolism were observed in SHRs as compared with WKYs. SHRs on a CD diet demonstrated a downregulated expression of genes for antioxidants, along with significant increases in hepatic TBARS. After 20\u00a0weeks on CD diet, SHRs demonstrated severe liver fibrosis and upregulated expressions of genes for fibrosis when compared with WKY.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Hypertension precipitated hepatic steatosis, and further, acts as an enhancer in NAFLD progression to liver fibrosis through oxidative stress.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jhbp.333","type":"journal-article","created":{"date-parts":[[2016,2,16]],"date-time":"2016-02-16T12:00:09Z","timestamp":1455624009000},"page":"260-269","source":"Crossref","is-referenced-by-count":11,"title":["Enhancing hepatic fibrosis in spontaneously hypertensive rats fed a choline\u2010deficient diet: a follow\u2010up report on long\u2010term effects of oxidative stress in non\u2010alcoholic fatty liver disease"],"prefix":"10.1002","volume":"23","author":[{"given":"Hiroya","family":"Yamamoto","sequence":"first","affiliation":[{"name":"Department of General Internal Medicine Hiroshima 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