{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T09:34:26Z","timestamp":1758706466509},"reference-count":20,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,3,12]],"date-time":"2018-03-12T00:00:00Z","timestamp":1520812800000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["26461046"],"award-info":[{"award-number":["26461046"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007655","name":"Aichi Cancer Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007655","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Hepato Biliary Pancreat"],"published-print":{"date-parts":[[2018,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Fluorescence in\u00a0situ hybridization (<jats:styled-content style=\"fixed-case\">FISH<\/jats:styled-content>) of cytology specimens has been used to diagnose biliary strictures. However, the usefulness of <jats:styled-content style=\"fixed-case\">FISH<\/jats:styled-content> for differentiating between cholangiocarcinoma (<jats:styled-content style=\"fixed-case\">CCA<\/jats:styled-content>) and IgG4\u2010related sclerosing cholangitis (IgG4\u2010<jats:styled-content style=\"fixed-case\">SC<\/jats:styled-content>) has not been evaluated in forceps biopsy specimens.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We retrospectively reviewed 74 specimens obtained by transpapillary forceps biopsy between 2008 and 2015 from 49 consecutive patients with <jats:styled-content style=\"fixed-case\">CCA<\/jats:styled-content> and 25 with IgG4\u2010<jats:styled-content style=\"fixed-case\">SC<\/jats:styled-content>. Specimens were considered positive for malignancy by <jats:styled-content style=\"fixed-case\">FISH<\/jats:styled-content> with UroVysion<jats:sup>\u00ae<\/jats:sup> if at least five cells exhibited polysomy (a gain of two or more in chromosomes 3, 7, or 17).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 27 (55.1%) patients with <jats:styled-content style=\"fixed-case\">CCA<\/jats:styled-content>, but none of the patients with IgG4\u2010<jats:styled-content style=\"fixed-case\">SC<\/jats:styled-content>, were positive for malignancy by <jats:styled-content style=\"fixed-case\">FISH<\/jats:styled-content>. The sensitivity, specificity, positive predictive value, negative predictive value, and accuracy of <jats:styled-content style=\"fixed-case\">FISH<\/jats:styled-content> for the diagnosis of <jats:styled-content style=\"fixed-case\">CCA<\/jats:styled-content> were 55.1%, 100%, 100%, 53.2%, and 70.3%, respectively. The complementary use of <jats:styled-content style=\"fixed-case\">FISH<\/jats:styled-content> increased the sensitivity of hematoxylin\u2010and\u2010eosin (H&amp;E) staining from 69.4% to 77.6%; the specificity was not reduced when either H&amp;E or <jats:styled-content style=\"fixed-case\">FISH<\/jats:styled-content> was positive.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The use of <jats:styled-content style=\"fixed-case\">FISH<\/jats:styled-content> in the analysis of forceps biopsy specimens might be one option to differentiate <jats:styled-content style=\"fixed-case\">CCA<\/jats:styled-content> from IgG4\u2010<jats:styled-content style=\"fixed-case\">SC<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jhbp.536","type":"journal-article","created":{"date-parts":[[2018,1,23]],"date-time":"2018-01-23T15:42:56Z","timestamp":1516722176000},"page":"188-194","source":"Crossref","is-referenced-by-count":13,"title":["Differential diagnosis of cholangiocarcinoma and IgG4\u2010related sclerosing cholangitis by fluorescence in\u00a0situ hybridization using transpapillary forceps biopsy specimens"],"prefix":"10.1002","volume":"25","author":[{"given":"Akihisa","family":"Kato","sequence":"first","affiliation":[{"name":"Department of Gastroenterology and Metabolism Nagoya City University Graduate School of Medical Sciences Nagoya Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Itaru","family":"Naitoh","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Metabolism Nagoya City University Graduate School of Medical Sciences Nagoya Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katsuyuki","family":"Miyabe","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Metabolism Nagoya City University Graduate School of Medical Sciences Nagoya Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuki","family":"Hayashi","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Metabolism Nagoya City University Graduate School of Medical Sciences Nagoya Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiromu","family":"Kondo","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Metabolism Nagoya City University Graduate School of Medical Sciences Nagoya Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michihiro","family":"Yoshida","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Metabolism Nagoya City University Graduate School of Medical Sciences Nagoya Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroyuki","family":"Kato","sequence":"additional","affiliation":[{"name":"Department of Experimental Pathology and Tumor Biology Nagoya City University Graduate School of Medical Sciences 1 Kawasumi Mizuho\u2010cho, Mizuho\u2010ku Nagoya 467\u20108601 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshiya","family":"Kuno","sequence":"additional","affiliation":[{"name":"Department of Experimental Pathology and Tumor Biology Nagoya City University Graduate School of Medical Sciences 1 Kawasumi Mizuho\u2010cho, Mizuho\u2010ku Nagoya 467\u20108601 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satoru","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Department of Experimental Pathology and Tumor Biology Nagoya City University Graduate School of Medical Sciences 1 Kawasumi Mizuho\u2010cho, Mizuho\u2010ku Nagoya 467\u20108601 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takashi","family":"Joh","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Metabolism Nagoya City University Graduate School of Medical Sciences 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