{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T19:34:08Z","timestamp":1775072048872,"version":"3.50.1"},"reference-count":50,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2014,2,12]],"date-time":"2014-02-12T00:00:00Z","timestamp":1392163200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Hepato Biliary Pancreat"],"published-print":{"date-parts":[[2014,6]]},"abstract":"<jats:title>Abstract\u2003<\/jats:title><jats:p>Cholangiocarcinoma (<jats:styled-content style=\"fixed-case\">CCA<\/jats:styled-content>) is difficult to diagnose at an early stage and most tumors are detected at late stage where surgery or other therapy is ineffective. Many advanced techniques are applied to diagnose <jats:styled-content style=\"fixed-case\">CCA;<\/jats:styled-content> however, most are expensive and have varying degrees of accuracy. A less invasive and simpler procedure such as serum markers would be of substantial clinical benefit for diagnosis, monitoring, and predicting outcome for <jats:styled-content style=\"fixed-case\">CCA<\/jats:styled-content> patients. Recent advances in \u201c<jats:styled-content style=\"fixed-case\">O<\/jats:styled-content>mics\u201d technologies offer remarkable opportunities for establishment of biomarker\u2010related to diseases. In this review, the potential biomarkers obtained from proteomics and glycomic studies are evaluated. Several protein markers were discovered from patient specimen, using two dimensional\u2010differential gel electrophoresis couple with liquid chromatography tandem mass spectrometry (<jats:styled-content style=\"fixed-case\">2D<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">DIGE\/LC\u2010MS\u2010MS<\/jats:styled-content>), matrix\u2010assisted laser desorption\/ionization\u2010time of flight mass spectrometry (<jats:styled-content style=\"fixed-case\">MALDI\u2010TOF\u2010MS<\/jats:styled-content>), surface enhanced laser desorption\/ionization (<jats:styled-content style=\"fixed-case\">SELDI<\/jats:styled-content>)\u2010<jats:styled-content style=\"fixed-case\">TOF\u2010MS<\/jats:styled-content> and capillary electrophoresis (<jats:styled-content style=\"fixed-case\">CE<\/jats:styled-content>)\u2010<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>, etc. Newly reported <jats:styled-content style=\"fixed-case\">CCA<\/jats:styled-content>\u2010associated glyco\u2010biomarkers were identified using lectin\u2010assisted, monoclonal antibody\u2010assisted or specific\u2010target strategies. The combination between carbohydrate binding\u2010lectin and core protein\u2010binding m<jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>b significantly increased the values for detection of the glyco\u2010biomarkers for <jats:styled-content style=\"fixed-case\">CCA<\/jats:styled-content>. Searching for specific and sensitive molecular markers to be used for population screening is worth being evaluated. This could lead to earlier diagnosis and improve outcome. Further investigation of those biomarker functions is also of value in order to better understand the tumor biology and use them as targets for future therapeutic agents.<\/jats:p>","DOI":"10.1002\/jhbp.68","type":"journal-article","created":{"date-parts":[[2014,2,12]],"date-time":"2014-02-12T12:43:15Z","timestamp":1392208995000},"page":"388-396","source":"Crossref","is-referenced-by-count":27,"title":["Cancer biomarker discovery for cholangiocarcinoma: the high\u2010throughput approaches"],"prefix":"10.1002","volume":"21","author":[{"given":"Atit","family":"Silsirivanit","sequence":"first","affiliation":[{"name":"Department of Biochemistry Faculty of Medicine Khon Kaen University  123 Mitraparb Road Khon Kaen 40002 Thailand"},{"name":"Liver Fluke and Cholangiocarcinoma Research Center Faculty of Medicine Khon Kaen University  Khon Kaen 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