{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T10:01:08Z","timestamp":1784887268831,"version":"3.55.0"},"reference-count":26,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T00:00:00Z","timestamp":1774915200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T00:00:00Z","timestamp":1774915200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["24K02254"],"award-info":[{"award-number":["24K02254"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017981","name":"Sysmex Corporation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100017981","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008746","name":"National Cancer Center","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008746","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008746","name":"National Cancer Center","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008746","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Hepato Biliary Pancreat"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>We aimed to establish the tumor cell count and total cell count as surrogate criteria on the basis of the tissue area of biopsy specimen by endoscopic ultrasound\u2010guided tissue acquisition (EUS\u2010TA) for comprehensive genomic profiling (CGP) in pancreatic cancer.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Samples from 114 patients with unresectable pancreatic ductal adenocarcinoma were analyzed. Tumor cells were visually counted, and total cells were quantified with imaging software. Receiver operating characteristic curves determined optimal cutoffs for achieving DNA yield \u2265\u2009200\u2009ng using the OncoGuide NCC Oncopanel.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Thresholds of 2300 tumor cells (AUC 0.653, 95% CI 0.552\u20130.750) and 5923 total cells (AUC 0.671, 95% CI 0.565\u20130.778) were identified. For \u2265\u20092300 tumor cells, sensitivity was 48.7% and specificity 91.2%, with a positive predictive value (PPV) of 92.9%. For \u2265\u20095923 total cells, sensitivity was 52.5% and specificity 82.4%, with a PPV of 87.5%. A clinically practical threshold of 6000 total cells yielded a sensitivity of 48.8%, a specificity of 82.4%, and a PPV of 86.7%.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Tumor cell counts \u2265\u20092300 and total cell counts \u2265\u20096000 may serve as surrogate criteria for tissue area to determine EUS\u2010TA specimen suitability for CGP in pancreatic cancer. Given that tumor cell count is a more biologically relevant indicator, total cell count should be used as a supplementary indicator.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jhbp.70106","type":"journal-article","created":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T04:53:13Z","timestamp":1775019193000},"page":"576-583","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Tumor Cell Count and Total Cell Count as Surrogate Indicators of Comprehensive Genomic Profiling Success in Pancreatic Cancer Specimens Collected by Endoscopic Ultrasonography\u2010Guided Tissue Acquisition"],"prefix":"10.1002","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-7333-7095","authenticated-orcid":false,"given":"Go","family":"Ikeda","sequence":"first","affiliation":[{"name":"Department of Hepatobiliary and Pancreatic Oncology National Cancer Center Hospital  Tokyo Japan"},{"name":"Division of Medical 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Medical University School of Medicine  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takuji","family":"Okusaka","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary and Pancreatic Oncology National Cancer Center Hospital  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nobuyoshi","family":"Hiraoka","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Pathology National Cancer Center Hospital  Tokyo Japan"},{"name":"Division of Innovative Pathology and Laboratory Medicine National Cancer Center EPOC  Tokyo Japan"},{"name":"Division of Molecular Pathology National Cancer Center Research Institute  Tokyo 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