{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T14:35:13Z","timestamp":1788964513838,"version":"build-2803163510"},"reference-count":30,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T00:00:00Z","timestamp":1782950400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T00:00:00Z","timestamp":1782950400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP21K16441"],"award-info":[{"award-number":["JP21K16441"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Hepato Biliary Pancreat"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Cryopreserved venous allografts, which we pioneered in 1999 for use in living donor liver transplantation and hepato\u2010biliary\u2010pancreatic surgery, are widely used and are believed to preserve vascular architecture with minimal anticoagulation or immunosuppression requirements and patency comparable to autologous grafts. However, in contrast to arterial and heart valve grafts, their detailed histopathological characteristics remain unclear.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Histopathological and immunohistochemical analyses were performed in 19 non\u2010transplanted and 15 explanted cryopreserved venous allografts. Cell viability was assessed by culture, and tissue chimerism was evaluated using fluorescence in\u00a0situ hybridization.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The normal trilaminar vascular architecture and presence of viable endothelial cells were confirmed in both groups. No findings of immunologic rejection, including medial cell loss, cellular infiltration in the adventitia, or intimal thickening, were observed. Long\u2010term culture demonstrated sustained viability and proliferative capacity of donor cells. In\u00a0situ hybridization confirmed persistent tissue chimerism with both donor\u2010 and recipient\u2010derived cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Cryopreserved venous allografts maintained viability and showed no histological evidence of active immunologic rejection after long\u2010term implantation. These findings may help explain the favorable clinical performance and provide foundational insights to improve outcomes of venous reconstruction and the development of novel biologic vascular grafts.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jhbp.70148","type":"journal-article","created":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T09:02:18Z","timestamp":1782982938000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Histological Analysis of Cryopreserved Venous Allografts Used for Liver Transplantation and Hepato\u2010Biliary\u2010Pancreatic Surgery"],"prefix":"10.1002","author":[{"given":"Yusuke","family":"Seki","sequence":"first","affiliation":[{"name":"Hepato\u2010Biliary\u2010Pancreatic Surgery Division and Artificial Organ and Transplantation Division, Department of Surgery, Graduate School of Medicine the University of Tokyo  Tokyo 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