{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,4]],"date-time":"2023-09-04T15:48:16Z","timestamp":1693842496189},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[1984,2,1]],"date-time":"1984-02-01T00:00:00Z","timestamp":444441600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1984,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>We have previously demonstrated that peritoneal macrophages (M\u03d5s) from C3H mice were resistant to in vitro infection by ectromelia virus, following activation by intraperitoneal injection of the immunomodulator Corynebacterium parvum. In contrast, resident and mineral oil-elicited M\u03d5s were fully susceptible to virus infection. This report analyzes the infectious cycle of ectromelia virus in C parvum-activated and mineral oil-elicited M\u03d5s and demonstrates that an abortive infection occurred in the activated M\u03d5s that blocked the infectious cycle prior to the release of DNA from the infecting virions. The kinetics of adsorption of radiolabeled virus were similar in both susceptible and resistant M\u03d5 cultures; however, viral-induced incorporation of uridine and thymidine occurred only in the mineral oil-elicited and not the C parvum-activated M\u03d5s. In addition, the late protein hemagglutinin was only detected in infected cultures of susceptible mineral oil-elicited M\u03d5s. An electron micrographic analysis of the infectious cycle indicated that the adsorption of virus to the plasma membrane, uptake into lysosomes, and the primary undercoating and release of viral cores into the M\u03d5 cytoplasm were identical in both M\u03d5 types. In contrast, secondary uncoating (release of genomic DNA from the viral cores into the cytoplasm) was never detected in infected C parvum M\u03d5s. These data are consistent with our previous findings and with the hypothesis that activation of M\u03d5s by C parvum induces an interferon-mediated resistance to ectromelia virus infection.<\/jats:p>","DOI":"10.1002\/jlb.35.2.179","type":"journal-article","created":{"date-parts":[[2018,1,3]],"date-time":"2018-01-03T09:30:07Z","timestamp":1514971807000},"page":"179-192","source":"Crossref","is-referenced-by-count":7,"title":["Abortive Ectromelia Virus Infection in Peritoneal Macrophages Activated by Corynebacterium parvum"],"prefix":"10.1093","volume":"35","author":[{"given":"Donald A","family":"Cohen","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology and Immunology, University of Kentucky, College of Medicine , Lexington"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Randal E","family":"Morris","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, University of Cincinnati, College of Medicine , Cincinnati"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H Curt","family":"Bubel","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, University of Cincinnati, College of Medicine , Cincinnati"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[1984,2,1]]},"container-title":["Journal of Leukocyte Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjlb.35.2.179","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/jleukbio\/article-pdf\/35\/2\/179\/49724295\/jlb0179.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jleukbio\/article-pdf\/35\/2\/179\/49724295\/jlb0179.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,3]],"date-time":"2023-04-03T13:24:55Z","timestamp":1680528295000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jleukbio\/article\/35\/2\/179\/6977844"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1984,2,1]]},"references-count":0,"journal-issue":{"issue":"2","published-online":{"date-parts":[[1984,2,1]]},"published-print":{"date-parts":[[1984,2,1]]}},"URL":"https:\/\/doi.org\/10.1002\/jlb.35.2.179","relation":{},"ISSN":["0741-5400","1938-3673"],"issn-type":[{"value":"0741-5400","type":"print"},{"value":"1938-3673","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[1984,2]]},"published":{"date-parts":[[1984,2,1]]}}}