{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T11:28:06Z","timestamp":1779881286242,"version":"3.53.1"},"reference-count":36,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2018,9,10]],"date-time":"2018-09-10T00:00:00Z","timestamp":1536537600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"},{"start":{"date-parts":[[2018,9,10]],"date-time":"2018-09-10T00:00:00Z","timestamp":1536537600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2018,9,10]],"date-time":"2018-09-10T00:00:00Z","timestamp":1536537600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2018,12,27]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Glucocorticoids (GCs) are potent anti-allergic compounds that function, at least in part, by inhibiting signaling pathways in mast cells. We hypothesized that the GC-induced mastocytopenia and suppression of mast cell activation are mediated by the advanced glycation end products (AGEs)\/receptors of AGEs (RAGEs) signaling axis. We evaluated the role of AGEs in GC-mediated mastocytopenia and impaired mast cell degranulation in male Wistar rats and Swiss\u2013Webster mice subcutaneously injected with dexamethasone or prednisolone (0.1 mg\/kg) once a day for 21 consecutive days. The animals were treated with either the AGE inhibitor aminoguanidine (250 mg\/kg), the RAGE antagonist FPS-ZM1 (1 mg\/kg) or the galectin-3 antagonist GSC-100 (1 mg\/kg) daily for 18 days, starting 3 days following GC treatment. Aminoguanidine inhibited GC-induced mast cell apoptosis and restored mast cell numbers in the pleural cavity of GC-treated rats. Aminoguanidine also reversed the GC-induced reduction in histamine release triggered by allergens or compound 48\/80 in vitro. GC treatment induced RAGE and galectin expression in mast cells, and blocking these agents by FPS-ZM1 or GSC-100 significantly reversed mast cell numbers in the peritoneal cavity and mesenteric tissue of GC-treated mice. In addition, the combination of GC and AGE-induced mast cell apoptosis in vitro was inhibited by both FPS-ZM1 and GSC-100. We concluded that the GC-induced mastocytopenia and suppression of mast cell stimulation are associated with the gene transactivation of RAGE and galectin-3.<\/jats:p><jats:p>Glucocorticoids decrease the number and activation of mast cells by upregulating receptors of AGEs, a mechanism dissociated from their immunosuppressive and metabolic effects.<\/jats:p>","DOI":"10.1002\/jlb.3a0917-364rr","type":"journal-article","created":{"date-parts":[[2018,9,10]],"date-time":"2018-09-10T14:05:57Z","timestamp":1536588357000},"page":"131-142","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Glucocorticoids decrease the numbers and activation of mast cells by inducing the transactivation receptors of AGEs"],"prefix":"10.1093","volume":"105","author":[{"given":"Tassia","family":"Santoro","sequence":"first","affiliation":[{"name":"Laboratory of Inflammation, Oswaldo Cruz Institute, Oswaldo Cruz Foundation , Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina T","family":"Azevedo","sequence":"additional","affiliation":[{"name":"Laboratory of Inflammation, Oswaldo Cruz Institute, Oswaldo Cruz Foundation , Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patr\u00edcia M R","family":"e Silva","sequence":"additional","affiliation":[{"name":"Laboratory of Inflammation, Oswaldo Cruz Institute, Oswaldo Cruz Foundation , Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco A","family":"Martins","sequence":"additional","affiliation":[{"name":"Laboratory of Inflammation, Oswaldo Cruz Institute, Oswaldo Cruz Foundation , Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vinicius F","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Laboratory of Inflammation, Oswaldo Cruz Institute, Oswaldo Cruz Foundation , Rio de Janeiro, Brazil"},{"name":"National Institute of Science and Technology on Neuroimmunomodulation (INCT-NIM) , Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2018,9,10]]},"reference":[{"key":"2023032406272719700_jlb10238-cit-0001","doi-asserted-by":"crossref","first-page":"1299","DOI":"10.1189\/jlb.2HI0317-088RR","article-title":"Frontline Science: corticotropin-releasing factor receptor subtype 1 is a critical modulator of mast cell degranulation and stress-induced pathophysiology","volume":"102","author":"Ayyadurai","year":"2017","journal-title":"J Leukoc Biol"},{"key":"2023032406272719700_jlb10238-cit-0002","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1111\/imr.12557","article-title":"Innate and adaptive type 2 immunity in lung allergic inflammation","volume":"278","author":"Kubo","year":"2017","journal-title":"Immunol 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