{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T13:58:32Z","timestamp":1762955912024,"version":"3.37.3"},"reference-count":53,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2018,5,7]],"date-time":"2018-05-07T00:00:00Z","timestamp":1525651200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100001711","name":"Swiss National Science Foundation","doi-asserted-by":"publisher","award":["#31003A_173215","#310030_166473","R21AI116121"],"award-info":[{"award-number":["#31003A_173215","#310030_166473","R21AI116121"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]},{"name":"C.H.F.","award":["T32ES007059"],"award-info":[{"award-number":["T32ES007059"]}]},{"name":"Microscopy Imaging Centre of the University of Bern"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,6,21]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The asthmatic airways are highly susceptible to inflammatory injury by air pollutants such as ozone (O3), characterized by enhanced activation of eosinophilic granulocytes and a failure of immune protective mechanisms. Eosinophil activation during asthma exacerbation contributes to the proinflammatory oxidative stress by high levels of nitric oxide (NO) production and extracellular DNA release. Surfactant protein-D (SP-D), an epithelial cell product of the airways, is a critical immune regulatory molecule with a multimeric structure susceptible to oxidative modifications. Using recombinant proteins and confocal imaging, we demonstrate here that SP-D directly bound to the membrane and inhibited extracellular DNA trap formation by human and murine eosinophils in a concentration and carbohydrate-dependent manner. Combined allergic airway sensitization and O3 exposure heightened eosinophilia and nos2 mRNA (iNOS) activation in the lung tissue and S-nitrosylation related de-oligomerisation of SP-D in the airways. In vitro reproduction of the iNOS action led to similar effects on SP-D. Importantly, S-nitrosylation abolished the ability of SP-D to block extracellular DNA trap formation. Thus, the homeostatic negative regulatory feedback between SP-D and eosinophils is destroyed by the NO-rich oxidative lung tissue environment in asthma exacerbations.<\/jats:p>\n               <jats:p>Demonstration that specific carbohydrate-dependent inhibition of murine and human eosinophil extracellular DNA trap release is abolished by s-nitrosylation of the SP-D molecule.<\/jats:p>","DOI":"10.1002\/jlb.3ab1117-455r","type":"journal-article","created":{"date-parts":[[2018,5,7]],"date-time":"2018-05-07T16:00:12Z","timestamp":1525708812000},"page":"205-214","source":"Crossref","is-referenced-by-count":35,"title":["Oxidative damage of SP-D abolishes control of eosinophil extracellular DNA trap formation"],"prefix":"10.1093","volume":"104","author":[{"given":"Shida","family":"Yousefi","sequence":"first","affiliation":[{"name":"University of Bern , Bern, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satish 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H","family":"Flayer","sequence":"additional","affiliation":[{"name":"University of California , Davis, California, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erik D","family":"Larson","sequence":"additional","affiliation":[{"name":"University of California , Davis, California, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Imre G","family":"Redai","sequence":"additional","affiliation":[{"name":"University of Pennsylvania , Philadelphia, Pennsylvania, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suhong","family":"Zhang","sequence":"additional","affiliation":[{"name":"University of Pennsylvania , Philadelphia, Pennsylvania, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cynthia J","family":"Koziol-White","sequence":"additional","affiliation":[{"name":"University of Pennsylvania , Philadelphia, Pennsylvania, USA"},{"name":"Rutgers University , New Brunswick, New Jersey, 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