{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T19:46:31Z","timestamp":1792525591753,"version":"4.5.0"},"reference-count":41,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2022,2,9]],"date-time":"2022-02-09T00:00:00Z","timestamp":1644364800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"},{"start":{"date-parts":[[2022,2,9]],"date-time":"2022-02-09T00:00:00Z","timestamp":1644364800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2022,2,9]],"date-time":"2022-02-09T00:00:00Z","timestamp":1644364800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2022,4,21]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Intestinal eosinophils are implicated in the inflammatory pathology of eosinophilic gastrointestinal diseases and inflammatory bowel diseases. Eosinophils also contribute to intestinal immunologic and tissue homeostasis and host defense. Recent studies in allergic airway disease suggest functional subphenotypes of eosinophils may underly their pathogenic versus protective roles. However, subphenotypes of intestinal eosinophils have not been defined and are complicated by their constitutive expression of the putative eosinophil inflammatory marker CD11c. Here, we propose a framework for subphenotype characterization of intestinal eosinophils based on relative intensity of surface CD11c expression. Using this flow cytometry framework in parallel with histology and BrdU tracing, we characterize intestinal eosinophil subphenotypes and monitor their plasticity at baseline and within the context of acute allergic and chronic systemic inflammation. Data reveal a conserved continuum of CD11c expression amongst intestinal eosinophils in health and acute disease states that overall tracked with other markers of activation. Oral allergen challenge induced recruitment of eosinophils into small intestinal lamina propria surrounding crypts, followed by in situ induction of CD11c expression in parallel with eosinophil redistribution into intestinal villi. Allergen challenge also elicited eosinophil transepithelial migration and the appearance of CD11cloCD11bhi eosinophils in the intestinal lumen. Chronic inflammation driven by overexpression of TNF\u03b1 led to a qualitative shift in the relative abundance of CD11c-defined eosinophil subphenotypes favoring CD11chi-expressing eosinophils. These findings provide new insights into heterogeneity of intestinal tissue eosinophils and offer a framework for measuring and tracking eosinophil subphenotype versatility in situ in health and disease.<\/jats:p>","DOI":"10.1002\/jlb.3hi0821-432rr","type":"journal-article","created":{"date-parts":[[2022,2,10]],"date-time":"2022-02-10T06:18:21Z","timestamp":1644473901000},"page":"943-952","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Modulation of surface CD11c expression tracks plasticity in murine intestinal tissue eosinophils"],"prefix":"10.1093","volume":"111","author":[{"given":"Leigha D","family":"Larsen","sequence":"first","affiliation":[{"name":"Gastrointestinal Eosinophilic Diseases Program, Digestive Health Institute, Children's Hospital Colorado, University of Colorado School of Medicine , Aurora, Colorado, USA"},{"name":"Department of Pediatrics, University of Colorado School of Medicine , Aurora, Colorado, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen","family":"Dockstader","sequence":"additional","affiliation":[{"name":"Gastrointestinal Eosinophilic Diseases Program, Digestive Health Institute, Children's Hospital Colorado, University of Colorado School of Medicine , Aurora, Colorado, USA"},{"name":"Department of Pediatrics, University of Colorado School of Medicine , Aurora, Colorado, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Courtney L","family":"Olbrich","sequence":"additional","affiliation":[{"name":"Gastrointestinal Eosinophilic Diseases Program, Digestive Health Institute, Children's Hospital Colorado, University of Colorado School of Medicine , Aurora, Colorado, USA"},{"name":"Department of Pediatrics, University of Colorado School of Medicine , Aurora, Colorado, USA"},{"name":"GI and Liver Innate Immune Program (GALIIP), and Mucosal Inflammation Program (MIP), University of Colorado School of Medicine , Aurora, Colorado, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ian M","family":"Cartwright","sequence":"additional","affiliation":[{"name":"GI and Liver Innate Immune Program (GALIIP), and Mucosal Inflammation Program (MIP), University of Colorado School of Medicine , Aurora, Colorado, USA"},{"name":"Department of Medicine, University of Colorado School of Medicine , Aurora, Colorado, USA"},{"name":"Rocky Mountain Regional Veterans Affairs Medical Center , Aurora, Colorado, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa A","family":"Spencer","sequence":"additional","affiliation":[{"name":"Gastrointestinal Eosinophilic Diseases Program, Digestive Health Institute, Children's Hospital Colorado, University of Colorado School of Medicine , Aurora, Colorado, USA"},{"name":"Department of Pediatrics, University of Colorado School of Medicine , Aurora, Colorado, USA"},{"name":"GI and Liver Innate Immune Program (GALIIP), and Mucosal Inflammation Program (MIP), University of Colorado School of Medicine , Aurora, Colorado, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,2,9]]},"reference":[{"key":"2023032109025422400_","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1146\/annurev-pathmechdis-012419-032756","article-title":"Contributions of eosinophils to human health and disease","volume":"15","author":"Klion","year":"2020","journal-title":"Annu Rev Pathol"},{"key":"2023032109025422400_","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1615\/CritRevImmunol.2016018726","article-title":"Eosinophils in homeostasis and their contrasting roles during inflammation and helminth infections","volume":"36","author":"Strandmark","year":"2016","journal-title":"Crit Rev Immunol"},{"key":"2023032109025422400_","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1159\/000504847","article-title":"The cellular functions of eosinophils: Collegium 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