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In general, its chemotaxis and activation are benefit for the host defense of bacterial infection and inflammation. However, previous studies have reported that the hyperactive and reactive neutrophils appear to be one of the reasons for tissue destruction in periodontitis tissues. In this study, we investigated an isoquinoline alkaloid Litcubanine A (LA), which from the Traditional Chinese medicinal plant, Litsea cubeba. We found LA showed significant activity in inhibiting neutrophils chemotaxis in the zebrafish yolk sac microinjection model in vivo and in mouse neutrophils in vitro. Further investigation proved that LA could inhibit the expression levels of neutrophil respiratory burst-related and inflammation-related genes CYBB and NCF2, as well as inhibit the activation of MAPK signaling pathway. Moreover, using LA, we successfully achieved the effect of reducing periodontitis bone loss by regulating neutrophil chemotaxis and related functions in a mouse ligature-induced periodontitis model.<\/jats:p>","DOI":"10.1002\/jlb.3ma0321-736r","type":"journal-article","created":{"date-parts":[[2021,6,29]],"date-time":"2021-06-29T06:46:01Z","timestamp":1624949161000},"page":"475-484","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["The effect of an isoquinoline alkaloid on treatment of periodontitis by regulating the neutrophils chemotaxis"],"prefix":"10.1093","volume":"110","author":[{"given":"Yitong","family":"Liu","sequence":"first","affiliation":[{"name":"Laboratory of Tissue Regeneration and Immunology and Department of Periodontics, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, School of Stomatology, Capital Medical University , Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huan","family":"Xia","sequence":"additional","affiliation":[{"name":"Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine , Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guiyang","family":"Xia","sequence":"additional","affiliation":[{"name":"Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine , Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheng","family":"Lin","sequence":"additional","affiliation":[{"name":"Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine , Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijia","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Orthodontics School of Stomatology, Capital Medical University , Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Liu","sequence":"additional","affiliation":[{"name":"Laboratory of Tissue Regeneration and Immunology and Department of Periodontics, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, School of Stomatology, Capital Medical University , Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2021,6,28]]},"reference":[{"key":"2023032108463877300_","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.it.2013.09.001","article-title":"Immunomicrobial pathogenesis of periodontitis: keystones, pathobionts, and host 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