{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T12:01:29Z","timestamp":1792670489273,"version":"4.5.0"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[1991,6,1]],"date-time":"1991-06-01T00:00:00Z","timestamp":675734400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1991,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>THP-1 cells are a monocyte-like cell line derived from a patient with acute monocytic leukemia and unlike other leukemic cell lines has a normal diploid karyotype. We have characterized Fc\u03b3R expression on this cell line by flow cytometry, radiolabeled lgG1 and monoclonal antibody (mAb) binding assays, and biochemical analysis. Flow cytometric analysis of THP-1 cells with anti-Fc\u03b3RI, II, and III mAb, and a rabbit anti-Fc\u03b3RIII F(ab')2 demonstrated that only Fc\u03b3RI and FC\u03b3RII are expressed by these cells. A panel of anti-Fc\u03b3RIII mAb (anti-CD16) failed to bind to THP-1 cells. Biochemical studies identified polypeptides of 64 to 78 kDa (Fc\u03b3RI) and of 42 to 53 kDa (Fc\u03b3RII). Fc\u03b3R expression was determined by binding of radioiodinated human lgG1 (to detect Fc\u03b3RI), mAb IV.3 (to detect Fc\u03b3RII), or rabbit IgG immune complexes. Thirty-five thousand high affinity binding sites (dissociation constant [KD] = 4.22 \u00d7 10-9M) for lgG1 were found on THP-1 cells. Interferon-\u03b3 (IFN\u03b3) upregulated Fc\u03b3RI expression by THP-1 cells 2.8-fold, whereas Fc\u03b3RI on U937 cells was increased six- to eight-fold by this cytokine. Phorbol myristate acetate (PMA), tumor necrosis factor-\u03b1 (TNF\u03b1), and vitamin D3 had no effect on lgG1 binding by THP-1 cells. Fifty thousand IgG molecules in immune complexes bound to THP-1 cells. IFN\u03b3 treatment increased this binding by four-fold, PMA treatment resulted in a 50% increase in the number of IgG immune complexes bound, whereas vitamin D3 treated THP-1 cells bound half as many IgG immune complexes as control cells. Binding assays utilizing mAb IV.3 identified 50,000 sites per cell. Treatment of THP-1 cells with IFN\u03b3, TNF\u03b1, PMA, or vitamin D3 had no effect on Fc\u03b3RII expression. That Fc\u03b3RI plays a predominant role in immune complex binding was demonstrated by inhibition studies. Human lgG1 as well as mouse lgG2a mAb to Fc\u03b3RII inhibited immune complex binding by 76 to 84%, whereas mouse lgG1 mAb to Fc\u03b3RII had minimal effect on immune complex binding. Fc\u03b3R expression may not be linked to differentiation of THP-1 cells since only 1,25 vitamin D3 was able to induce the expression of CD14, a marker of mature monocytic phenotype.<\/jats:p>","DOI":"10.1002\/jlb.49.6.556","type":"journal-article","created":{"date-parts":[[2018,1,12]],"date-time":"2018-01-12T19:48:49Z","timestamp":1515786529000},"page":"556-565","source":"Crossref","is-referenced-by-count":122,"title":["The Human Monocyte-Like Cell Line THP-1 Expresses Fc\u03b3RI and FC\u03b3RII"],"prefix":"10.1093","volume":"49","author":[{"given":"Howard B","family":"Fleit","sequence":"first","affiliation":[{"name":"Department of Pathology, State University of New York at Stony Brook , Stony Brook"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine D","family":"Kobasiuk","sequence":"additional","affiliation":[{"name":"Department of Pathology, State University of New York at Stony Brook , Stony Brook"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[1991,6,1]]},"container-title":["Journal of Leukocyte Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjlb.49.6.556","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/jleukbio\/article-pdf\/49\/6\/556\/49767214\/jlb0556.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jleukbio\/article-pdf\/49\/6\/556\/49767214\/jlb0556.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,11]],"date-time":"2023-04-11T08:36:49Z","timestamp":1681202209000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jleukbio\/article\/49\/6\/556\/6977603"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,6,1]]},"references-count":0,"journal-issue":{"issue":"6","published-online":{"date-parts":[[1991,6,1]]},"published-print":{"date-parts":[[1991,6,1]]}},"URL":"https:\/\/doi.org\/10.1002\/jlb.49.6.556","relation":{},"ISSN":["0741-5400","1938-3673"],"issn-type":[{"value":"0741-5400","type":"print"},{"value":"1938-3673","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[1991,6]]},"published":{"date-parts":[[1991,6,1]]}}}