{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T21:59:21Z","timestamp":1788904761221,"version":"build-2803163510"},"reference-count":64,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2020,7,14]],"date-time":"2020-07-14T00:00:00Z","timestamp":1594684800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"},{"start":{"date-parts":[[2020,7,14]],"date-time":"2020-07-14T00:00:00Z","timestamp":1594684800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2020,7,14]],"date-time":"2020-07-14T00:00:00Z","timestamp":1594684800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Rio de Janeiro"},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"name":"US National Institute of Health","award":["NHLBI 5R37HLO44525"],"award-info":[{"award-number":["NHLBI 5R37HLO44525"]}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2020,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Dengue is characterized as one of the most important arthropod-borne human viral diseases, representing a public health problem. Increased activation of immune cells is involved in the progression of infection to severe forms. Recently, our group demonstrated the contribution of platelet\u2013monocyte interaction to inflammatory responses in dengue, adding to evolving evidence that platelets have inflammatory functions and can regulate different aspects of innate immune responses. Furthermore, stimuli-specific-activated platelets can promote phenotypic changes and metabolic reprogramming in monocytes. Thus, this study aimed to evaluate the roles of dengue virus (DENV)-activated platelets on immunometabolic reprogramming of monocytes in vitro, focusing on lipid droplet (LD) biogenesis. We demonstrated that platelets exposed to DENV in vitro form aggregates with monocytes and signal to LD formation and CXCL8\/IL-8, IL-10, CCL2, and PGE2 secretion. Pharmacologic inhibition of LD biogenesis prevents PGE2 secretion, but not CXCL8\/IL-8 release, by platelet\u2013monocyte complexes. In exploring the mechanisms involved, we demonstrated that LD formation in monocytes exposed to DENV-activated platelets is partially dependent on platelet-produced MIF. Additionally, LD formation is higher in monocytes, which have platelets adhered on their surface, suggesting that beyond paracrine signaling, platelet adhesion is an important event in platelet-mediated modulation of lipid metabolism in monocytes. Together, our results demonstrate that activated platelets aggregate with monocytes during DENV infection and signal to LD biogenesis and the secretion of inflammatory mediators, which may contribute to dengue immunopathogenesis.<\/jats:p>","DOI":"10.1002\/jlb.4ma0620-658r","type":"journal-article","created":{"date-parts":[[2020,7,14]],"date-time":"2020-07-14T21:27:06Z","timestamp":1594762026000},"page":"1293-1306","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Dengue virus-activated platelets modulate monocyte immunometabolic response through lipid droplet biogenesis and cytokine signaling"],"prefix":"10.1093","volume":"108","author":[{"given":"Giselle","family":"Barbosa-Lima","sequence":"first","affiliation":[{"name":"Laboratory of Immunopharmacology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation , Rio de Janeiro, RJ , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2201-1742","authenticated-orcid":true,"given":"Eugenio D","family":"Hottz","sequence":"additional","affiliation":[{"name":"Laboratory of Immunopharmacology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation , Rio de Janeiro, RJ , Brazil"},{"name":"Laboratory of Immunothrombosis, Department of Biochemistry, Federal University of Juiz de Fora (UFJF) , Juiz de Fora, MG , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edson F","family":"de Assis","sequence":"additional","affiliation":[{"name":"Laboratory of Immunopharmacology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation , Rio de Janeiro, RJ , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sally","family":"Liechocki","sequence":"additional","affiliation":[{"name":"Laboratory of Immunopharmacology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation , Rio de Janeiro, RJ , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thiago Moreno L","family":"Souza","sequence":"additional","affiliation":[{"name":"Laboratory of Immunopharmacology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation , Rio de Janeiro, RJ , Brazil"},{"name":"National Institute for Science and Technology on Innovation on Neglected Diseases (INCT\/IDN), Center for Technological Development in Health (CDTS), Oswaldo Cruz Foundation , Rio de Janeiro, RJ , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guy A","family":"Zimmerman","sequence":"additional","affiliation":[{"name":"Molecular Medicine Program and Department of Internal Medicine, University of Utah , Salt Lake City, Utah , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8349-9529","authenticated-orcid":true,"given":"Fernando A","family":"Bozza","sequence":"additional","affiliation":[{"name":"Laboratory of Immunopharmacology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation , Rio de Janeiro, RJ , Brazil"},{"name":"Intensive Medicine Laboratory, National Institute of Infectious Disease Evandro Chagas, Oswaldo Cruz Foundation , Rio de Janeiro, RJ , Brazil"},{"name":"D\u2019Or Institute of Research , Rio de Janeiro, RJ , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia T","family":"Bozza","sequence":"additional","affiliation":[{"name":"Laboratory of Immunopharmacology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation , Rio de Janeiro, RJ , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,7,14]]},"reference":[{"key":"2023032108070696000_jlb10758-cit-0001","doi-asserted-by":"crossref","first-page":"16055","DOI":"10.1038\/nrdp.2016.55","article-title":"Dengue infection","volume":"2","author":"Guzman","year":"2016","journal-title":"Nat Rev Dis Prim"},{"key":"2023032108070696000_jlb10758-cit-0002","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1016\/S0140-6736(18)32560-1","article-title":"Dengue","volume":"393","author":"Wilder-Smith","year":"2019","journal-title":"Lancet"},{"key":"2023032108070696000_jlb10758-cit-0003","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1038\/nature12060","article-title":"The global distribution and burden of dengue","volume":"496","author":"Bhatt","year":"2013","journal-title":"Nature"},{"key":"2023032108070696000_jlb10758-cit-0004","first-page":"147","article-title":"Dengue: guidelines for diagnosis, treatment, prevention, and control","volume":"x","author":"World Health Organization","year":"2009","journal-title":"Spec Program Res Train Trop Dis"},{"key":"2023032108070696000_jlb10758-cit-0005","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1186\/1471-2334-8-86","article-title":"Multiplex cytokine profile from dengue patients: mIP-1beta and IFN-gamma as predictive factors for severity","volume":"8","author":"A","year":"2008","journal-title":"BMC Infect Dis"},{"key":"2023032108070696000_jlb10758-cit-0006","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1038\/sj.icb.7100008","article-title":"Of cascades and perfect storms: the immunopathogenesis of dengue haemorrhagic fever-dengue shock syndrome (DHF\/DSS)","volume":"85","author":"Pang","year":"2007","journal-title":"Immunol Cell Biol"},{"key":"2023032108070696000_jlb10758-cit-0007","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1007\/s00281-017-0625-1","article-title":"Immune-mediated cytokine storm and its role in severe dengue","volume":"39","author":"Srikiatkhachorn","year":"2017","journal-title":"Semin Immunopathol"},{"key":"2023032108070696000_jlb10758-cit-0008","doi-asserted-by":"crossref","first-page":"121","DOI":"10.3389\/fmed.2018.00121","article-title":"Platelets in immune response to virus and immunopathology of viral infections","volume":"5","author":"Hottz","year":"2018","journal-title":"Front Med"},{"key":"2023032108070696000_jlb10758-cit-0009","first-page":"145","article-title":"Thrombocytopenia in dengue fever","volume":"4","author":"Schexneider","year":"2005","journal-title":"Curr Hematol Rep"},{"key":"2023032108070696000_jlb10758-cit-0010","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1080\/09537100701426604","article-title":"Thrombocytopenia in patients with dengue virus infection in the Brazilian Amazon","volume":"18","author":"Mour\u00e3o","year":"2007","journal-title":"Platelets"},{"key":"2023032108070696000_jlb10758-cit-0011","doi-asserted-by":"crossref","first-page":"e33","DOI":"10.1016\/j.ddmec.2011.09.001","article-title":"Platelets in dengue infection","volume":"8","author":"Hottz","year":"2011","journal-title":"Drug Discov Today Dis Mech"},{"key":"2023032108070696000_jlb10758-cit-0012","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1161\/01.CIR.0000154607.90506.45","article-title":"Dipyridamole selectively inhibits inflammatory gene expression in platelet-monocyte aggregates","volume":"111","author":"Weyrich","year":"2005","journal-title":"Circulation"},{"key":"2023032108070696000_jlb10758-cit-0013","doi-asserted-by":"crossref","first-page":"1525","DOI":"10.1172\/JCI118575","article-title":"Activated platelets signal chemokine synthesis by human monocytes","volume":"97","author":"Weyrich","year":"1996","journal-title":"J Clin Invest"},{"key":"2023032108070696000_jlb10758-cit-0014","doi-asserted-by":"crossref","first-page":"1897","DOI":"10.1046\/j.1538-7836.2003.00304.x","article-title":"The evolving role of platelets in inflammation","volume":"1","author":"Weyrich","year":"2003","journal-title":"J Thromb Haemost"},{"key":"2023032108070696000_jlb10758-cit-0015","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1007\/s00281-011-0286-4","article-title":"Platelets: versatile effector cells in hemostasis, inflammation, and the immune continuum","volume":"34","author":"Vieira-de-Abreu","year":"2012","journal-title":"Semin Immunopathol"},{"key":"2023032108070696000_jlb10758-cit-0016","doi-asserted-by":"crossref","first-page":"2727","DOI":"10.1172\/JCI27209.","article-title":"Expression of COX-2 in platelet-monocyte interactions occurs via combinatorial regulation involving adhesion and cytokine signaling","volume":"116","author":"Dixon","year":"2006","journal-title":"J Clin Invest"},{"key":"2023032108070696000_jlb10758-cit-0017","doi-asserted-by":"crossref","first-page":"10284","DOI":"10.1073\/pnas.181201398","article-title":"Cell adhesion regulates gene expression at translational checkpoints in human myeloid leukocytes","volume":"98","author":"Mahoney","year":"2001","journal-title":"Proc Natl Acad Sci USA"},{"key":"2023032108070696000_jlb10758-cit-0018","doi-asserted-by":"crossref","first-page":"3623","DOI":"10.1099\/vir.0.82093-0","article-title":"MCP-1, a highly expressed chemokine in dengue haemorrhagic fever\/dengue shock syndrome patients, may cause permeability change, possibly through reduced tight junctions of vascular endothelium cells","volume":"87","author":"Lee","year":"2006","journal-title":"J Gen Virol"},{"issue":"Suppl 1","key":"2023032108070696000_jlb10758-cit-0019","first-page":"S16","article-title":"The role of prostacyclin (PGI2) and thromboxane A2 (TXA2) in pathogenesis of dengue hemorrhagic fever (DHF)","volume":"82","author":"Preeyasombat","year":"1999","journal-title":"J Med Assoc Thai"},{"key":"2023032108070696000_jlb10758-cit-0020","doi-asserted-by":"crossref","first-page":"44701","DOI":"10.1038\/srep44701","article-title":"Cyclooxygenase-2 facilitates dengue virus replication and serves as a potential target for developing antiviral agents","volume":"7","author":"Lin","year":"2017","journal-title":"Sci Rep"},{"key":"2023032108070696000_jlb10758-cit-0021","doi-asserted-by":"crossref","first-page":"3413","DOI":"10.1002\/eji.200939306","article-title":"Triggering of DC migration by dengue virus stimulation of COX-2-dependent signaling cascades in vitro highlights the significance of these cascades beyond inflammation","volume":"39","author":"Wu","year":"2009","journal-title":"Eur J Immunol"},{"key":"2023032108070696000_jlb10758-cit-0022","doi-asserted-by":"crossref","first-page":"896","DOI":"10.1016\/j.celrep.2019.06.062","article-title":"Platelets promote macrophage polarization toward pro-inflammatory phenotype and increase survival of septic mice","volume":"28","author":"Carestia","year":"2019","journal-title":"Cell Rep"},{"key":"2023032108070696000_jlb10758-cit-0023","doi-asserted-by":"crossref","first-page":"1700","DOI":"10.1093\/infdis\/jiu355","article-title":"Platelets direct monocyte differentiation into epithelioid-like multinucleated giant foam cells with suppressive capacity upon mycobacterial stimulation","volume":"210","author":"Feng","year":"2014","journal-title":"J Infect Dis"},{"key":"2023032108070696000_jlb10758-cit-0024","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.tube.2018.05.002","article-title":"Platelet-monocyte interaction in Mycobacterium tuberculosis infection","volume":"111","author":"Kullaya","year":"2018","journal-title":"Tuberculosis"},{"key":"2023032108070696000_jlb10758-cit-0025","doi-asserted-by":"crossref","first-page":"951","DOI":"10.1111\/jth.12178","article-title":"Dengue induces platelet activation, mitochondrial dysfunction and cell death through mechanisms that involve DC-SIGN and caspases","volume":"11","author":"Hottz","year":"2013","journal-title":"J Thromb Haemost"},{"key":"2023032108070696000_jlb10758-cit-0026","doi-asserted-by":"crossref","first-page":"1864","DOI":"10.4049\/jimmunol.1400091","article-title":"Platelet activation and apoptosis modulate monocyte inflammatory responses in dengue","volume":"193","author":"Hottz","year":"2014","journal-title":"J Immunol"},{"key":"2023032108070696000_jlb10758-cit-0027","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1161\/ATVBAHA.113.302919","article-title":"Platelets mediate oxidized low-density lipoprotein\u2013 induced monocyte extravasation and foam cell formation","volume":"34","author":"Badrnya","year":"2014","journal-title":"Arterioscler Thromb Vasc Biol"},{"key":"2023032108070696000_jlb10758-cit-0028","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1016\/j.bbalip.2009.01.005","article-title":"Leukocyte lipid bodies - Biogenesis and functions in inflammation","volume":"1791","author":"Bozza","year":"2009","journal-title":"Biochim Biophys Acta Mol Cell Biol Lipids"},{"key":"2023032108070696000_jlb10758-cit-0029","doi-asserted-by":"crossref","first-page":"909","DOI":"10.1084\/jem.186.6.909","article-title":"Eosinophil lipid bodies: specific, inducible intracellular sites for enhanced eicosanoid formation","volume":"186","author":"Bozza","year":"1997","journal-title":"J Exp Med"},{"key":"2023032108070696000_jlb10758-cit-0030","doi-asserted-by":"crossref","first-page":"8500","DOI":"10.4049\/jimmunol.179.12.8500","article-title":"Monocyte chemoattractant protein-1\/CC chemokine ligand 2 controls microtubule-driven biogenesis and leukotriene B4-synthesizing function of macrophage lipid bodies elicited by innate immune response","volume":"179","author":"Pacheco","year":"2007","journal-title":"J Immunol"},{"key":"2023032108070696000_jlb10758-cit-0031","doi-asserted-by":"crossref","first-page":"22779","DOI":"10.1074\/jbc.M101436200","article-title":"Extranuclear lipid bodies, elicited by CCR3-mediated signaling pathways, are the sites of chemokine-enhanced leukotriene C4 production in eosinophils and basophils","volume":"276","author":"Bandeira-Melo","year":"2001","journal-title":"J Biol Chem"},{"key":"2023032108070696000_jlb10758-cit-0032","doi-asserted-by":"crossref","first-page":"1308","DOI":"10.1016\/j.intimp.2008.01.035","article-title":"Lipid bodies in innate immune response to bacterial and parasite infections","volume":"8","author":"D\u2019Avila","year":"2008","journal-title":"Int Immunopharmacol"},{"key":"2023032108070696000_jlb10758-cit-0033","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1096\/fj.09-139469","article-title":"Contribution of macrophage migration inhibitory factor to the pathogenesis of dengue virus infection","volume":"24","author":"Assun\u00e7\u00e3o-Miranda","year":"2010","journal-title":"FASEB J"},{"key":"2023032108070696000_jlb10758-cit-0034","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1000632","article-title":"Dengue virus capsid protein usurps lipid droplets for viral particle formation","author":"Samsa","year":"2009","journal-title":"PLoS Pathog"},{"key":"2023032108070696000_jlb10758-cit-0035","doi-asserted-by":"crossref","first-page":"1732","DOI":"10.1158\/0008-5472.CAN-07-1999","article-title":"Lipid bodies are reservoirs of cyclooxygenase-2 and sites of prostaglandin-E2 synthesis in colon cancer cells","volume":"68","author":"Accioly","year":"2008","journal-title":"Cancer Res"},{"key":"2023032108070696000_jlb10758-cit-0036","doi-asserted-by":"crossref","first-page":"e873","DOI":"10.1371\/journal.pntd.0000873","article-title":"Lutzomyia longipalpis saliva triggers lipid body formation and prostaglandin E2 production in murine macrophages","volume":"4","author":"Ara\u00fajo-Santos","year":"2010","journal-title":"PLoS Negl Trop Dis"},{"key":"2023032108070696000_jlb10758-cit-0037","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1165\/rcmb.2005-0145OC","article-title":"Allergic challenge-elicited lipid bodies compartmentalize in vivo leukotriene C4 synthesis within eosinophils","volume":"33","author":"Vieira-De-Abreu","year":"2005","journal-title":"Am J Respir Cell Mol Biol"},{"key":"2023032108070696000_jlb10758-cit-0038","doi-asserted-by":"crossref","first-page":"3087","DOI":"10.4049\/jimmunol.176.5.3087","article-title":"Mycobacterium bovis bacillus Calmette-Gu\u00e9rin induces TLR2-mediated formation of lipid bodies: intracellular domains for eicosanoid synthesis in vivo","volume":"176","author":"D\u2019Avila","year":"2006","journal-title":"J Immunol"},{"key":"2023032108070696000_jlb10758-cit-0039","doi-asserted-by":"crossref","first-page":"1066","DOI":"10.1016\/j.bbalip.2009.06.004","article-title":"Lipid bodies in oxidized LDL-induced foam cells are leukotriene-synthesizing organelles: a MCP-1\/CCL2 regulated phenomenon","volume":"1791","author":"Silva","year":"2009","journal-title":"Biochim Biophys Acta"},{"key":"2023032108070696000_jlb10758-cit-0040","doi-asserted-by":"crossref","first-page":"1004","DOI":"10.1160\/TH13-01-0049","article-title":"Platelets are a previously unrecognised source of MIF","volume":"110","author":"Str\u00fc\u00dfmann","year":"2013","journal-title":"Thromb Haemost"},{"key":"2023032108070696000_jlb10758-cit-0041","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.atherosclerosis.2014.12.039","article-title":"Platelet-derived MIF: a novel platelet chemokine with distinct recruitment properties","volume":"239","author":"Wirtz","year":"2015","journal-title":"Atherosclerosis"},{"key":"2023032108070696000_jlb10758-cit-0042","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1165\/rcmb.2010-0004OC","article-title":"Cross-talk between macrophage migration inhibitory factor and eotaxin in allergic eosinophil activation forms leukotriene C\u2084-synthesizing lipid bodies","volume":"44","author":"Vieira-de-Abreu","year":"2011","journal-title":"Am J Respir Cell Mol Biol"},{"key":"2023032108070696000_jlb10758-cit-0043","doi-asserted-by":"crossref","first-page":"142","DOI":"10.4269\/ajtmh.2006.74.142","article-title":"Correlation of serum levels of macrophage migration inhibitory factor with disease severity and clinical outcome in dengue patients","volume":"74","author":"Chen","year":"2006","journal-title":"Am J Trop Med Hyg"},{"key":"2023032108070696000_jlb10758-cit-0044","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1016\/j.cyto.2011.01.013","article-title":"Macrophage migration inhibitory factor induced by dengue virus infection increases vascular permeability","volume":"54","author":"Chuang","year":"2011","journal-title":"Cytokine"},{"key":"2023032108070696000_jlb10758-cit-0045","doi-asserted-by":"crossref","first-page":"e0178820","DOI":"10.1371\/journal.pone.0178820","article-title":"Serum from dengue virus-infected patients with and without plasma leakage differentially affects endothelial cells barrier function in vitro","volume":"12","author":"Tramontini Gomes de Sousa Cardozo","year":"2017","journal-title":"PLoS One"},{"key":"2023032108070696000_jlb10758-cit-0046","doi-asserted-by":"crossref","first-page":"638","DOI":"10.4269\/ajtmh.2009.08-0008","article-title":"Hemostatic changes in Vietnamese children with mild dengue correlate with the severity of vascular leakage rather than bleeding","volume":"81","author":"Wills","year":"2009","journal-title":"Am J Trop Med Hyg"},{"key":"2023032108070696000_jlb10758-cit-0047","doi-asserted-by":"crossref","first-page":"840","DOI":"10.4269\/ajtmh.2001.65.840","article-title":"Mechanisms of hemorrhage in dengue without circulatory collapse","volume":"65","author":"Krishnamurti","year":"2001","journal-title":"Am J Trop Med Hyg"},{"key":"2023032108070696000_jlb10758-cit-0048","doi-asserted-by":"crossref","first-page":"e2412","DOI":"10.1371\/journal.pntd.0002412","article-title":"Factors associated with dengue shock syndrome: a systematic review and meta-analysis","volume":"7","author":"Huy","year":"2013","journal-title":"PLoS Negl Trop Dis"},{"key":"2023032108070696000_jlb10758-cit-0049","doi-asserted-by":"crossref","first-page":"3405","DOI":"10.1182\/blood-2013-05-504449","article-title":"Platelets mediate increased endothelium permeability in dengue through NLRP3-inflammasome activation","volume":"122","author":"Hottz","year":"2013","journal-title":"Blood"},{"key":"2023032108070696000_jlb10758-cit-0050","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1160\/TH14-01-0056","article-title":"Platelet function alterations in dengue are associated with plasma leakage","volume":"112","author":"Michels","year":"2014","journal-title":"Thromb Haemost"},{"key":"2023032108070696000_jlb10758-cit-0051","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1016\/j.trstmh.2011.10.009","article-title":"Nitric oxide activity in platelets of dengue haemorrhagic fever patients: the apparent paradoxical role of ADMA and l-NMMA","volume":"106","author":"Matsuura","year":"2012","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"2023032108070696000_jlb10758-cit-0052","doi-asserted-by":"crossref","first-page":"e1006385","DOI":"10.1371\/journal.ppat.1006385","article-title":"Platelet proteome reveals novel pathways of platelet activation and platelet-mediated immunoregulation in dengue","volume":"13","author":"Trugilho","year":"2017","journal-title":"PLoS Pathog"},{"key":"2023032108070696000_jlb10758-cit-0053","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1369\/0022155411404073","article-title":"Lipid bodies in inflammatory cells: structure, function, and current imaging techniques","volume":"59","author":"Melo","year":"2011","journal-title":"J Histochem Cytochem"},{"key":"2023032108070696000_jlb10758-cit-0054","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.pharmthera.2006.06.006","article-title":"Leukocyte lipid bodies regulation and function: contribution to allergy and host defense","volume":"113","author":"Bozza","year":"2007","journal-title":"Pharmacol Ther"},{"key":"2023032108070696000_jlb10758-cit-0055","doi-asserted-by":"crossref","first-page":"2286","DOI":"10.3389\/fmicb.2017.02286","article-title":"Modulation of lipid droplet metabolism\u2014a potential target for therapeutic intervention in flaviviridae infections","volume":"8","author":"Zhang","year":"2017","journal-title":"Front Microbiol"},{"key":"2023032108070696000_jlb10758-cit-0056","doi-asserted-by":"crossref","first-page":"e12688","DOI":"10.1111\/cmi.12688","article-title":"Lipid droplet hijacking by intracellular pathogens","volume":"19","author":"Roingeard","year":"2017","journal-title":"Cell Microbiol"},{"key":"2023032108070696000_jlb10758-cit-0057","doi-asserted-by":"crossref","first-page":"2402","DOI":"10.1038\/s41467-019-10360-4","article-title":"Extracellular vesicles from CLEC2-activated platelets enhance dengue virus-induced lethality via CLEC5A\/TLR2","volume":"10","author":"Sung","year":"2019","journal-title":"Nat Commun"},{"key":"2023032108070696000_jlb10758-cit-0058","doi-asserted-by":"crossref","first-page":"2018","DOI":"10.1182\/bloodadvances.2019001169","article-title":"Inflammatory signaling in dengue-infected platelets requires translation and secretion of nonstructural protein 1","volume":"4","author":"Quirino-Teixeira","year":"2020","journal-title":"Blood Adv"},{"key":"2023032108070696000_jlb10758-cit-0059","doi-asserted-by":"crossref","first-page":"e1007625","DOI":"10.1371\/journal.ppat.1007625","article-title":"Dengue virus nonstructural protein 1 activates platelets via Toll-like receptor 4, leading to thrombocytopenia and hemorrhage","volume":"15","author":"Chao","year":"2019","journal-title":"PLoS Pathog"},{"key":"2023032108070696000_jlb10758-cit-0060","article-title":"Innate immune receptors in platelets and platelet-leukocyte interactions","author":"Dib","journal-title":"J Leukoc Biol"},{"key":"2023032108070696000_jlb10758-cit-0061","doi-asserted-by":"crossref","first-page":"951","DOI":"10.1093\/infdis\/jir432","article-title":"Host cell lipid bodies triggered by Trypanosoma cruzi infection and enhanced by the uptake of apoptotic cells are associated with prostaglandin E2 generation and increased parasite growth","volume":"204","author":"D\u2019Avila","year":"2011","journal-title":"J Infect Dis"},{"key":"2023032108070696000_jlb10758-cit-0062","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1002\/JLB.4MR0119-035R","article-title":"Fat, fight, and beyond: the multiple roles of lipid droplets in infections and inflammation","volume":"106","author":"Pereira-Dutra","year":"2019","journal-title":"J Leukoc Biol"},{"key":"2023032108070696000_jlb10758-cit-0063","doi-asserted-by":"crossref","first-page":"1108","DOI":"10.1160\/TH07-04-0271","article-title":"Elimination of platelet factor 4 (PF4) from platelets reduces atherosclerosis in C57Bl\/6 and apoE-\/- mice","volume":"98","author":"Sachais","year":"2007","journal-title":"Thromb Haemost"},{"key":"2023032108070696000_jlb10758-cit-0064","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fphys.2012.00001","article-title":"Macrophage phenotype modulation by CXCL4 in atherosclerosis","volume":"3","author":"Gleissner","year":"2012","journal-title":"Front Physiol"}],"container-title":["Journal of Leukocyte Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2FJLB.4MA0620-658R","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/JLB.4MA0620-658R","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/JLB.4MA0620-658R","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/jleukbio\/article-pdf\/108\/4\/1293\/49458940\/jlb10758.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jleukbio\/article-pdf\/108\/4\/1293\/49458940\/jlb10758.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,3,21]],"date-time":"2023-03-21T19:44:21Z","timestamp":1679427861000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jleukbio\/article\/108\/4\/1293\/6884553"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,7,14]]},"references-count":64,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2020,7,14]]},"published-print":{"date-parts":[[2020,10,1]]}},"URL":"https:\/\/doi.org\/10.1002\/jlb.4ma0620-658r","relation":{},"ISSN":["0741-5400","1938-3673"],"issn-type":[{"value":"0741-5400","type":"print"},{"value":"1938-3673","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2020,10]]},"published":{"date-parts":[[2020,7,14]]},"assertion":[{"value":"2020-02-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-06-28","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-07-14","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}