{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T07:15:02Z","timestamp":1771485302813,"version":"3.50.1"},"reference-count":37,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2019,2,12]],"date-time":"2019-02-12T00:00:00Z","timestamp":1549929600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["NIH R21AI119634"],"award-info":[{"award-number":["NIH R21AI119634"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"name":"University of Missouri College of Veterinary Medicine and Research Board"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,6,27]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Brucellosis, caused by the intracellular bacterial pathogen Brucella, is a globally important zoonotic disease for which arthritis is the most common focal complication in humans. Wild-type mice infected systemically with Brucella typically do not exhibit arthritis, but mice lacking IFN-\u03b3 develop arthritis regardless of the route of Brucella infection. Here, we investigated mechanisms by which IFN-\u03b3 suppresses Brucella-induced arthritis. Several cell types, including innate lymphoid cells, contributed to IFN-\u03b3 production and suppression of joint swelling. IFN-\u03b3 deficiency resulted in elevated joint IL-1\u03b2 levels, and severe joint inflammation that was entirely inflammasome dependent, and in particular, reliant on the NLRP3 inflammasome. IFN-\u03b3 was vital for induction of the nitric oxide producing enzyme, iNOS, in infected joints, and nitric oxide directly inhibited IL-1\u03b2 production and inflammasome activation in Brucella-infected macrophages in vitro. During in vivo infection, iNOS deficiency resulted in an increase in IL-1\u03b2 and inflammation in Brucella-infected joints. Collectively, this data indicate that IFN-\u03b3 prevents arthritis both by limiting Brucella infection, and by inhibiting excessive inflammasome activation through the induction of nitric oxide.<\/jats:p>","DOI":"10.1002\/jlb.4mia1018-409r","type":"journal-article","created":{"date-parts":[[2019,2,12]],"date-time":"2019-02-12T18:42:56Z","timestamp":1549996976000},"page":"27-34","source":"Crossref","is-referenced-by-count":14,"title":["IFN-\u03b3-dependent nitric oxide suppresses <i>Brucella<\/i>-induced arthritis by inhibition of inflammasome activation"],"prefix":"10.1093","volume":"106","author":[{"given":"Carolyn A","family":"Lacey","sequence":"first","affiliation":[{"name":"Department of Veterinary Pathobiology, University of Missouri , Columbia, Missouri, USA"},{"name":"Laboratory for Infectious Disease Research, University of Missouri , Columbia, Missouri, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine A","family":"Chambers","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology, University of Missouri , Columbia, Missouri, USA"},{"name":"Laboratory for Infectious Disease Research, University of Missouri , Columbia, Missouri, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William J","family":"Mitchell","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology, University of Missouri , Columbia, Missouri, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jerod A","family":"Skyberg","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology, University of Missouri , Columbia, Missouri, USA"},{"name":"Laboratory for Infectious Disease Research, University of Missouri , Columbia, Missouri, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2019,2,12]]},"reference":[{"key":"2023032406371461000_jlb10345-cit-0001","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/S1473-3099(06)70382-6","article-title":"The new global map of human brucellosis?","volume":"6","author":"Pappas","year":"2006","journal-title":"Lancet Infect Dis"},{"key":"2023032406371461000_jlb10345-cit-0002","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1136\/ard.50.1.23","article-title":"Osteoarticular complications of brucellosis?","volume":"50","author":"Colmenero","year":"1991","journal-title":"Ann Rheum Dis"},{"key":"2023032406371461000_jlb10345-cit-0003","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.prevetmed.2005.01.017","article-title":"A model of animal\u2013human brucellosis transmission in Mongolia?","volume":"69","author":"Zinsstag","year":"2005","journal-title":"Prev Vet Med"},{"key":"2023032406371461000_jlb10345-cit-0004","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1111\/j.1749-6632.1980.tb18912.x","article-title":"Airborne spread of brucellosis?","volume":"353","author":"Kaufmann","year":"1980","journal-title":"Ann NY Acad Sci"},{"key":"2023032406371461000_jlb10345-cit-0005","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/S0950-3579(05)80153-0","article-title":"Bacterial infections: osteoarticular brucellosis?","volume":"9","author":"Rajapakse","year":"1995","journal-title":"Bailliere\u2019s Clin Rheum"},{"key":"2023032406371461000_jlb10345-cit-0006","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/0049-0172(82)90064-6","article-title":"Articular involvement in human brucellosis: a retrospective analysis of 304 cases?","volume":"112","author":"Gotuzzo","year":"1982","journal-title":"Semin Arthritis Rheum."},{"key":"2023032406371461000_jlb10345-cit-0007","article-title":"Arthroscopic synovectomy, an alternative in the treatment of brucellar arthritis of the knee with prolonged course. A report of two cases?","volume":"13","author":"Martin-Hernandez","year":"2009","journal-title":"Int J Orthopedic Surg"},{"key":"2023032406371461000_jlb10345-cit-0008","doi-asserted-by":"crossref","first-page":"994","DOI":"10.1136\/ard.49.12.994","article-title":"Brucella arthritis: a study of 96 cases in Kuwait?","volume":"49","author":"Khateeb","year":"1990","journal-title":"Ann Rheum Dis"},{"key":"2023032406371461000_jlb10345-cit-0009","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1007\/s10067-002-8283-6","article-title":"Leukocyte count in the synovial fluid of children with culture-proven brucellar arthritis?","volume":"21","author":"Press","year":"2002","journal-title":"Clin Rheum"},{"key":"2023032406371461000_jlb10345-cit-0010","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1007\/s00296-004-0453-1","article-title":"A case of brucellar septic arthritis of the knee with a prolonged clinical 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murine macrophages?","volume":"66","author":"Gross","year":"1998","journal-title":"Infect Immun"},{"key":"2023032406371461000_jlb10345-cit-0035","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1002\/jlb.58.6.643","article-title":"On the expression of nitric oxide synthase by human macrophages. Why no NO?","volume":"58","author":"Albina","year":"1995","journal-title":"J Leukoc Biol"},{"key":"2023032406371461000_jlb10345-cit-0036","doi-asserted-by":"crossref","first-page":"e003493","DOI":"10.1161\/JAHA.116.003493","article-title":"Discovery and clinical evaluation of MK-8150, a novel nitric oxide donor with a unique mechanism of nitric oxide release?","volume":"5","author":"Knox","year":"2016","journal-title":"J Am Heart Assoc"},{"key":"2023032406371461000_jlb10345-cit-0037","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1006\/cimm.1993.1241","article-title":"Macrophage control of Brucella abortus: role of reactive oxygen intermediates and nitric oxide?","volume":"151","author":"Jiang","year":"1993","journal-title":"Cell Immunol"}],"container-title":["Journal of Leukocyte Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2FJLB.4MIA1018-409R","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/jleukbio\/article-pdf\/106\/1\/27\/49485553\/jlb10345.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jleukbio\/article-pdf\/106\/1\/27\/49485553\/jlb10345.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,3,24]],"date-time":"2023-03-24T06:56:16Z","timestamp":1679640976000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jleukbio\/article\/106\/1\/27\/6935666"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,2,12]]},"references-count":37,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2019,2,12]]},"published-print":{"date-parts":[[2019,6,27]]}},"URL":"https:\/\/doi.org\/10.1002\/jlb.4mia1018-409r","relation":{},"ISSN":["1938-3673","0741-5400"],"issn-type":[{"value":"1938-3673","type":"electronic"},{"value":"0741-5400","type":"print"}],"subject":[],"published-other":{"date-parts":[[2019,7]]},"published":{"date-parts":[[2019,2,12]]}}}