{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T12:13:12Z","timestamp":1770725592261,"version":"3.49.0"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[1992,1,1]],"date-time":"1992-01-01T00:00:00Z","timestamp":694224000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1992,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The visible excitation and emission wavelengths of the recently developed fluorescent Ca2+ indicator fluo-3 permit analysis of the intracellular Ca2+ concentration, [Ca2+]i, in flow cytometry with a 488-nm argon laser. The role of [Ca2+]i in human polymorphonuclear leukocyte heterogeneity was investigated in response to formyl-methionyl-leucyl-phenylalanine (fMLP), C5a, and interleukin 8\/neutrophil attractant\/activation protein 1 (IL-8\/NAP-1) by flow cytometry. The [Ca2+]i changes in different subpopulations within a heterogeneous cell suspension were resolved upon stimulation with fMLP. Using an anti-CD16 phycoerythrin-conjugated antibody and fluo-3 simultaneously, neutrophils affected and nonaffected in Ca2+ mobilization were distinguished in two patients suffering from glycogen storage disease type lb. In normal neutrophils, a different time course of Ca2+ mobilization of neutrophil subpopulations immediately after stimulation with fMLP was detected. In addition, after stimulation with a low concentration of IL-8\/NAP-1 (10-10 M) two subsets of neutrophils appeared; one of them showed an increase in [Ca2+]i, while the other did not. These results indicate heterogeneity in the neutrophil signal transduction process involved in Ca2+ mobilization. Therefore, flow cytometric analyses can resolve changes in single-cell [Ca2+]i distribution patterns, which is important for the understanding of [Ca2+]i in neutrophil heterogeneous activation processes.<\/jats:p>","DOI":"10.1002\/jlb.51.1.77","type":"journal-article","created":{"date-parts":[[2018,1,12]],"date-time":"2018-01-12T19:48:39Z","timestamp":1515786519000},"page":"77-83","source":"Crossref","is-referenced-by-count":30,"title":["Heterogeneity in the mobilization of cytoplasmic calcium by human polymorphonuclear leukocytes in response to fMLP, C5a and IL-8\/NAP-1"],"prefix":"10.1093","volume":"51","author":[{"given":"J\u00f6rn","family":"Eisner","sequence":"first","affiliation":[{"name":"Department of Immunobiology, Fraunhofer Institut ITA , Hannover, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Volkhard","family":"Kaever","sequence":"additional","affiliation":[{"name":"Division of Molecular Pharmacology, Department of Pharmacology and Toxicology, Hannover Medical School , Hannover, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andreas","family":"Emmend\u00f6rffer","sequence":"additional","affiliation":[{"name":"Department of Immunobiology, Fraunhofer Institut ITA , Hannover, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Breidenbach","sequence":"additional","affiliation":[{"name":"Department of Immunobiology, Fraunhofer Institut ITA , Hannover, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marie-Luise","family":"Lohmann-Matthes","sequence":"additional","affiliation":[{"name":"Department of Immunobiology, Fraunhofer Institut ITA , Hannover, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joachim","family":"Roesler","sequence":"additional","affiliation":[{"name":"Department of Immunobiology, Fraunhofer Institut ITA , Hannover, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[1992,1,1]]},"container-title":["Journal of Leukocyte Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjlb.51.1.77","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/jleukbio\/article-pdf\/51\/1\/77\/49768197\/jlb0077.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jleukbio\/article-pdf\/51\/1\/77\/49768197\/jlb0077.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,11]],"date-time":"2023-04-11T07:50:16Z","timestamp":1681199416000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jleukbio\/article\/51\/1\/77\/6977314"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,1,1]]},"references-count":0,"journal-issue":{"issue":"1","published-online":{"date-parts":[[1992,1,1]]},"published-print":{"date-parts":[[1992,1,1]]}},"URL":"https:\/\/doi.org\/10.1002\/jlb.51.1.77","relation":{},"ISSN":["0741-5400","1938-3673"],"issn-type":[{"value":"0741-5400","type":"print"},{"value":"1938-3673","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[1992,1]]},"published":{"date-parts":[[1992,1,1]]}}}