{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T10:13:16Z","timestamp":1770718396148,"version":"3.49.0"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[1993,7,1]],"date-time":"1993-07-01T00:00:00Z","timestamp":741484800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1993,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>We examined leukemic cells, HL-60, an acute promyelocytic leukemia cell line, after differentiation induced by 1,25-dihydroxyvitamin D3 (D3) and retinoic acid (A) for infection of Legionella pneumophila, the etiologic agent of Legionnaires\u2019 disease. We investigated the effect of interferon \u03b3 (IFN-\u03b3) on the differentiated cells and on the intracellular growth of the bacteria. An examination of morphological and antigenic changes in the cells was also included in the study. After 4-day incubation with 10-6M D3 or A, the HL-60 cells differentiated into monocyte-like (D3-HL-60) or mature granulocyte-like (A-HL-60) cells, respectively. They were then infected with L. pneumophila. Intracellular multiplication of the bacteria was evident in D3-HL-60 cells but not in HL-60 or A-HL-60 cells. D3-HL-60 cells required a 24-h infection time for the intracellular growth of L. pneumophila. D3-HL-60 cells activated with human recombinant IFN-7 for 1-24 h (\u03b3-IFN-D3-HL-60 cells) before infection markedly inhibited L. pneumophila multiplication, the effect of IFN-\u03b3 being dose dependent. Surface marker analysis was carried out in HL-60, D3-HL-60, and 7-IFN-D3-HL-60 cells. On D3-HL-60 cells, CDllb, CDllc, CD14, and CD35 antigen increased, whereas CD71 and HLA-DR antigen decreased. This finding suggested that HL-60 cells differentiated into monocyte-like cells; the acquisition of the complement receptors, CDllb(CR3) and CD35(CR1), seemed to be important for phagocytosis and for the subsequent intracellular multiplication of L. pneumophila. The \u03b3-IFN- D3-HL-6O cells showed an increase of CD16, CD36, CD71, and HLA-DR antigen, suggesting that they were in an activated state. Our study indicated, first, that D3 can induce human leukemic cells to differentiate into functional monocyte-macrophage-like cells that can support the intracellular multiplication of L. pneumophila and, second, that these differentiated leukemic cells can be activated by IFN-\u03b3 to markedly inhibit bacterial growth.<\/jats:p>","DOI":"10.1002\/jlb.54.1.40","type":"journal-article","created":{"date-parts":[[2018,1,12]],"date-time":"2018-01-12T19:17:27Z","timestamp":1515784647000},"page":"40-46","source":"Crossref","is-referenced-by-count":19,"title":["Intracellular multiplication of Legionella pneumophila in HL-60 cells differentiated by 1,25-dihydroxyvitamin D3 and the effect of interferon y"],"prefix":"10.1093","volume":"54","author":[{"given":"Masayuki","family":"Watanabe","sequence":"first","affiliation":[{"name":"Department of Internal Medicine and Microbiology; Saga Medical School, Saga, and Department of Microbiology, School of Medicine, University of Occupational and Environmental Health , Kitakyushu , Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshinori","family":"Shimamoto","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine and Microbiology; Saga Medical School, Saga, and Department of Microbiology, School of Medicine, University of Occupational and Environmental Health , Kitakyushu , Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shin-ichi","family":"Yoshida","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine and Microbiology; Saga Medical School, Saga, and Department of Microbiology, School of Medicine, University of Occupational and Environmental Health , Kitakyushu , Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenji","family":"Suga","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine and Microbiology; Saga Medical School, Saga, and Department of Microbiology, School of Medicine, University of Occupational and Environmental Health , Kitakyushu , Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuo","family":"Mizuguchi","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine and Microbiology; Saga Medical School, Saga, and Department of Microbiology, School of Medicine, University of Occupational and Environmental Health , Kitakyushu , Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Osamu","family":"Kohashi","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine and Microbiology; Saga Medical School, Saga, and Department of Microbiology, School of Medicine, University of Occupational and Environmental Health , Kitakyushu , Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masaya","family":"Yamaguchi","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine and Microbiology; Saga Medical School, Saga, and Department of Microbiology, School of Medicine, University of Occupational and Environmental Health , Kitakyushu , Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[1993,7,1]]},"container-title":["Journal of Leukocyte Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjlb.54.1.40","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/jleukbio\/article-pdf\/54\/1\/40\/49769444\/jlb0040.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jleukbio\/article-pdf\/54\/1\/40\/49769444\/jlb0040.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,11]],"date-time":"2023-04-11T06:48:48Z","timestamp":1681195728000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jleukbio\/article\/54\/1\/40\/6978640"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,7,1]]},"references-count":0,"journal-issue":{"issue":"1","published-online":{"date-parts":[[1993,7,1]]},"published-print":{"date-parts":[[1993,7,1]]}},"URL":"https:\/\/doi.org\/10.1002\/jlb.54.1.40","relation":{},"ISSN":["0741-5400","1938-3673"],"issn-type":[{"value":"0741-5400","type":"print"},{"value":"1938-3673","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[1993,7]]},"published":{"date-parts":[[1993,7,1]]}}}