{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,30]],"date-time":"2025-04-30T05:48:34Z","timestamp":1745992114525},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[1994,5,1]],"date-time":"1994-05-01T00:00:00Z","timestamp":767750400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1994,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Atherosclerotic lesions contain multiple cell types including smooth muscle cells, macrophages, and T lymphocytes. The development of an extralymphatic T lymphocyte focus of inflammation in this condition requires chemoattractant-induced cell migration and growth factor-induced cell activation. In a previous study, we described a novel 13\u201315-kDa T lymphocyte-specific chemotactic cytokine, endothelial cell\u2013derived lymphocyte chemoattractant activity (ED-LCA), secreted by serotonin-stimulated bovine aortic endothelial cells that is distinct from previously identified endothelial cell\u2013derived interleukins (IL) 1, 6, and 8. Because of the association between T lymphocyte chemotactic and growth factor activity, in the current study we investigated the effect of ED-LCA on T cell growth. We assessed its capacity to induce markers of the passage of T cells from the resting (G0) state into the G1 phase of the cell cycle, such as receptors for IL-2 (IL-2R) and transferrin (TFR) and class II major histocompatibility complex antigens (HLA-DR). Incubation of G0 freshly isolated human T lymphocytes for 48 h with chromatographically resolved, partially purified ED-LCA resulted in a threefold increase in expression of the p55 subunit of IL-2R, a threefold increase in TFR, and a twofold increase in HLA-DR. Passage into the G1 phase of the cell cycle was confirmed by cell cycle analysis employing acridine orange. Evaluation of CD4+ and CD8+ T cell subsets by double-antibody labeling demonstrated that the p55 subunit of IL-2R was induced in both T cell subsets. Although incubation of human T cells with ED-LCA alone did not induce proliferation, addition of exogenous IL-2 to T cells pulsed with ED-LCA for 24 h caused a proliferative response with a stimulation index of 3. By up-regulating functional cell surface receptors for IL-2, ED-LCA is a competence growth factor for T lymphocytes and primes them to respond to IL-2. By virtue of its effect on T cells, as a chemotactic and competence factor, this endothelial cell-derived mitoattractant could participate with other T cell growth factors like IL-2 in the recruitment and amplification of the extralymphatic T cell component of atherosclerosis. J. Leukoc, Biol. 55: 567\u2013573; 1994.<\/jats:p>","DOI":"10.1002\/jlb.55.5.567","type":"journal-article","created":{"date-parts":[[2018,1,12]],"date-time":"2018-01-12T19:42:57Z","timestamp":1515786177000},"page":"567-573","source":"Crossref","is-referenced-by-count":23,"title":["Serotonin-stimulated aortic endothelial cells secrete a novel T lymphocyte chemotactic and growth factor"],"prefix":"10.1093","volume":"55","author":[{"given":"Marcia F","family":"Katz","sequence":"first","affiliation":[{"name":"Pulmonary Center, Boston University School of Medicine , Boston, Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harrison W","family":"Farber","sequence":"additional","affiliation":[{"name":"Pulmonary Center, Boston University School of Medicine , Boston, Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zoe","family":"Dodds-Stitt","sequence":"additional","affiliation":[{"name":"Pulmonary Center, Boston University School of Medicine , Boston, Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William W","family":"Cruikshank","sequence":"additional","affiliation":[{"name":"Pulmonary Center, Boston University School of Medicine , Boston, Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dennis J","family":"Bear","sequence":"additional","affiliation":[{"name":"Pulmonary Center, Boston University School of Medicine , Boston, Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[1994,5,1]]},"container-title":["Journal of Leukocyte Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjlb.55.5.567","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/jleukbio\/article-pdf\/55\/5\/567\/49770652\/jlb0567.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jleukbio\/article-pdf\/55\/5\/567\/49770652\/jlb0567.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,11]],"date-time":"2023-04-11T06:34:06Z","timestamp":1681194846000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jleukbio\/article\/55\/5\/567\/6978682"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,5,1]]},"references-count":0,"journal-issue":{"issue":"5","published-online":{"date-parts":[[1994,5,1]]},"published-print":{"date-parts":[[1994,5,1]]}},"URL":"https:\/\/doi.org\/10.1002\/jlb.55.5.567","relation":{},"ISSN":["0741-5400","1938-3673"],"issn-type":[{"value":"0741-5400","type":"print"},{"value":"1938-3673","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[1994,5]]},"published":{"date-parts":[[1994,5,1]]}}}