{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T11:29:54Z","timestamp":1791890994572,"version":"4.3.4"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[1994,8,1]],"date-time":"1994-08-01T00:00:00Z","timestamp":775699200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1994,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>In this study we investigated the involvement of inflammatory cells in the pleural accumulation of eosinophils induced by lipopolysaccharide (LPS). Intrathoracic (i.t.) injection of LPS (250 ng\/cavity) into rats induced a significant eosinophil accumulation that developed within 24 h, was maximal at 48 h, and returned to control values within 120 h. This eosinophil influx was preceded by a huge neutrophil influx within 4 h and accompanied by a mononuclear cell accumulation between 24 and 48 h. Pretreatment with an antineutrophil monoclonal antibody (RP-3, 2 ml per animal) selectively reduced the number of circulating neutrophils within 8 h but failed to alter the LPS-induced eosinophilia. Similarly, platelet depletion with an anti-rat platelet antiserum did not alter the LPS-induced eosinophil accumulation. Cyclosporine (50 mg\/kg, 12 and 2 h before) partially inhibited (51%) the LPS-induced pleural eosinophilia, whereas the eosinophilia was not changed by prior degranulation of pleural mast cells with polymyxin B (10 \u03bcg\/cavity, 24 h before). Moreover, selective depletion of T lymphocytes using an anti-Thy 1.0 monoclonal antibody significantly inhibited the eosinophilia triggered by LPS. The i.t. injection of liposomes containing dichloromethylene diphosphonate significantly reduced (65%) the number of resident macrophages after 5 days. Under this condition, the eosinophil infiltration induced by LPS was completely inhibited. Accordingly, the i.t. injection of supernatant from macrophage monolayers, obtained from the pleural cavities of LPS-injected rats, into naive recipient animals led to a twofold increase in the number of pleural eosinophils. In conclusion, our data suggest an important role for resident macrophages and T lymphocytes in the eosinophil accumulation induced by LPS. J. Leukoc. Biol. 56: 151\u2013158; 1994.<\/jats:p>","DOI":"10.1002\/jlb.56.2.151","type":"journal-article","created":{"date-parts":[[2018,1,12]],"date-time":"2018-01-12T19:41:36Z","timestamp":1515786096000},"page":"151-158","source":"Crossref","is-referenced-by-count":19,"title":["Requirement for lymphocytes and resident macrophages in LPS-induced pleural eosinophil accumulation"],"prefix":"10.1093","volume":"56","author":[{"given":"Patricia T","family":"Bozza","sequence":"first","affiliation":[{"name":"Departamento de Fisiologia e Farmacodin\u00e2mica and Institute Oswaldo Cruz\/FIOCRUZ , Rio de Janeiro , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hugo C","family":"Castro-Faria-Neto","sequence":"additional","affiliation":[{"name":"Departamento de Fisiologia e Farmacodin\u00e2mica and Institute Oswaldo Cruz\/FIOCRUZ , Rio de Janeiro , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"Penido","sequence":"additional","affiliation":[{"name":"Departamento de Fisiologia e Farmacodin\u00e2mica and Institute Oswaldo Cruz\/FIOCRUZ , Rio de Janeiro , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9a P","family":"Larangeira","sequence":"additional","affiliation":[{"name":"Departamento de Fisiologia e Farmacodin\u00e2mica and Institute Oswaldo Cruz\/FIOCRUZ , Rio de Janeiro , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria das","family":"Gra\u00e7as","sequence":"additional","affiliation":[{"name":"Departamento de Fisiologia e Farmacodin\u00e2mica and Institute Oswaldo Cruz\/FIOCRUZ , Rio de Janeiro , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M O","family":"Henriques","sequence":"additional","affiliation":[{"name":"Departamento de Fisiologia e Farmacodin\u00e2mica and Institute Oswaldo Cruz\/FIOCRUZ , Rio de Janeiro , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia M R","family":"Silva","sequence":"additional","affiliation":[{"name":"Departamento de Fisiologia e Farmacodin\u00e2mica and Institute Oswaldo Cruz\/FIOCRUZ , Rio de Janeiro , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco A","family":"Martins","sequence":"additional","affiliation":[{"name":"Departamento de Fisiologia e Farmacodin\u00e2mica and Institute Oswaldo Cruz\/FIOCRUZ , Rio de Janeiro , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo Ribeiro","family":"dos Santos","sequence":"additional","affiliation":[{"name":"Departamento de Fisiologia e Farmacodin\u00e2mica and Institute Oswaldo Cruz\/FIOCRUZ , Rio de Janeiro , Brazil"},{"name":"Departamento de Bioquimica e Biologia Molecular, Institute Oswaldo Cruz\/FIOCRUZ , Rio de Janeiro , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renato S B","family":"Cordeiro","sequence":"additional","affiliation":[{"name":"Departamento de Fisiologia e Farmacodin\u00e2mica and Institute Oswaldo Cruz\/FIOCRUZ , Rio de Janeiro , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[1994,8,1]]},"container-title":["Journal of Leukocyte Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjlb.56.2.151","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/jleukbio\/article-pdf\/56\/2\/151\/49771191\/jlb0151.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jleukbio\/article-pdf\/56\/2\/151\/49771191\/jlb0151.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,11]],"date-time":"2023-04-11T05:21:55Z","timestamp":1681190515000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jleukbio\/article\/56\/2\/151\/6978878"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,8,1]]},"references-count":0,"journal-issue":{"issue":"2","published-online":{"date-parts":[[1994,8,1]]},"published-print":{"date-parts":[[1994,8,1]]}},"URL":"https:\/\/doi.org\/10.1002\/jlb.56.2.151","relation":{},"ISSN":["0741-5400","1938-3673"],"issn-type":[{"value":"0741-5400","type":"print"},{"value":"1938-3673","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[1994,8]]},"published":{"date-parts":[[1994,8,1]]}}}