{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T16:03:15Z","timestamp":1772899395345,"version":"3.50.1"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[1995,10,1]],"date-time":"1995-10-01T00:00:00Z","timestamp":812505600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1995,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Eosinophils are supposed to play a critical role in the pathology of several allergic diseases because after activation they can release toxic and proinflammatory agents. In this study we have investigated whether IgE-mediated rat pleurisy could be affected by an ongoing pleural eosinophilic inflammatory response. IgE-passively sensitized rats were challenged with an intrapleural (i.pl.) injection of allergen (dinitrophenylated bovine serum albumin, 1 \u03bcg\/cavity) and exudation assessed by measuring the amount of protein extravasated into the pleural cavity within 4 h. We have confirmed that lipopolysaccharide (LPS) stimulation (250 ng\/cavity i.pl.) was followed by a marked pleural neutrophilia, apparent at 3 h, which was followed by an eosinophil accumulation noted within 48\u201372 h postchallenge. We have also confirmed that a boiled sample of LPS pleural washing (IPS-PW, 200 \u03bcl i.pl.) caused selective eosinophilia in recipient rats. Pleural exudation remained unaltered when the allergenic challenge was performed 3 h after IPS in a condition of intense pleural fluid neutrophilia. In contrast, this was significantly reduced (P&amp;lt; .001) when the challenge occurred 72 h after IPS or 24 h after IPS-PW in selective pleural fluid eosinophilia. In another series of experiments repeated daily i.pl. injections of platelet-activating factor (PAF; 1 \u03bcg\/cavity) resulted in a progressive increase in eosinophil number recovered from the pleural cavity. The values were 1.2 \u00b1 0.2, 3.0 \u00b1 0.2, and 5.8 \u00b1 0.5 \u00d7 106 eosinophils\/cavity (mean\u00b1SEM) after 0, 1, and 4 injections, respectively. Allergen challenge performed after 0, 1, or 4 PAF stimulations led to pleural protein levels of 88.6 \u00b1 5.7, 33.7 \u00b1 0.7, and 19.4 \u00b1 2.3 mg\/cavity, respectively, indicating that the allergic pleurisy is inhibited in a manner dependent on the magnitude of eosinophil accumulation. Furthermore, the impairment of PAF-induced eosinophil accumulation by cetirizine (30 mg\/kg i.p.) restored the exudatory response. Exudation triggered by compound 48\/80 (25 \u03bcg\/cavity), histamine (200 \u03bcg\/cavity), or 5-hydroxytryptamine (100 \u03bcg\/cavity) was not affected by four previous PAF daily injections. The findings indicate that allergen-induced exudation is selectively down-regulated in the eosinophil-enriched pleural space of rats, a suppression that increased with increasing eosinophil number and disappeared after chemical impairment of the eosinophilia.<\/jats:p>","DOI":"10.1002\/jlb.58.4.395","type":"journal-article","created":{"date-parts":[[2018,1,12]],"date-time":"2018-01-12T19:06:26Z","timestamp":1515783986000},"page":"395-402","source":"Crossref","is-referenced-by-count":6,"title":["Pleural fluid eosinophils suppress local IgE-mediated protein exudation in rats"],"prefix":"10.1093","volume":"58","author":[{"given":"Christianne","family":"Bandeira-Melo","sequence":"first","affiliation":[{"name":"Departamento de Fisiologia e Farmacodinamica, Oswaldo Cruz Institute , Rio de Janeiro , Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia M R e","family":"Silva","sequence":"additional","affiliation":[{"name":"Departamento de Fisiologia e Farmacodinamica, Oswaldo Cruz Institute , Rio de Janeiro , Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renato S B","family":"Cordeiro","sequence":"additional","affiliation":[{"name":"Departamento de Fisiologia e Farmacodinamica, Oswaldo Cruz Institute , Rio de Janeiro , Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marco A","family":"Martins","sequence":"additional","affiliation":[{"name":"Departamento de Fisiologia e Farmacodinamica, Oswaldo Cruz Institute , Rio de Janeiro , Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[1995,10,1]]},"container-title":["Journal of Leukocyte Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjlb.58.4.395","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/jleukbio\/article-pdf\/58\/4\/395\/49764068\/jlb0395.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jleukbio\/article-pdf\/58\/4\/395\/49764068\/jlb0395.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,12]],"date-time":"2023-04-12T07:11:34Z","timestamp":1681283494000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jleukbio\/article\/58\/4\/395\/6979042"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,10,1]]},"references-count":0,"journal-issue":{"issue":"4","published-online":{"date-parts":[[1995,10,1]]},"published-print":{"date-parts":[[1995,10,1]]}},"URL":"https:\/\/doi.org\/10.1002\/jlb.58.4.395","relation":{},"ISSN":["0741-5400","1938-3673"],"issn-type":[{"value":"0741-5400","type":"print"},{"value":"1938-3673","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[1995,10]]},"published":{"date-parts":[[1995,10,1]]}}}