{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T22:18:51Z","timestamp":1791843531452,"version":"4.3.4"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[1995,11,1]],"date-time":"1995-11-01T00:00:00Z","timestamp":815184000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1995,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Only Listeria monocytogenes that produce listeriolysin O (LLO) elicit protective immunity. Given the importance of tumor necrosis factor \u03b1 (TNF-\u03b1) in anti-Listeria immunity, we have investigated TNF-\u03b1 production by macrophages after they ingested live LLO-producing compared to LLO-non-producing bacteria. We used two genetically engineered strains of Listeria that differed only in their ability (Ly+) or inability (Ly-) to produce LLO. Ly+ and Ly- caused the same kinetics of increased mRNA abundance for TNF-\u03b1 during the first 90 min after phagocytosis. However, only Ly+ caused sustained transcription of TNF-\u03b1 mRNA, and this may account for the increased release of TNF-\u03b1. The transcriptional inhibitor 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB) prevented the sustained abundance of cytokine mRNA 20 h after ingestion of Ly+. In addition, nuclear run-on assays indicated sustained transcription of TNF-\u03b1 genes only after ingestion of Ly+. LLO itself was not responsible for the ability of Ly+ to stimulate the sustained transcription of the TNF-\u03b1 genes. Instead, LLO may allow Listeria to survive within macrophages so that other bacterial products cause sustained TNF-\u03b1 gene transcription. Both Ly+ and Ly- produced molecules, isolated by 50% ammonium sulfate, that induced cytokine production. In conclusion, we now report that Ly+ causes sustained transcription of the TNF-\u03b1 gene and production of TNF-\u03b1 by macrophages in vitro. We speculate that the TNF-\u03b1 may activate endothelium and thus allow the recruitment of T cells to sites of infection. This may contribute to the ability of only LLO-producing Listeria to induce protective immunity.<\/jats:p>","DOI":"10.1002\/jlb.58.5.556","type":"journal-article","created":{"date-parts":[[2018,1,12]],"date-time":"2018-01-12T19:06:58Z","timestamp":1515784018000},"page":"556-562","source":"Crossref","is-referenced-by-count":15,"title":["Differential regulation of TNF-\u03b1 production by listeriolysin-producing versus nonproducing strains of Listeria monocytogenes"],"prefix":"10.1093","volume":"58","author":[{"given":"M A","family":"Vazquez","sequence":"first","affiliation":[{"name":"Graduate Program in Immunology, Division of Cell and Molecular Biology; and Division of Nephrology, Department of Internal Medicine, University of Texas, Southwestern Medical School , Dallas, Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S C","family":"Sicher","sequence":"additional","affiliation":[{"name":"Graduate Program in Immunology, Division of Cell and Molecular Biology; and Division of Nephrology, Department of Internal Medicine, University of Texas, Southwestern Medical School , Dallas, Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W J","family":"Wright","sequence":"additional","affiliation":[{"name":"Graduate Program in Immunology, Division of Cell and Molecular Biology; and Division of Nephrology, Department of Internal Medicine, University of Texas, Southwestern Medical School , Dallas, Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M L","family":"Proctor","sequence":"additional","affiliation":[{"name":"Graduate Program in Immunology, Division of Cell and Molecular Biology; and Division of Nephrology, Department of Internal Medicine, University of Texas, Southwestern Medical School , Dallas, Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S R","family":"Schmalzried","sequence":"additional","affiliation":[{"name":"Graduate Program in Immunology, Division of Cell and Molecular Biology; and Division of Nephrology, Department of Internal Medicine, University of Texas, Southwestern Medical School , Dallas, Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K R","family":"Stallworth","sequence":"additional","affiliation":[{"name":"Graduate Program in Immunology, Division of Cell and Molecular Biology; and Division of Nephrology, Department of Internal Medicine, University of Texas, Southwestern Medical School , Dallas, Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J C","family":"Crowley","sequence":"additional","affiliation":[{"name":"Graduate Program in Immunology, Division of Cell and Molecular Biology; and Division of Nephrology, Department of Internal Medicine, University of Texas, Southwestern Medical School , Dallas, Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C Y","family":"Lu","sequence":"additional","affiliation":[{"name":"Graduate Program in Immunology, Division of Cell and Molecular Biology; and Division of Nephrology, Department of Internal Medicine, University of Texas, Southwestern Medical School , Dallas, Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[1995,11,1]]},"container-title":["Journal of Leukocyte Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjlb.58.5.556","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/jleukbio\/article-pdf\/58\/5\/556\/49764016\/jlb0556.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jleukbio\/article-pdf\/58\/5\/556\/49764016\/jlb0556.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,12]],"date-time":"2023-04-12T07:03:46Z","timestamp":1681283026000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jleukbio\/article\/58\/5\/556\/6979135"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,11,1]]},"references-count":0,"journal-issue":{"issue":"5","published-online":{"date-parts":[[1995,11,1]]},"published-print":{"date-parts":[[1995,11,1]]}},"URL":"https:\/\/doi.org\/10.1002\/jlb.58.5.556","relation":{},"ISSN":["0741-5400","1938-3673"],"issn-type":[{"value":"0741-5400","type":"print"},{"value":"1938-3673","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[1995,11]]},"published":{"date-parts":[[1995,11,1]]}}}