{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:46:53Z","timestamp":1786978013193,"version":"build-2736575974"},"reference-count":29,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2020,1,22]],"date-time":"2020-01-22T00:00:00Z","timestamp":1579651200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"},{"start":{"date-parts":[[2020,1,22]],"date-time":"2020-01-22T00:00:00Z","timestamp":1579651200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2020,1,22]],"date-time":"2020-01-22T00:00:00Z","timestamp":1579651200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001659","name":"DFG","doi-asserted-by":"publisher","award":["WI 5021-21 \u2013 FOR2799"],"award-info":[{"award-number":["WI 5021-21 \u2013 FOR2799"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007316","name":"Klaus Tschira Foundation","doi-asserted-by":"publisher","award":["00.316.2017"],"award-info":[{"award-number":["00.316.2017"]}],"id":[{"id":"10.13039\/501100007316","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009397","name":"Medical Faculty of the University of T\u00fcbingen","doi-asserted-by":"crossref","award":["2509-0-0"],"award-info":[{"award-number":["2509-0-0"]}],"id":[{"id":"10.13039\/501100009397","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["WI 5021\u201021 \u2013 FOR2799"],"award-info":[{"award-number":["WI 5021\u201021 \u2013 FOR2799"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007316","name":"Klaus Tschira Stiftung","doi-asserted-by":"publisher","award":["00.316.2017"],"award-info":[{"award-number":["00.316.2017"]}],"id":[{"id":"10.13039\/501100007316","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2020,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Dissection of the role and function of human \u03b3\u03b4 T cells and their heterogeneous subsets in cancer, inflammation, and auto-immune diseases is a growing and dynamic research field of increasing interest to the scientific community. Therefore, harmonization and standardization of techniques for the characterization of peripheral and tissue-resident \u03b3\u03b4 T cells is crucial to facilitate comparability between published and emerging research. The application of commercially available reagents to classify \u03b3\u03b4 T cells, in particular the combination of multiple Abs, is not always trouble-free, posing major demands on researchers entering this field. Occasionally, even entire \u03b3\u03b4 T cell subsets may remain undetected when certain Abs are combined in flow cytometric analysis with multicolor Ab panels, or might be lost during cell isolation procedures. Here, based on the recent literature and our own experience, we provide an overview of methods commonly employed for the phenotypic and functional characterization of human \u03b3\u03b4 T cells including advanced polychromatic flow cytometry, mass cytometry, immunohistochemistry, and magnetic cell isolation. We highlight potential pitfalls and discuss how to circumvent these obstacles.<\/jats:p>","DOI":"10.1002\/jlb.5ma1219-296r","type":"journal-article","created":{"date-parts":[[2020,1,22]],"date-time":"2020-01-22T03:38:36Z","timestamp":1579664316000},"page":"1097-1105","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Pitfalls in the characterization of circulating and tissue-resident human \u03b3\u03b4 T cells"],"prefix":"10.1093","volume":"107","author":[{"given":"Nicola","family":"Beucke","sequence":"first","affiliation":[{"name":"Department of Dermatology, University Medical Center , T\u00fcbingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6509-208X","authenticated-orcid":false,"given":"Daniela","family":"Wesch","sequence":"additional","affiliation":[{"name":"Institute of Immunology, Christian-Albrechts University of Kiel , Kiel, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans-Heinrich","family":"Oberg","sequence":"additional","affiliation":[{"name":"Institute of Immunology, Christian-Albrechts University of Kiel , Kiel, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Peters","sequence":"additional","affiliation":[{"name":"Institute of Immunology, Christian-Albrechts University of Kiel , Kiel, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonas","family":"Bochem","sequence":"additional","affiliation":[{"name":"Department of Dermatology, University Medical Center , T\u00fcbingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Weide","sequence":"additional","affiliation":[{"name":"Department of Dermatology, University Medical Center , T\u00fcbingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claus","family":"Garbe","sequence":"additional","affiliation":[{"name":"Department of Dermatology, University Medical Center , T\u00fcbingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3600-0163","authenticated-orcid":false,"given":"Graham","family":"Pawelec","sequence":"additional","affiliation":[{"name":"Interfaculty Institute for Cell Biology, Department of Immunology, University of T\u00fcbingen , T\u00fcbingen, Germany"},{"name":"Health Sciences North Research Institute , Sudbury, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanne","family":"Sebens","sequence":"additional","affiliation":[{"name":"Institute for Experimental Cancer Research, Christian-Albrechts-University of Kiel , Kiel, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christoph","family":"R\u00f6cken","sequence":"additional","affiliation":[{"name":"Institute of Pathology, University Hospital Schleswig-Holstein , Kiel, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hisayoshi","family":"Hashimoto","sequence":"additional","affiliation":[{"name":"Department of Pediatric Hematology and Oncology, University Children's Hospital T\u00fcbingen , T\u00fcbingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Markus W.","family":"L\u00f6ffler","sequence":"additional","affiliation":[{"name":"Interfaculty Institute for Cell Biology, Department of Immunology, University of T\u00fcbingen , T\u00fcbingen, Germany"},{"name":"Department of General, Visceral and Transplant Surgery, University Hospital T\u00fcbingen , T\u00fcbingen, Germany"},{"name":"Department of Clinical Pharmacology, University Hospital T\u00fcbingen , T\u00fcbingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Nocerino","sequence":"additional","affiliation":[{"name":"Systems Cancer Immunology, Comprehensive Cancer Centre, King's College London , London, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shahram","family":"Kordasti","sequence":"additional","affiliation":[{"name":"Systems Cancer Immunology, Comprehensive Cancer Centre, King's College London , London, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dieter","family":"Kabelitz","sequence":"additional","affiliation":[{"name":"Institute of Immunology, Christian-Albrechts University of Kiel , Kiel, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karin","family":"Schilbach","sequence":"additional","affiliation":[{"name":"Department of Pediatric Hematology and Oncology, University Children's Hospital T\u00fcbingen , T\u00fcbingen, 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