{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T09:16:08Z","timestamp":1791191768416,"version":"4.1.0"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[1996,12,1]],"date-time":"1996-12-01T00:00:00Z","timestamp":849398400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1996,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The aim of this work was to study in vivo and in vitro the involvement of macrophages and interleukin-1\u03b2 (IL-l\u03b2) in the necrosis of encapsulated islets during xenograft and to evaluate the immuno-protective efficiency of the AN69 membrane. In vivo, 6 days after implantation, 65% of the membrane surface of the devices containing the islets was colonized with macrophages compared with only 5% of the surface of the empty control devices. The morphological aspect of implanted islets was altered and their insulin release decreased significantly compared with freshly isolated ones (265 \u00b1 50 vs. 507 \u00b1 81 \u03bcU\/mL). In vitro, the insulin release of encapsulated islets cultured for 2 days decreased to 32 and 28%, respectively, in the presence of IL-1\u03b2 and macrophages. The addition of anti-IL-1\u03b2 antibody to the co-culture of macrophages and islets did not modify this loss of functional activity. Furthermore, IL-1\u03b2 passed through the AN69 membrane. In conclusion, macrophages are involved in damaging encapsulated pancreatic islets and are probably partly responsible for islet transplantation failure.<\/jats:p>","DOI":"10.1002\/jlb.60.6.729","type":"journal-article","created":{"date-parts":[[2018,1,12]],"date-time":"2018-01-12T19:01:13Z","timestamp":1515783673000},"page":"729-736","source":"Crossref","is-referenced-by-count":36,"title":["Cytotoxicity of peritoneal murine macrophages against encapsulated pancreatic rat islets: in vivo and in vitro studies"],"prefix":"10.1093","volume":"60","author":[{"given":"Laurence","family":"Kessler","sequence":"first","affiliation":[{"name":"Jeune Equipe M\u00e9dicale Universitaire , and Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cathy","family":"Jesser","sequence":"additional","affiliation":[{"name":"Jeune Equipe M\u00e9dicale Universitaire , and Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yves","family":"Lombard","sequence":"additional","affiliation":[{"name":"Laboratoire d'mmunopharmacologie, FacuLt\u00e9 de Pharmacie , Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V\u00e9ronique","family":"Karsten","sequence":"additional","affiliation":[{"name":"Jeune Equipe M\u00e9dicale Universitaire , and Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alain","family":"Belcourt","sequence":"additional","affiliation":[{"name":"Jeune Equipe M\u00e9dicale Universitaire , and Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel","family":"Pinget","sequence":"additional","affiliation":[{"name":"Jeune Equipe M\u00e9dicale Universitaire , and Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Poindron","sequence":"additional","affiliation":[{"name":"Laboratoire d'mmunopharmacologie, FacuLt\u00e9 de Pharmacie , Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[1996,12,1]]},"container-title":["Journal of Leukocyte Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjlb.60.6.729","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/jleukbio\/article-pdf\/60\/6\/729\/49750315\/jlb0729.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jleukbio\/article-pdf\/60\/6\/729\/49750315\/jlb0729.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,5]],"date-time":"2023-04-05T06:31:15Z","timestamp":1680676275000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jleukbio\/article\/60\/6\/729\/6977145"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,12,1]]},"references-count":0,"journal-issue":{"issue":"6","published-online":{"date-parts":[[1996,12,1]]},"published-print":{"date-parts":[[1996,12,1]]}},"URL":"https:\/\/doi.org\/10.1002\/jlb.60.6.729","relation":{},"ISSN":["0741-5400","1938-3673"],"issn-type":[{"value":"0741-5400","type":"print"},{"value":"1938-3673","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[1996,12]]},"published":{"date-parts":[[1996,12,1]]}}}