{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,5,5]],"date-time":"2023-05-05T11:40:47Z","timestamp":1683286847257},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[1997,2,1]],"date-time":"1997-02-01T00:00:00Z","timestamp":854755200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1997,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Intracellular growth patterns of Legionella pneumophila were examined In monocytes obtained from carriers of human T-lymphotropic virus type I (HTLV-1) and controls who were HTLV-1 seronegative. All subjects were seronegative for antibodies against L. pneumophila. Bacterial growth was determined 0, 1, 2, and\/or 3 days after infecting peripheral blood mononuclear cells (PBMCs) with the bacteria. The intracellular growth of L. pneumophila was markedly inhibited in HTLV-1 carriers compared with normal controls. When the lymphocytes were depleted from the HTLV-1 carrier PBMC cultures before infection, this inhibition was abolished. Inhibition reappeared, however, when the 72-h culture supernatants of PBMCs from HTLV-1 carriers were added to the lymphocyte-depleted cultures. Culture supernatants of infected and uninfected PBMCs from HTLV-1 carriers exhibited markedly increased levels of interferon-\u03b3 (IFN-\u03b3) and tumor necrosis factor \u03b1 (TNF-\u03b1) compared with the HTLV-1 seronegative controls. In the HTLV-1 carriers, IFN-\u03b3 was produced by the CD4+ lymphocytes, whereas TNF-\u03b1 was secreted by the monocytes. Addition of anti-IFN-\u03b3 or anti-TNF-\u03b1 antibodies to the HTLV-1 carrier PBMC cultures diminished the inhibition of intracellular growth of L. pneumophila. Results suggest that the monocytes are activated in HTLV-1 carriers. These findings may explain why an opportunistic infection by certain intracellular pathogens is rarely seen among HTLV-1 carriers.<\/jats:p>","DOI":"10.1002\/jlb.61.2.133","type":"journal-article","created":{"date-parts":[[2018,1,12]],"date-time":"2018-01-12T19:04:39Z","timestamp":1515783879000},"page":"133-140","source":"Crossref","is-referenced-by-count":0,"title":["Marked inhibition of the intracellular multiplication of <i>Legionella pneumophila<\/i> in monocytes isolated from carriers of human T lymphotropic virus type I"],"prefix":"10.1093","volume":"61","author":[{"given":"Noriko","family":"Funai","sequence":"first","affiliation":[{"name":"Division of Hematology, Department of Internal Medicine, Saga Medical School , Saga, Japan"},{"name":"Department of Microbiology, Saga Medical School , Saga, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshinori","family":"Shimamoto","sequence":"additional","affiliation":[{"name":"Division of Hematology, Department of Internal Medicine, Saga Medical School , Saga, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masayuki","family":"Watanabe","sequence":"additional","affiliation":[{"name":"Division of Hematology, Department of Internal Medicine, Saga Medical School , Saga, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shin-lchi","family":"Yoshida","sequence":"additional","affiliation":[{"name":"Division of Hematology, Department of Internal Medicine, Saga Medical School , Saga, Japan"},{"name":"Department of Microbiology, School of Medicine, University of Occupational and Environmental Health , Kitakyushu, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Osamu","family":"Kohashi","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Saga Medical School , Saga, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[1997,2,1]]},"container-title":["Journal of Leukocyte Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjlb.61.2.133","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/jleukbio\/article-pdf\/61\/2\/133\/50203164\/jlb0133.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jleukbio\/article-pdf\/61\/2\/133\/50203164\/jlb0133.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,5]],"date-time":"2023-05-05T11:11:33Z","timestamp":1683285093000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jleukbio\/article\/61\/2\/133\/6976867"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,2,1]]},"references-count":0,"journal-issue":{"issue":"2","published-online":{"date-parts":[[1997,2,1]]},"published-print":{"date-parts":[[1997,2,1]]}},"URL":"https:\/\/doi.org\/10.1002\/jlb.61.2.133","relation":{},"ISSN":["0741-5400","1938-3673"],"issn-type":[{"value":"0741-5400","type":"print"},{"value":"1938-3673","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[1997,2]]},"published":{"date-parts":[[1997,2,1]]}}}