{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T18:40:28Z","timestamp":1760985628535},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[1997,4,1]],"date-time":"1997-04-01T00:00:00Z","timestamp":859852800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1997,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>First-generation replication-defective adenoviruses have been reported to lead to transient reporter gene expression due to a specific immune reaction involving T and B lymphocytes. Some recent reports have also demonstrated the presence of a nonspecific inflammatory reaction involving macrophages and neutrophils after both intramuscular injections and viral vectors transduction. To further investigate this nonspecific inflammatory reaction, \u0394E1\/E3a adenoviruses were injected intramuscularly in immunocompetent mice. Some of these mice were treated with anti-LFA-1. The adenovirus-injected muscles showed abundant CD4+, CD8+, LFA-1+, and Mac-1+ cell infiltration 3 days after the \u0394E1\/E3a injection. The anti-LFA-1 monoclonal antibody was able to block the nonspecific inflammatory damage due mostly to neutrophils and macrophages. The anti-LFA-1 did not produce this effect by reducing the muscle infiltration by LFA-1+ cells. It may instead have blocked the direct interaction between LFA-1 and ICAM-1 thus preventing the damage produced by the respiratory burst of neutrophils. Blocking the resulting damage of this inflammatory reaction with anti-LFA-1 in animals also treated with FK506, a powerful immunosuppressant for gene therapy, largely increased the long-term transgene expression compared with mice only treated with FK506.<\/jats:p>","DOI":"10.1002\/jlb.61.4.533","type":"journal-article","created":{"date-parts":[[2018,1,12]],"date-time":"2018-01-12T19:05:14Z","timestamp":1515783914000},"page":"533-538","source":"Crossref","is-referenced-by-count":10,"title":["Inflammatory damage following first-generation replication-defective adenovirus controlled by anti-LFA-1"],"prefix":"10.1093","volume":"61","author":[{"given":"B","family":"Gu\u00e9rette","sequence":"first","affiliation":[{"name":"Centre de Recherche en Neurobiologie, Universit\u00e9 Laval and H\u00f4pital de l'Enfant-J\u00e9sus , Qu\u00e9bec"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P-A","family":"Moisset","sequence":"additional","affiliation":[{"name":"Centre de Recherche en Neurobiologie, Universit\u00e9 Laval and H\u00f4pital de l'Enfant-J\u00e9sus , Qu\u00e9bec"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"Huard","sequence":"additional","affiliation":[{"name":"Centre de Recherche en Neurobiologie, Universit\u00e9 Laval and H\u00f4pital de l'Enfant-J\u00e9sus , Qu\u00e9bec"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F","family":"Tardif","sequence":"additional","affiliation":[{"name":"Centre de Recherche en Neurobiologie, Universit\u00e9 Laval and H\u00f4pital de l'Enfant-J\u00e9sus , Qu\u00e9bec"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"Gravel","sequence":"additional","affiliation":[{"name":"Laboratoire de Transfert de G\u00e8nes, Centre de Recherche Universit\u00e9 Laval Robert-Giffard , Beauport, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J P","family":"Tremblay","sequence":"additional","affiliation":[{"name":"Centre de Recherche en Neurobiologie, Universit\u00e9 Laval and H\u00f4pital de l'Enfant-J\u00e9sus , Qu\u00e9bec"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[1997,4,1]]},"container-title":["Journal of Leukocyte Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjlb.61.4.533","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/jleukbio\/article-pdf\/61\/4\/533\/49749990\/jlb0533.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jleukbio\/article-pdf\/61\/4\/533\/49749990\/jlb0533.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,5]],"date-time":"2023-04-05T06:20:04Z","timestamp":1680675604000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jleukbio\/article\/61\/4\/533\/6976910"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,4,1]]},"references-count":0,"journal-issue":{"issue":"4","published-online":{"date-parts":[[1997,4,1]]},"published-print":{"date-parts":[[1997,4,1]]}},"URL":"https:\/\/doi.org\/10.1002\/jlb.61.4.533","relation":{},"ISSN":["0741-5400","1938-3673"],"issn-type":[{"value":"0741-5400","type":"print"},{"value":"1938-3673","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[1997,4]]},"published":{"date-parts":[[1997,4,1]]}}}