{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T12:12:56Z","timestamp":1770725576028,"version":"3.49.0"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[1997,6,1]],"date-time":"1997-06-01T00:00:00Z","timestamp":865123200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1997,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Tumor necrosis factor-\u03b1 (TNF-\u03b1) is a potent proinflammatory cytokine and mediator of the inflammatory response. It has been implicated in the pathogenesis of many inflammatory disorders, including rheumatoid arthritis (RA), septic shock, and Crohn's disease. Using a specific anti-human TNF-\u03b1 antibody we detected immunoreactivity for this cytokine in the cytoplasm of inflammatory cells in several chronic inflammatory disorders, including RA, scleritis, and polyarteritis nodosa. These cells were identified predominantly as IgG-expressing plasma cells. Lymph nodes from patients with Hodgkin's lymphoma and breast cancer, but not from control subjects, were also found to contain TNF-\u03b1-positive plasma cells. Cultured EBV-B lymphocytes and a human plasma cell line (ARH-77) when stimulated with phorbol myristate acetate demonstrated cytoplasmic TNF-\u03b1 immunoreactivity. Western blot analysis of cell membranes and conditioned media from both cell types revealed the presence of the 26-kDa membrane-bound form and the 17-kDa soluble form of TNF-\u03b1, respectively. TNF-\u03b1 was quantitated by enzymelinked immunosorbent assay and found to be biologically active as determined by the L929 cytotoxicity assay. This is the first demonstration that plasma cells may be capable of modulating immune and inflammatory responses, not only by antibody production, but also by their secretion of a key inflammatory mediator, TNF-\u03b1. J.<\/jats:p>","DOI":"10.1002\/jlb.61.6.667","type":"journal-article","created":{"date-parts":[[2018,1,12]],"date-time":"2018-01-12T19:04:08Z","timestamp":1515783848000},"page":"667-678","source":"Crossref","is-referenced-by-count":51,"title":["Expression of TNF-\u03b1 by human plasma cells in chronic inflammation"],"prefix":"10.1093","volume":"61","author":[{"given":"Nick","family":"Di Girolamo","sequence":"first","affiliation":[{"name":"Inflammation Research Unit, School of Pathology, The University of New South Wales , Sydney, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kumar","family":"Visvanathan","sequence":"additional","affiliation":[{"name":"Inflammation Research Unit, School of Pathology, The University of New South Wales , Sydney, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew","family":"Lloyd","sequence":"additional","affiliation":[{"name":"Inflammation Research Unit, School of Pathology, The University of New South Wales , Sydney, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Denis","family":"Wakefield","sequence":"additional","affiliation":[{"name":"Inflammation Research Unit, School of Pathology, The University of New South Wales , Sydney, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[1997,6,1]]},"container-title":["Journal of Leukocyte Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjlb.61.6.667","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/jleukbio\/article-pdf\/61\/6\/667\/49749771\/jlb0667.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jleukbio\/article-pdf\/61\/6\/667\/49749771\/jlb0667.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,5]],"date-time":"2023-04-05T05:53:58Z","timestamp":1680674038000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jleukbio\/article\/61\/6\/667\/6977081"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,6,1]]},"references-count":0,"journal-issue":{"issue":"6","published-online":{"date-parts":[[1997,6,1]]},"published-print":{"date-parts":[[1997,6,1]]}},"URL":"https:\/\/doi.org\/10.1002\/jlb.61.6.667","relation":{},"ISSN":["0741-5400","1938-3673"],"issn-type":[{"value":"0741-5400","type":"print"},{"value":"1938-3673","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[1997,6]]},"published":{"date-parts":[[1997,6,1]]}}}