{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T07:25:58Z","timestamp":1770708358382,"version":"3.49.0"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[1999,2,1]],"date-time":"1999-02-01T00:00:00Z","timestamp":917827200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1999,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Among the phagocytic leukocytes, monocytes have the important role of clearing out parasitic microorganisms. They accomplish this through production of toxic metabolites of oxygen. Trp-Lys-Tyr-Met-Val-D-Met (WKYMVm), a peptide that stimulates phosphoinositide (PI) hydrolysis in human leukocytes, including monocytes, binds to a unique cell surface receptor and stimulates superoxide generation, killing of Staphylococcus aureus, and activation of phospholipase D (PLD) in human monocytes. Preincubation of the cells with a PI-specific phospholipase C (PLC) inhibitor (U-73122), protein kinase C inhibitor (GF109203X), or intracellular Ca2+ chelator (BAPTA\/AM) before the peptide stimulus totally inhibits the peptide-induced PLD activation and superoxide generation. On the other hand, tyrosine kinase inhibitor genistein only partially inhibits the peptide-induced processes. The peptide-induced bacteria killing activity shares regulatory mechanisms for PLD activation with the superoxide generation, which is inhibited in the presence of 1-butanol. We suggest that the peptide stimulates PLD downstream of PLC activation and PLD activation in turn is essential for the peptide-induced immunological functions such as the superoxide generation and killing of bacteria by human monocytes. J. Leukoc. Biol. 65:241\u2013248; 1999.<\/jats:p>","DOI":"10.1002\/jlb.65.2.241","type":"journal-article","created":{"date-parts":[[2018,1,12]],"date-time":"2018-01-12T18:51:19Z","timestamp":1515783079000},"page":"241-248","source":"Crossref","is-referenced-by-count":44,"title":["Trp-Lys-Tyr-Met-Val-D-Met stimulates superoxide generation and killing of <i>Staphylococcus aureus<\/i> via phospholipase D activation in human monocytes"],"prefix":"10.1093","volume":"65","author":[{"given":"Yoe-Sik","family":"Bae","sequence":"first","affiliation":[{"name":"Department of Life Science, Pohang University of Science and Technology , Pohang, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seong-A","family":"Ju","sequence":"additional","affiliation":[{"name":"Department of Biology, Ulsan University , Ulsan, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ji Yung","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Biology, Ulsan University , Ulsan, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeong Kon","family":"Seo","sequence":"additional","affiliation":[{"name":"Department of Life Science, Pohang University of Science and Technology , Pohang, Korea"},{"name":"Ludwig Institute for Cancer Research Present address of Jeong Kon Seo: , Box 595, S-75124 Uppsala, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suk Hwan","family":"Baek","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, College of Medicine, Yeung Nam University , Taegu, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jong-Young","family":"Kwak","sequence":"additional","affiliation":[{"name":"College of Medicine, Dong A University , Pusan, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Byung Sam","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Biology, Ulsan University , Ulsan, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pann-Ghill","family":"Suh","sequence":"additional","affiliation":[{"name":"Department of Life Science, Pohang University of Science and Technology , Pohang, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sung Ho","family":"Ryu","sequence":"additional","affiliation":[{"name":"Department of Life Science, Pohang University of Science and Technology , Pohang, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[1999,2,1]]},"container-title":["Journal of Leukocyte Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjlb.65.2.241","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/jleukbio\/article-pdf\/65\/2\/241\/49732169\/jlb0241.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jleukbio\/article-pdf\/65\/2\/241\/49732169\/jlb0241.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,5]],"date-time":"2023-04-05T04:30:50Z","timestamp":1680669050000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jleukbio\/article\/65\/2\/241\/6976732"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,2,1]]},"references-count":0,"journal-issue":{"issue":"2","published-online":{"date-parts":[[1999,2,1]]},"published-print":{"date-parts":[[1999,2,1]]}},"URL":"https:\/\/doi.org\/10.1002\/jlb.65.2.241","relation":{},"ISSN":["0741-5400","1938-3673"],"issn-type":[{"value":"0741-5400","type":"print"},{"value":"1938-3673","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[1999,2]]},"published":{"date-parts":[[1999,2,1]]}}}