{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T08:37:47Z","timestamp":1790411867104,"version":"4.1.0"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2000,3,1]],"date-time":"2000-03-01T00:00:00Z","timestamp":951868800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2000,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The psychoactive component of marijuana, \u03b4-tetrahydrocannabinol (THC) suppresses different functions of immunocytes, including the antimicrobicidal activity of macrophages. The triggering of cannabinoid receptors of CB1 and CB2 subtypes present on leukocytes may account for these effects. We investigated the influence of specific CB1 or CB2 receptor antagonists (SR141716A and SR144528, respectively) and nonselective CB1\/CB2 cannabinoid receptor agonists (CP55,940 or WIN 55212-2) on macrophage infection by Brucella suis, an intracellular gram-negative bacteria. None of the compounds tested affected bacterial phagocytosis. By contrast, the intracellular multiplication of Brucella was dosedependently inhibited in cells treated with 10\u2013500 nM SR141716A and 1 \u03bcM SR141716A-induced cells exerted a potent microbicidal effect against the bacteria. SR144528, CP55,940, or WIN 55212-2 did not affect (or slightly potentiated) the growth of phagocytized bacteria. However, CP55,940 or WIN 55212-2 reversed the SR141716A-mediated effect, which strongly suggested an involvement of macrophage CB1 receptors in the phenomenon. SR141716A was able to pre-activate macrophages and to trigger an activation signal that inhibited Brucella development. The participation of endogenous cannabinoid ligand(s) in Brucella infection was discussed. Finally, our data show that SR141716A up-regulates the antimicrobial properties of macrophages in vitro and might be a pharmaceutical compound useful for counteracting the development of intramacrophagic gram-negative bacteria. J. Leukoc. Biol. 67: 335\u2013344; 2000.<\/jats:p>","DOI":"10.1002\/jlb.67.3.335","type":"journal-article","created":{"date-parts":[[2018,1,12]],"date-time":"2018-01-12T18:52:43Z","timestamp":1515783163000},"page":"335-344","source":"Crossref","is-referenced-by-count":23,"title":["A beneficial aspect of a CB1 cannabinoid receptor antagonist: SR141716A is a potent inhibitor of macrophage infection by the intracellular pathogen Brucella suis"],"prefix":"10.1093","volume":"67","author":[{"given":"Antoine","family":"Gross","sequence":"first","affiliation":[{"name":"INSERM U431, IFR Eug\u00e8ne Bataillon, Universit\u00e9 de Montpellier-II , Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annie","family":"Terraza","sequence":"additional","affiliation":[{"name":"INSERM U431, IFR Eug\u00e8ne Bataillon, Universit\u00e9 de Montpellier-II , Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean","family":"Marchant","sequence":"additional","affiliation":[{"name":"Sanofi Recherche , Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monsif","family":"Bouaboula","sequence":"additional","affiliation":[{"name":"Sanofi Recherche , Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Safia","family":"Ouahrani-Bettache","sequence":"additional","affiliation":[{"name":"INSERM U431, IFR Eug\u00e8ne Bataillon, Universit\u00e9 de Montpellier-II , Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Pierre","family":"Liautard","sequence":"additional","affiliation":[{"name":"INSERM U431, IFR Eug\u00e8ne Bataillon, Universit\u00e9 de Montpellier-II , Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Casellas","sequence":"additional","affiliation":[{"name":"Sanofi Recherche , Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacques","family":"Dornand","sequence":"additional","affiliation":[{"name":"INSERM U431, IFR Eug\u00e8ne Bataillon, Universit\u00e9 de Montpellier-II , Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2000,3,1]]},"container-title":["Journal of Leukocyte Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjlb.67.3.335","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/jleukbio\/article-pdf\/67\/3\/335\/49700630\/jlb0335.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jleukbio\/article-pdf\/67\/3\/335\/49700630\/jlb0335.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,3,30]],"date-time":"2023-03-30T23:50:00Z","timestamp":1680220200000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jleukbio\/article\/67\/3\/335\/6926761"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,3,1]]},"references-count":0,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2000,3,1]]},"published-print":{"date-parts":[[2000,3,1]]}},"URL":"https:\/\/doi.org\/10.1002\/jlb.67.3.335","relation":{},"ISSN":["0741-5400","1938-3673"],"issn-type":[{"value":"0741-5400","type":"print"},{"value":"1938-3673","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2000,3]]},"published":{"date-parts":[[2000,3,1]]}}}