{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T06:03:03Z","timestamp":1760853783252,"version":"build-2065373602"},"reference-count":45,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2014,7,28]],"date-time":"2014-07-28T00:00:00Z","timestamp":1406505600000},"content-version":"vor","delay-in-days":27,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000070","name":"the National Institute of Biomedical Imaging and Bioengineering","doi-asserted-by":"crossref","award":["R21 EB017237-01"],"award-info":[{"award-number":["R21 EB017237-01"]}],"id":[{"id":"10.13039\/100000070","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Labelled Comp Radiopharmac"],"published-print":{"date-parts":[[2014,7]]},"abstract":"<jats:p>The objective of this study was to develop a kit formulation for [<jats:sup>99m<\/jats:sup>TcN(mpo)(PNP5)]<jats:sup>+<\/jats:sup> (MPO\u2009=\u20092\u2010mercaptopyridine oxide), (<jats:sup>99m<\/jats:sup>TcN\u2010MPO) to support its clinical evaluations as a SPECT radiotracer. Radiolabeling studies were performed using three different formulations (two\u2010vial formulation and single\u2010vial formulations with\/without SnCl<jats:sub>2<\/jats:sub>) to explore the factors influencing radiochemical purity (RCP) of <jats:sup>99m<\/jats:sup>TcN\u2010MPO. We found that the most important factor affecting the RCP of <jats:sup>99m<\/jats:sup>TcN\u2010MPO was the purity of PNP5. <jats:sup>99m<\/jats:sup>TcN\u2010MPO was prepared &gt;98% RCP (<jats:italic>n<\/jats:italic>\u2009=\u200920) using the two\u2010vial formulation. For single\u2010vial formulations with\/without SnCl<jats:sub>2<\/jats:sub>, <jats:italic>\u03b2<\/jats:italic>\u2010cyclodextrin (<jats:italic>\u03b2<\/jats:italic>\u2010CD) is particularly useful as a stabilizer for PNP5. The RCP of <jats:sup>99m<\/jats:sup>TcN\u2010MPO was 95\u201398% using <jats:italic>\u03b2<\/jats:italic>\u2010CD, but its RCP was only 90\u201393% with <jats:italic>\u03b3<\/jats:italic>\u2010cyclodextrin (<jats:italic>\u03b3<\/jats:italic>\u2010CD). It seems that PNP5 fits better into the inner cavity of <jats:italic>\u03b2<\/jats:italic>\u2010CD, which forms more stable inclusion complex than <jats:italic>\u03b3<\/jats:italic>\u2010CD in the single\u2010vial formulations. The results from biodistribution and imaging studies in Sprague\u2013Dawley rats clearly demonstrated biological equivalence of three different formulations. Single photon\u2010emission computed tomography data suggested that high quality images could be obtained at 0\u201330\u2010min post\u2010injection without significant interference from the liver radioactivity. Considering the ease for <jats:sup>99m<\/jats:sup>Tc\u2010labeling and high RCP of <jats:sup>99m<\/jats:sup>TcN\u2010MPO, the non\u2010SnCl<jats:sub>2<\/jats:sub> single\u2010vial formulation is an attractive choice for future clinical studies.<\/jats:p>","DOI":"10.1002\/jlcr.3221","type":"journal-article","created":{"date-parts":[[2014,7,28]],"date-time":"2014-07-28T12:16:39Z","timestamp":1406549799000},"page":"584-592","source":"Crossref","is-referenced-by-count":7,"title":["Development of kit formulations for <sup>99m<\/sup>TcN\u2010MPO: a cationic radiotracer for myocardial perfusion imaging"],"prefix":"10.1002","volume":"57","author":[{"given":"Yumin","family":"Zheng","sequence":"first","affiliation":[{"name":"School of Health Sciences Purdue University  West Lafayette IN 47907 USA"},{"name":"Department of Nuclear Medicine China\u2010Japan Friendship Hospital  Beijing 100029 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shundong","family":"Ji","sequence":"additional","affiliation":[{"name":"School of Health Sciences Purdue University  West Lafayette IN 47907 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"Tomaselli","sequence":"additional","affiliation":[{"name":"School of Health Sciences Purdue University  West Lafayette IN 47907 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuang","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Health Sciences Purdue University  West Lafayette IN 47907 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,7,28]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1067\/mnc.2000.109969"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1071-3581(00)90042-4"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.101.12.1465"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1053\/snuc.2001.23529"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00259-002-0998-8"},{"key":"e_1_2_9_7_1","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1093\/bmb\/ldl014","volume":"79","author":"Henneman M. 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