{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T16:14:06Z","timestamp":1790266446818,"version":"4.1.0"},"reference-count":28,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2016,12,7]],"date-time":"2016-12-07T00:00:00Z","timestamp":1481068800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"publisher","award":["BK20151118"],"award-info":[{"award-number":["BK20151118"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"publisher","award":["BK20141102"],"award-info":[{"award-number":["BK20141102"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["21001055"],"award-info":[{"award-number":["21001055"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["21371082"],"award-info":[{"award-number":["21371082"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Labelled Comp Radiopharmac"],"published-print":{"date-parts":[[2017,2]]},"abstract":"<jats:p>Technetium\u201099m\u2010based radiopharmaceuticals have been used widely as diagnostic agents in the nuclear medicine. Chlorambucil (CLB) as one typical alkylating drug exhibits excellent inhibition effects against many human malignancies. To develop and explore a novel potential imaging agent for early diagnosis of tumors, tricarbonyl technetium\u201099m and rhenium complexes on the basis of the tridentate ligand dipicolylamine (DPA) bound to the chlorambucil pharmacophore were designed and synthesized: <jats:sup>99m<\/jats:sup>Tc\u2010DPA\u2010CLB (<jats:bold>3<\/jats:bold>) and Re\u2010DPA\u2010CLB (<jats:bold>4<\/jats:bold>). The high performance liquid chromatography analyses showed that the retention time of <jats:bold>3<\/jats:bold> and <jats:bold>4<\/jats:bold> was 13.5 and 13.6\u00a0minutes, respectively. Radiolabeling efficiency of the <jats:sup>99m<\/jats:sup>Tc\u2010DPA\u2010CLB tracer was 97%, and the radiochemical purity was larger than 95% after 6\u00a0hours stored in phosphate buffered saline or human serum as observed by thin layer chromatography and high performance liquid chromatography. Biodistribution studies in a mouse model of breast cancer showed <jats:sup>99m<\/jats:sup>Tc\u2010DPA\u2010CLB exhibited a favorable tumor affinity. The radiotracer cleared quickly in the first hour via hepatobiliary and renal routes of excretion, resulted in a very low background at 4\u00a0hours post injection (p.i.). It had moderate uptake ratios of tumor to blood and tumor to muscle. These results suggested <jats:sup>99m<\/jats:sup>Tc\u2010DPA\u2010CLB might be a promising SPECT imaging agent for tumor diagnosis.<\/jats:p>","DOI":"10.1002\/jlcr.3481","type":"journal-article","created":{"date-parts":[[2016,11,16]],"date-time":"2016-11-16T14:42:48Z","timestamp":1479307368000},"page":"116-123","source":"Crossref","is-referenced-by-count":14,"title":["Synthesis and preliminary biological evaluation of a <scp><sup>99m<\/sup>Tc\u2010chlorambucil<\/scp> derivative as a potential tumor imaging agent"],"prefix":"10.1002","volume":"60","author":[{"given":"Jianguo","family":"Lin","sequence":"first","affiliation":[{"name":"Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Key Laboratory of Molecular Nuclear Medicine Jiangsu Institute of Nuclear Medicine  Wuxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Qiu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Key Laboratory of Molecular Nuclear Medicine Jiangsu Institute of Nuclear Medicine  Wuxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaochao","family":"Lv","sequence":"additional","affiliation":[{"name":"Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Key Laboratory of Molecular Nuclear Medicine Jiangsu Institute of Nuclear Medicine  Wuxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ke","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Key Laboratory of Molecular Nuclear Medicine Jiangsu Institute of Nuclear Medicine  Wuxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Key Laboratory of Molecular Nuclear Medicine Jiangsu Institute of Nuclear Medicine  Wuxi 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