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This requires the liquid chromatography tandem mass spectrometry (LCMS\/MS) measurement of sulfated <jats:italic>N<\/jats:italic>\u2010acetyl\u2010<jats:sc>d<\/jats:sc>\u2010lactosamines in the urine after cleavage of the keratan sulfate with keratanase II. Quantification requires isotopically\u2010labelled internal standards. The synthesis of these <jats:sup>13<\/jats:sup>C<jats:sub>6<\/jats:sub>\u2010labelled standards from <jats:sup>13<\/jats:sup>C<jats:sub>6<\/jats:sub>\u2010galactose and <jats:italic>N<\/jats:italic>\u2010acetylglucosamine is described. The required protected disaccharide is prepared utilising a regioselective, high yielding \u03b2\u2010galactosylation of a partially protected glucosamine acceptor and an inverse addition protocol. 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