{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T00:37:52Z","timestamp":1760661472219,"version":"build-2065373602"},"reference-count":15,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,2,27]],"date-time":"2019-02-27T00:00:00Z","timestamp":1551225600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Wolfson Brain Imaging Centre, Department of Clinical Neurosciences, University of Cambridge, Addenbrooke's Hospital, Cambridge CB2 0QQ, United Kingdom"},{"name":"Medical Research Council","award":["MR\/K02308X\/1","NIHR EME 14\/145\/09"],"award-info":[{"award-number":["MR\/K02308X\/1","NIHR EME 14\/145\/09"]}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Labelled Comp Radiopharmac"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:p>[<jats:sup>11<\/jats:sup>C]metomidate ([<jats:sup>11<\/jats:sup>C]MTO) is a radiotracer widely used to detect disorders of adrenocortical origin by positron emission tomography (PET) imaging. [<jats:sup>11<\/jats:sup>C]MTO PET\/computed tomography (PET\/CT) is considered a sensitive and specific noninvasive alternative to adrenal vein sampling (AVS) in the management of primary hyperaldosteronism (PHA). Herein, we report a reliable automated procedure for the routine manufacturing of [<jats:sup>11<\/jats:sup>C]MTO in current good manufacturing practice (cGMP) conditions on the commercial Synthra MeI<jats:sub>Plus<\/jats:sub> Loop Vessel synthesizer. The method is based on a combination of the captive\u2010solvent <jats:sup>11<\/jats:sup>C\u2010methylation of the carboxylate salt <jats:bold>1b<\/jats:bold> of the MTO precursor <jats:bold>1a<\/jats:bold> followed by solid phase extraction (SPE) cartridge purification methodology, which substitutes HPLC purification of the crude reaction mixture. Starting from 45\u00a0GBq [<jats:sup>11<\/jats:sup>C]CO<jats:sub>2<\/jats:sub> at the end of bombardment (EOB), 3\u00a0GBq of pure [<jats:sup>11<\/jats:sup>C]MTO was produced in 18\u00a0minutes with 12% decay corrected radiochemical yield (RCY) at the end of synthesis (EOS) and with the modest molar activity of 13\u00a0GBq\/\u03bcmol at the time of application. Each dose produced met all established quality control (QC) criteria. The method can easily be implemented into other commercial automated radiosynthesizers for manufacturing carbon\u201011 labeled radiotracers.<\/jats:p>","DOI":"10.1002\/jlcr.3711","type":"journal-article","created":{"date-parts":[[2019,1,28]],"date-time":"2019-01-28T22:34:32Z","timestamp":1548714872000},"page":"190-197","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["A simple and efficient automated cGMP\u2010compliant radiosynthesis of [<sup>11<\/sup>C]metomidate using solid phase extraction cartridge purification"],"prefix":"10.1002","volume":"62","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8631-8308","authenticated-orcid":false,"given":"Mitja","family":"Kovac","sequence":"first","affiliation":[{"name":"Wolfson Brain Imaging Centre, Department of Clinical Neurosciences University of Cambridge, Addenbrooke's Hospital  Cambridge CB2 0QQ UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tunde","family":"Miklovicz","sequence":"additional","affiliation":[{"name":"Wolfson Brain Imaging Centre, Department of Clinical Neurosciences University of Cambridge, Addenbrooke's Hospital  Cambridge CB2 0QQ UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Li","sequence":"additional","affiliation":[{"name":"Wolfson Brain Imaging Centre, Department of Clinical Neurosciences University of Cambridge, Addenbrooke's Hospital  Cambridge CB2 0QQ UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph","family":"Russell","sequence":"additional","affiliation":[{"name":"Wolfson Brain Imaging Centre, Department of Clinical Neurosciences University of Cambridge, Addenbrooke's Hospital  Cambridge CB2 0QQ UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberto","family":"Canales Candela","sequence":"additional","affiliation":[{"name":"Wolfson Brain Imaging Centre, Department of Clinical Neurosciences University of Cambridge, Addenbrooke's Hospital  Cambridge CB2 0QQ UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Franklin","family":"Aigbirhio","sequence":"additional","affiliation":[{"name":"Wolfson Brain Imaging Centre, Department of Clinical Neurosciences University of Cambridge, Addenbrooke's Hospital  Cambridge CB2 0QQ UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Istvan","family":"Boros","sequence":"additional","affiliation":[{"name":"Wolfson Brain Imaging Centre, Department of Clinical Neurosciences University of Cambridge, Addenbrooke's Hospital  Cambridge CB2 0QQ UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,2,27]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2003-031337"},{"issue":"6","key":"e_1_2_7_3_1","first-page":"982","article-title":"In vitro and in vivo primate evaluation of Carbon\u201011\u2010etomidate and Carbon\u201011\u2010Metomidate as potential tracers for PET imaging of the adrenal cortex and its tumors","volume":"39","author":"Bergstr\u00f6m M","year":"1998","journal-title":"J Nucl Med"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2005-2273"},{"issue":"2","key":"e_1_2_7_5_1","first-page":"275","article-title":"PET imaging of adrenal cortical tumors with the 11beta\u2010hydroxylase tracer 11C\u2010metomidate","volume":"41","author":"Bergstr\u00f6m M","year":"2000","journal-title":"J Nucl Med"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00259-002-1025-9"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2011-1537"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/diagnostics6040042"},{"key":"e_1_2_7_9_1","first-page":"245","volume-title":"Radiochemical Syntheses: Radiopharmaceuticals for Positron Emission Tomography, Volume 1, Chapter 24: Synthesis of O\u2010methyl\u2010[11C]Metomidate ([11C]MTO). Hoboken","author":"Boros I","year":"2012"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1258\/002367781780958919"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0969-8043(03)00168-4"},{"issue":"6","key":"e_1_2_7_12_1","first-page":"972","article-title":"Imaging of adrenal Incidentalomas with PET using 11C\u2010Metomidate and 18F\u2010FDG","volume":"45","author":"Minn H","year":"2004","journal-title":"J Nucl Med"},{"key":"e_1_2_7_13_1","first-page":"S313","article-title":"Captive solvent methylations in the injector loop of the Tracerlab Carbon\u201011 module","volume":"52","author":"Kuznetsov M","year":"2009","journal-title":"J Labelled Comp Radiopharm"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apradiso.2008.09.012"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nucmedbio.2018.09.003"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nucmedbio.2017.11.006"}],"container-title":["Journal of Labelled Compounds and Radiopharmaceuticals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjlcr.3711","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jlcr.3711","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T19:23:00Z","timestamp":1760642580000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jlcr.3711"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,2,27]]},"references-count":15,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2019,4]]}},"alternative-id":["10.1002\/jlcr.3711"],"URL":"https:\/\/doi.org\/10.1002\/jlcr.3711","archive":["Portico"],"relation":{},"ISSN":["0362-4803","1099-1344"],"issn-type":[{"type":"print","value":"0362-4803"},{"type":"electronic","value":"1099-1344"}],"subject":[],"published":{"date-parts":[[2019,2,27]]},"assertion":[{"value":"2018-11-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-01-21","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-02-27","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}